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The map label for this gene is pncA [H]

Identifier: 145296489

GI number: 145296489

Start: 2637783

End: 2638343

Strand: Direct

Name: pncA [H]

Synonym: cgR_2400

Alternate gene names: 145296489

Gene position: 2637783-2638343 (Clockwise)

Preceding gene: 145296488

Following gene: 145296491

Centisome position: 79.59

GC content: 54.37

Gene sequence:

>561_bases
ATGGCACGCGCACTCATTCTGGTTGATGTTCAAAAAGACTTCTGCCCCGGTGGCAGCCTAGCCACCGAACGAGGCGATGA
AGTGGCAGGAAAAATCGGTGCCTATCAGCTGTCCCACGGCTCAGAGTACGACGTCGTTGTGGCGACCCAAGATTGGCACA
TCGATCCAGGCGAGCACTTTTCAGAAACCCCAGACTTTAAAAACTCCTGGCCAATCCACTGCGTCGCGGATTCCGATGGT
GCCGCCATGCATGACCGCATCAACACCGATTCAATCGATGAGTTCTTCCGCAAAGGCCATTACACCGCGGCGTATTCCGG
GTTCGAGGGAACTGCAGTCAGTGAAGAACTCCTCATGTCTCCATGGCTGAAGAACAAGGGAGTCACTGATGTAGACATCG
TGGGGATCGCTACGGATCACTGCGTTCGAGCCACAGCACTTGATGCTCTCAAGGAGGGCTTCAACGTTCGCGTTTTGACG
TCGATGTGTTCTGCGGTGGATTTCCATGCGGGAGATCACGCATTGGAGGAACTGCATGAAGCCGGGGCGATCCTGATTTA
A

Upstream 100 bases:

>100_bases
GACCGCGCCCTCAACAAAGAAGACTAGAAGCGCACAGGTTTTTGCATGGCTAGACTTTGAATGAGTAAGCACGAACACAT
TTCAAAGGAAGGGTTTCACC

Downstream 100 bases:

>100_bases
AGAAACCTTAAACCACGAAACGCCCCGTCGAAAAGCAATTTAACGCTCGGAGAGCAGACGCTGGATTTCCTTCAGATCGT
TTGCCTTCTTGCGGCCACGC

Product: hypothetical protein

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MARALILVDVQKDFCPGGSLATERGDEVAGKIGAYQLSHGSEYDVVVATQDWHIDPGEHFSETPDFKNSWPIHCVADSDG
AAMHDRINTDSIDEFFRKGHYTAAYSGFEGTAVSEELLMSPWLKNKGVTDVDIVGIATDHCVRATALDALKEGFNVRVLT
SMCSAVDFHAGDHALEELHEAGAILI

Sequences:

>Translated_186_residues
MARALILVDVQKDFCPGGSLATERGDEVAGKIGAYQLSHGSEYDVVVATQDWHIDPGEHFSETPDFKNSWPIHCVADSDG
AAMHDRINTDSIDEFFRKGHYTAAYSGFEGTAVSEELLMSPWLKNKGVTDVDIVGIATDHCVRATALDALKEGFNVRVLT
SMCSAVDFHAGDHALEELHEAGAILI
>Mature_185_residues
ARALILVDVQKDFCPGGSLATERGDEVAGKIGAYQLSHGSEYDVVVATQDWHIDPGEHFSETPDFKNSWPIHCVADSDGA
AMHDRINTDSIDEFFRKGHYTAAYSGFEGTAVSEELLMSPWLKNKGVTDVDIVGIATDHCVRATALDALKEGFNVRVLTS
MCSAVDFHAGDHALEELHEAGAILI

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=204, Percent_Identity=36.2745098039216, Blast_Score=117, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321401, Length=193, Percent_Identity=27.4611398963731, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648247, Length=221, Percent_Identity=27.1493212669683, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 20178; Mature: 20046

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARALILVDVQKDFCPGGSLATERGDEVAGKIGAYQLSHGSEYDVVVATQDWHIDPGEHF
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCC
SETPDFKNSWPIHCVADSDGAAMHDRINTDSIDEFFRKGHYTAAYSGFEGTAVSEELLMS
CCCCCCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHC
PWLKNKGVTDVDIVGIATDHCVRATALDALKEGFNVRVLTSMCSAVDFHAGDHALEELHE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCHHHHHHHHH
AGAILI
CCCCCC
>Mature Secondary Structure 
ARALILVDVQKDFCPGGSLATERGDEVAGKIGAYQLSHGSEYDVVVATQDWHIDPGEHF
CCEEEEEECCCCCCCCCCCCCCCCCHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCC
SETPDFKNSWPIHCVADSDGAAMHDRINTDSIDEFFRKGHYTAAYSGFEGTAVSEELLMS
CCCCCCCCCCCEEEEECCCCCEECCCCCCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHC
PWLKNKGVTDVDIVGIATDHCVRATALDALKEGFNVRVLTSMCSAVDFHAGDHALEELHE
CCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCHHHHHHHHH
AGAILI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]