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The map label for this gene is trmD [H]

Identifier: 145296014

GI number: 145296014

Start: 2126019

End: 2126888

Strand: Reverse

Name: trmD [H]

Synonym: cgR_1937

Alternate gene names: 145296014

Gene position: 2126888-2126019 (Counterclockwise)

Preceding gene: 145296016

Following gene: 145296013

Centisome position: 64.18

GC content: 60.69

Gene sequence:

>870_bases
GTGACTAGCTCCGAAAATCTTGATTCCCACCGTCTGCGGCTTGATGTCGTGACCATTTTCCCCGAATACCTGGATCCTCT
GCGCCATGCCCTGCTGGGTAAGGCGATTGAGGATGGCATTTTGGAAGTCGGTGTTCATGATCTTCGGAATTGGGCGACCG
GCGGACACAAAGCCGTCGACGATACCCCGTATGGCGGCGGTCCAGGCATGGTGATGAAGCCAGAGGTTTGGGGCCCAGCG
CTTGATGATGTAGCCGCAGGTCGGGTGAGCGGTGCGGAACTCGATTCGGCCTCGCCACACCTGAAAAGTGCGCGCCATGA
TGAGCTGGGTGGCGTCGAAAAGCGTGCTTATGTCGTGGAAGAAGACCGCGACCTGCCGCTGTTGCTGGTGCCCACCCCGG
CTGGCAAGCCGTTCACGCAGGCGGATGCGCAGGCGTGGTCCAACGAGGAGCACATTGTGTTCGCGTGCGGGCGCTACGAG
GGCATTGACCAGCGCGTTATTGATGATGCCGCCAACCGCTACCGCGTGCGCGAGGTATCGATCGGCGATTATGTGCTGAT
CGGCGGGGAAGTGGCAGTCCTGGTCATCGCGGAAGCCGTCGTGCGCCTGATCCCTGGCGTGCTCGGAAACCGTCGTAGCC
ACGAAGAAGACAGCTTCTCCGATGGCCTGCTCGAAGGCCCGTCGTACACAAAGCCGCGCACCTGGCGCGGACTTGACGTC
CCCGAAGTACTTTTCTCGGGCAACCACGCCAAGGTCGATCGCTGGCGACGCGATCAGGCGCTCCTACGCACCCAGGCAAT
TAGGCCTGAGCTTATCGACGCATCCCTCCTCGATTCCACCGACCTCAAAGTATTGGGACTGGACAAATGA

Upstream 100 bases:

>100_bases
AGCACCAAGAGCCCACCCACGCTGCCGATTGCTAAAGCTTGTCGCAAAAGTGTGTGAGGCTTCATGCCTCCAGCGTAGAC
CTCTTGGTAGATTGAAGTCC

Downstream 100 bases:

>100_bases
CAGAGACAACCCCTCAACCCCCAAAACCAGAGCCCGAACTCCCGAAATTTCTCAGCAACCCAGAACGCGCAGACATCATT
TTGTTCATCGCACTCATCGC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVDDTPYGGGPGMVMKPEVWGPA
LDDVAAGRVSGAELDSASPHLKSARHDELGGVEKRAYVVEEDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYE
GIDQRVIDDAANRYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDV
PEVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK

Sequences:

>Translated_289_residues
MTSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVDDTPYGGGPGMVMKPEVWGPA
LDDVAAGRVSGAELDSASPHLKSARHDELGGVEKRAYVVEEDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYE
GIDQRVIDDAANRYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDV
PEVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK
>Mature_288_residues
TSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVDDTPYGGGPGMVMKPEVWGPAL
DDVAAGRVSGAELDSASPHLKSARHDELGGVEKRAYVVEEDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYEG
IDQRVIDDAANRYRVREVSIGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDVP
EVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=274, Percent_Identity=34.3065693430657, Blast_Score=160, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 31720; Mature: 31589

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVD
CCCCCCCCCCEEEEEHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DTPYGGGPGMVMKPEVWGPALDDVAAGRVSGAELDSASPHLKSARHDELGGVEKRAYVVE
CCCCCCCCCCEECCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHCCCHHCCCCCCEEEEEE
EDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYEGIDQRVIDDAANRYRVREVS
CCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEC
IGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDV
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
PEVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
TSSENLDSHRLRLDVVTIFPEYLDPLRHALLGKAIEDGILEVGVHDLRNWATGGHKAVD
CCCCCCCCCEEEEEHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DTPYGGGPGMVMKPEVWGPALDDVAAGRVSGAELDSASPHLKSARHDELGGVEKRAYVVE
CCCCCCCCCCEECCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHCCCHHCCCCCCEEEEEE
EDRDLPLLLVPTPAGKPFTQADAQAWSNEEHIVFACGRYEGIDQRVIDDAANRYRVREVS
CCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEC
IGDYVLIGGEVAVLVIAEAVVRLIPGVLGNRRSHEEDSFSDGLLEGPSYTKPRTWRGLDV
CCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
PEVLFSGNHAKVDRWRRDQALLRTQAIRPELIDASLLDSTDLKVLGLDK
HHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]