Click here to switch to the map view.

The map label for this gene is rnhB

Identifier: 145295936

GI number: 145295936

Start: 2065548

End: 2066177

Strand: Reverse

Name: rnhB

Synonym: cgR_1861

Alternate gene names: 145295936

Gene position: 2066177-2065548 (Counterclockwise)

Preceding gene: 145295937

Following gene: 145295935

Centisome position: 62.34

GC content: 56.51

Gene sequence:

>630_bases
TTGTCACGCAACGGGCTAGGGCCCGTTGCAGGAGTAGACGAAGCTGGGCGCGGTGCCTGCTGCGGCCCCATCTCAATTGC
CGCATGCATCCTTCCGGACAAGCCCATCCAGGAGCTAGCCGCACTAACAGACTCCAAAAACCTCAGTGCGAGCACCCGCG
AAAAACTCATGCCACTGATCAAAAAACACGCACTCGCTTGGTCAGTTATCGTGATCTCCGCCCAAGATATCGACCGATTT
GGCATCCAACATGCAAACATTTCCGGCATGCGACGAGCCGTAGCTGCCCTAGACACCCAACCCGGCTACGTACTTACCGA
CGCCATGAAAGTCCCCGGCTTCACAGTCCCATACCTACCCATCATCGGCGGAGACGCCTCCGCCCGATGCATCGCCGCCG
CAAGTGTATTAGCCAAACAAACCCGCGACGACATCATGACCGACATGGCCAACGACTACCCGCACTACGGTCTCGAAATT
CACAAAGGCTACAGTACGAAGATCCACATGGATGCGGTGCGCCACCACGGCGCAAGTCCCGAGCACAGATATAGTTATGC
AAATGTGGCCAAGGCACACCAAGAATGGCTACACGCTGCAGATAATGACACGACGGAAGGTGGAGCATGA

Upstream 100 bases:

>100_bases
TCGGTGGCGTCGACGACCCTGCCATCCAGAGCTAGAAATTCGCTTTTCGACGCCTCCCTCAATGCGACGCTTAAAGCATC
TACGGACCTTTGAGGTCACC

Downstream 100 bases:

>100_bases
GCGCTGAAGAACTCGACAACTACGAAGCAGAGGTTGAACTCTCTCTTTACCGCGAATACCGCGACGTAGTCAGCCAGTTT
TCCTATGTTGTAGAAACTGA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSRNGLGPVAGVDEAGRGACCGPISIAACILPDKPIQELAALTDSKNLSASTREKLMPLIKKHALAWSVIVISAQDIDRF
GIQHANISGMRRAVAALDTQPGYVLTDAMKVPGFTVPYLPIIGGDASARCIAAASVLAKQTRDDIMTDMANDYPHYGLEI
HKGYSTKIHMDAVRHHGASPEHRYSYANVAKAHQEWLHAADNDTTEGGA

Sequences:

>Translated_209_residues
MSRNGLGPVAGVDEAGRGACCGPISIAACILPDKPIQELAALTDSKNLSASTREKLMPLIKKHALAWSVIVISAQDIDRF
GIQHANISGMRRAVAALDTQPGYVLTDAMKVPGFTVPYLPIIGGDASARCIAAASVLAKQTRDDIMTDMANDYPHYGLEI
HKGYSTKIHMDAVRHHGASPEHRYSYANVAKAHQEWLHAADNDTTEGGA
>Mature_208_residues
SRNGLGPVAGVDEAGRGACCGPISIAACILPDKPIQELAALTDSKNLSASTREKLMPLIKKHALAWSVIVISAQDIDRFG
IQHANISGMRRAVAALDTQPGYVLTDAMKVPGFTVPYLPIIGGDASARCIAAASVLAKQTRDDIMTDMANDYPHYGLEIH
KGYSTKIHMDAVRHHGASPEHRYSYANVAKAHQEWLHAADNDTTEGGA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=185, Percent_Identity=43.2432432432432, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CORGB (A4QF39)

Other databases:

- EMBL:   AP009044
- RefSeq:   YP_001138757.1
- STRING:   A4QF39
- GeneID:   4992374
- GenomeReviews:   AP009044_GR
- KEGG:   cgt:cgR_1861
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22327; Mature: 22195

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNGLGPVAGVDEAGRGACCGPISIAACILPDKPIQELAALTDSKNLSASTREKLMPLI
CCCCCCCCCCCCCCCCCCCCCCHHHHHEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KKHALAWSVIVISAQDIDRFGIQHANISGMRRAVAALDTQPGYVLTDAMKVPGFTVPYLP
HHHHHEEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEE
IIGGDASARCIAAASVLAKQTRDDIMTDMANDYPHYGLEIHKGYSTKIHMDAVRHHGASP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHCCCCC
EHRYSYANVAKAHQEWLHAADNDTTEGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRNGLGPVAGVDEAGRGACCGPISIAACILPDKPIQELAALTDSKNLSASTREKLMPLI
CCCCCCCCCCCCCCCCCCCCCHHHHHEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KKHALAWSVIVISAQDIDRFGIQHANISGMRRAVAALDTQPGYVLTDAMKVPGFTVPYLP
HHHHHEEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEE
IIGGDASARCIAAASVLAKQTRDDIMTDMANDYPHYGLEIHKGYSTKIHMDAVRHHGASP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHCCCCC
EHRYSYANVAKAHQEWLHAADNDTTEGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA