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The map label for this gene is liaS [H]

Identifier: 145295914

GI number: 145295914

Start: 2042856

End: 2044007

Strand: Reverse

Name: liaS [H]

Synonym: cgR_1839

Alternate gene names: 145295914

Gene position: 2044007-2042856 (Counterclockwise)

Preceding gene: 145295917

Following gene: 145295913

Centisome position: 61.67

GC content: 53.65

Gene sequence:

>1152_bases
TTGATCTATCGTGGTGGCGTGAAAACTAGCCAAGCGACCATCGCCCGAATTGAGAGAGTTCTCATTTGGGGATTGCATTT
ACTCATTGCCGTTTTGTTGGTGTTGGTGTGTTGGCGTGCCAGCCATTGGGGTGTGTGGGTGCTCGCTTTTGGCTATGGCG
TGGTTTATGTGGCGGGTGTGGTCCCGAATTCGCCGTTTAAGAATCACCCTATGGCGTGGTTTCTTGTGCTGAGTTTGTTG
TGGGCGAGCCTGATTTGGGATGGACCGGAGCCTGCGTATTTGGTGTTTCCGATGTTTTTCCTCGCAGTGTTGATCACGAC
ACCGCTGAAATCCGCGATCATCATTGCAATACTGACGGCGATCGCGGTGGTTACGTTGGCTATGCACCTGGGGTTTTCCG
TTGGCGTTGTCACCGGTCCGATCCTTGGCGCGTTGGTGGCGTGGGTAATGGGTACGTGTTTTCAGTTATTGGCACAAGCC
TTAAAGGAGCTTGTCGACGCACGTGCGTCGGCGATCCGGGCGTCGAAAAGCGCTGGCGAGCAGGCAGAACGAGCCCGCAT
AGCGGGCGAAATACATGACACTGTGGCGCAGGGGTTGTCCTCGATTCAGATGTTGTTGCATGCGGCGGAAAAACGGGTGG
ATGATCCGCAGGCGTTAAGCCATATACGGTTGGCCAGGCAAACGACAGCTGATAATTTGGCGGAGACCAGGCAGATCATT
GCTGCGCTGCAACCGACTCCACTCATTGGGGCGGATCTCCCGGTGGCGTTGGCCAGACTGTCGTCGACCACCCCGATGGG
ACAGAACATCACGTTTGAAGTCGACGGATCCCCACGGGTATTACCTGATGCGATGGAGGCAGAGATCGTACGAATTGCCC
AAACGCTGCTGGGAAATGTGGTGCGGCATGCACAGGCAGATTCTGCAAAAATGACCCTGACATATCAAGATGATCAAATA
CTTCTAGATGTCATCGATAATGGGCAGGGATTTGATGTGGCAGAGGTGATCCGTAAAAAATCCATCGGACTGCCCACAGC
GCAACGCCGGGCTGAAGGGCTGGGCGGAACAATAATTATTGAATCTACAATCGGATCGGGAACTGGAATTTCCGCCCGTT
TTCCCTATCCACAAAAGGACCAAGATAAGTGA

Upstream 100 bases:

>100_bases
TGAAACATGATTATTAGTTTCGTTGCGCGGGGGCATTTTTTAATCGGGCTCTGGTCGATTCTTGCAGTACGACCAAAGTC
GGATTCGCGTTCATACTTAG

Downstream 100 bases:

>100_bases
TCCGTATTCTGTTGGCTGATGATCATCCCGTTGTTCGCGCAGGCCTTGCCTCCTTGCTGGCGAGTGAAGATGATTTTGAG
ATAGTGGACATGGTGGGCAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MIYRGGVKTSQATIARIERVLIWGLHLLIAVLLVLVCWRASHWGVWVLAFGYGVVYVAGVVPNSPFKNHPMAWFLVLSLL
WASLIWDGPEPAYLVFPMFFLAVLITTPLKSAIIIAILTAIAVVTLAMHLGFSVGVVTGPILGALVAWVMGTCFQLLAQA
LKELVDARASAIRASKSAGEQAERARIAGEIHDTVAQGLSSIQMLLHAAEKRVDDPQALSHIRLARQTTADNLAETRQII
AALQPTPLIGADLPVALARLSSTTPMGQNITFEVDGSPRVLPDAMEAEIVRIAQTLLGNVVRHAQADSAKMTLTYQDDQI
LLDVIDNGQGFDVAEVIRKKSIGLPTAQRRAEGLGGTIIIESTIGSGTGISARFPYPQKDQDK

Sequences:

>Translated_383_residues
MIYRGGVKTSQATIARIERVLIWGLHLLIAVLLVLVCWRASHWGVWVLAFGYGVVYVAGVVPNSPFKNHPMAWFLVLSLL
WASLIWDGPEPAYLVFPMFFLAVLITTPLKSAIIIAILTAIAVVTLAMHLGFSVGVVTGPILGALVAWVMGTCFQLLAQA
LKELVDARASAIRASKSAGEQAERARIAGEIHDTVAQGLSSIQMLLHAAEKRVDDPQALSHIRLARQTTADNLAETRQII
AALQPTPLIGADLPVALARLSSTTPMGQNITFEVDGSPRVLPDAMEAEIVRIAQTLLGNVVRHAQADSAKMTLTYQDDQI
LLDVIDNGQGFDVAEVIRKKSIGLPTAQRRAEGLGGTIIIESTIGSGTGISARFPYPQKDQDK
>Mature_383_residues
MIYRGGVKTSQATIARIERVLIWGLHLLIAVLLVLVCWRASHWGVWVLAFGYGVVYVAGVVPNSPFKNHPMAWFLVLSLL
WASLIWDGPEPAYLVFPMFFLAVLITTPLKSAIIIAILTAIAVVTLAMHLGFSVGVVTGPILGALVAWVMGTCFQLLAQA
LKELVDARASAIRASKSAGEQAERARIAGEIHDTVAQGLSSIQMLLHAAEKRVDDPQALSHIRLARQTTADNLAETRQII
AALQPTPLIGADLPVALARLSSTTPMGQNITFEVDGSPRVLPDAMEAEIVRIAQTLLGNVVRHAQADSAKMTLTYQDDQI
LLDVIDNGQGFDVAEVIRKKSIGLPTAQRRAEGLGGTIIIESTIGSGTGISARFPYPQKDQDK

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=205, Percent_Identity=28.2926829268293, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41244; Mature: 41244

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYRGGVKTSQATIARIERVLIWGLHLLIAVLLVLVCWRASHWGVWVLAFGYGVVYVAGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEC
VPNSPFKNHPMAWFLVLSLLWASLIWDGPEPAYLVFPMFFLAVLITTPLKSAIIIAILTA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAVVTLAMHLGFSVGVVTGPILGALVAWVMGTCFQLLAQALKELVDARASAIRASKSAGE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QAERARIAGEIHDTVAQGLSSIQMLLHAAEKRVDDPQALSHIRLARQTTADNLAETRQII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALQPTPLIGADLPVALARLSSTTPMGQNITFEVDGSPRVLPDAMEAEIVRIAQTLLGNV
HHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VRHAQADSAKMTLTYQDDQILLDVIDNGQGFDVAEVIRKKSIGLPTAQRRAEGLGGTIII
HHHHCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCEEEE
ESTIGSGTGISARFPYPQKDQDK
EECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIYRGGVKTSQATIARIERVLIWGLHLLIAVLLVLVCWRASHWGVWVLAFGYGVVYVAGV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEC
VPNSPFKNHPMAWFLVLSLLWASLIWDGPEPAYLVFPMFFLAVLITTPLKSAIIIAILTA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAVVTLAMHLGFSVGVVTGPILGALVAWVMGTCFQLLAQALKELVDARASAIRASKSAGE
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
QAERARIAGEIHDTVAQGLSSIQMLLHAAEKRVDDPQALSHIRLARQTTADNLAETRQII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AALQPTPLIGADLPVALARLSSTTPMGQNITFEVDGSPRVLPDAMEAEIVRIAQTLLGNV
HHCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VRHAQADSAKMTLTYQDDQILLDVIDNGQGFDVAEVIRKKSIGLPTAQRRAEGLGGTIII
HHHHCCCCCEEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCEEEE
ESTIGSGTGISARFPYPQKDQDK
EECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]