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The map label for this gene is rpoA [H]

Identifier: 145294735

GI number: 145294735

Start: 741012

End: 742028

Strand: Direct

Name: rpoA [H]

Synonym: cgR_0683

Alternate gene names: 145294735

Gene position: 741012-742028 (Clockwise)

Preceding gene: 145294734

Following gene: 145294736

Centisome position: 22.36

GC content: 54.38

Gene sequence:

>1017_bases
ATGCTCATTTCACAGCGCCCAACCATCACCGAGGAATTTGTTGATAACGCACGTTCCCGGTTTGTCATCGAGCCACTGGA
GCCAGGTTTTGGCTACACCCTCGGTAACTCCCTACGCCGTACCCTGCTGTCCTCCATTCCTGGAGCAGCAGTAACCAGCG
TCAAGATTGACGGTGTACTCCACGAGTTCACCACCATCAGCGGTGTTAAGGAAGATGTCTCTGACATCATCTTGAACATC
AAGGGATTGGTTTTGTCTTCTGATTCCGATGAGCCAGTTGTTATGCAGCTGGTCAAGGAAGGCCCAGGAGTTGTAACTGC
AGGTGACATTCAGCCACCAGCAGGCGTGGAGATCCACAACCCGGATCTGCACATTGCAACCCTGAACGAGACCGCCAAGA
TTGAGATCGAGCTCATCGTCGAGCGTGGACGTGGTTACGTTCCCGCAACTGTTACTGCAACCGGTGGAGAGATCGGCCGC
ATTCCGGTCGATCAGATCTACTCCCCAGTACTGAAGGTCAGCTACAAGGTTGAAGCTACTCGTGTTGAGCAGCGCACCGA
CTTTGACAAGCTGGTCATCGACGTTGAGACCAAGAACTCTATTACCGCACGTGACGCCCTGGCGTCGGCAGGTAAGACCC
TGGTTGAGCTGTTCGGCCTCGCACGCGAGCTGAACATCGCAGCCGAGGGCATCGAGATCGGACCATCTCCTCAGGAGACC
GAGTACATCGCTGCCTACAGCATGCCAATCGAGGATCTGGACTTCTCTGTCCGTTCCTACAACTGCCTCAAGCGCGAAGA
CATCCACACCGTGGGTGAACTGGCAGAGCGCGCTGAGTCCGATTTGCTGGATATCCGCAACTTCGGACAGAAGTCGATCA
ACGAGGTAAAGATCAAGCTTGCTGGCCTGGGTCTGACCCTGAAGGATGCTCCTGAAGACTTCGATCCTTCAACTCTTGAA
GGTTATGACGCCGAAACTGGTGGCTACATCGATGTCGAGGCGGAAGATTCCGAGTAA

Upstream 100 bases:

>100_bases
GCCCCGGGTGCACAGGAATCTTTCGATTTTCCAAACATCCTTCATGGTTTTAAAAACCTTTACCGACGTCAAATAGCGGT
CGTCACTCAAGGAGAGTTCA

Downstream 100 bases:

>100_bases
GTCGCATGGTCCAATTCATGGGCCGCGCTAAATCAACGTACAAGGAGTACATCTAATGCCTACCCCTAAGAAGGGCGCCC
GCCTCGGCGGATCCGCAAGC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MLISQRPTITEEFVDNARSRFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSVKIDGVLHEFTTISGVKEDVSDIILNI
KGLVLSSDSDEPVVMQLVKEGPGVVTAGDIQPPAGVEIHNPDLHIATLNETAKIEIELIVERGRGYVPATVTATGGEIGR
IPVDQIYSPVLKVSYKVEATRVEQRTDFDKLVIDVETKNSITARDALASAGKTLVELFGLARELNIAAEGIEIGPSPQET
EYIAAYSMPIEDLDFSVRSYNCLKREDIHTVGELAERAESDLLDIRNFGQKSINEVKIKLAGLGLTLKDAPEDFDPSTLE
GYDAETGGYIDVEAEDSE

Sequences:

>Translated_338_residues
MLISQRPTITEEFVDNARSRFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSVKIDGVLHEFTTISGVKEDVSDIILNI
KGLVLSSDSDEPVVMQLVKEGPGVVTAGDIQPPAGVEIHNPDLHIATLNETAKIEIELIVERGRGYVPATVTATGGEIGR
IPVDQIYSPVLKVSYKVEATRVEQRTDFDKLVIDVETKNSITARDALASAGKTLVELFGLARELNIAAEGIEIGPSPQET
EYIAAYSMPIEDLDFSVRSYNCLKREDIHTVGELAERAESDLLDIRNFGQKSINEVKIKLAGLGLTLKDAPEDFDPSTLE
GYDAETGGYIDVEAEDSE
>Mature_338_residues
MLISQRPTITEEFVDNARSRFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSVKIDGVLHEFTTISGVKEDVSDIILNI
KGLVLSSDSDEPVVMQLVKEGPGVVTAGDIQPPAGVEIHNPDLHIATLNETAKIEIELIVERGRGYVPATVTATGGEIGR
IPVDQIYSPVLKVSYKVEATRVEQRTDFDKLVIDVETKNSITARDALASAGKTLVELFGLARELNIAAEGIEIGPSPQET
EYIAAYSMPIEDLDFSVRSYNCLKREDIHTVGELAERAESDLLDIRNFGQKSINEVKIKLAGLGLTLKDAPEDFDPSTLE
GYDAETGGYIDVEAEDSE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=315, Percent_Identity=44.7619047619048, Blast_Score=245, Evalue=3e-66,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 36672; Mature: 36672

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISQRPTITEEFVDNARSRFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSVKIDGVL
CCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
HEFTTISGVKEDVSDIILNIKGLVLSSDSDEPVVMQLVKEGPGVVTAGDIQPPAGVEIHN
HHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCEEEC
PDLHIATLNETAKIEIELIVERGRGYVPATVTATGGEIGRIPVDQIYSPVLKVSYKVEAT
CCEEEEECCCCEEEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEEEHH
RVEQRTDFDKLVIDVETKNSITARDALASAGKTLVELFGLARELNIAAEGIEIGPSPQET
HHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEECCEECCCCCCCC
EYIAAYSMPIEDLDFSVRSYNCLKREDIHTVGELAERAESDLLDIRNFGQKSINEVKIKL
CEEEEEECCHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
AGLGLTLKDAPEDFDPSTLEGYDAETGGYIDVEAEDSE
EECCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MLISQRPTITEEFVDNARSRFVIEPLEPGFGYTLGNSLRRTLLSSIPGAAVTSVKIDGVL
CCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
HEFTTISGVKEDVSDIILNIKGLVLSSDSDEPVVMQLVKEGPGVVTAGDIQPPAGVEIHN
HHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCEEEC
PDLHIATLNETAKIEIELIVERGRGYVPATVTATGGEIGRIPVDQIYSPVLKVSYKVEAT
CCEEEEECCCCEEEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEEEHH
RVEQRTDFDKLVIDVETKNSITARDALASAGKTLVELFGLARELNIAAEGIEIGPSPQET
HHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEECCEECCCCCCCC
EYIAAYSMPIEDLDFSVRSYNCLKREDIHTVGELAERAESDLLDIRNFGQKSINEVKIKL
CEEEEEECCHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
AGLGLTLKDAPEDFDPSTLEGYDAETGGYIDVEAEDSE
EECCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]