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The map label for this gene is paaI [H]

Identifier: 145294698

GI number: 145294698

Start: 705096

End: 705533

Strand: Direct

Name: paaI [H]

Synonym: cgR_0646

Alternate gene names: 145294698

Gene position: 705096-705533 (Clockwise)

Preceding gene: 145294694

Following gene: 145294699

Centisome position: 21.28

GC content: 49.77

Gene sequence:

>438_bases
ATGTCTGAAACTGCGGTTGATATTTCACAAATTGAGTCTGACCTGGAATTTACTCGAGCGATGTATGAAAACGATGGCTC
GTTGAAGGCCATGGGTATTTCCATTACCAAGTTGGAAACGGGTCATGTTGAGGGGGAATTTATTGTCCGTCCTGAGATGT
GCAATGGGCATAATTCCATCCAGGGTGGATTCTTGTTCACCTTCGCTGACGCACTTTTTGCTGGTGCGTGTAATTCCACC
AGGGGAGCGGTCACTGTTGCATCCCAGGTGCAGATCCATTTCATTGCGCCGGCTTTTGCGGGTGAAACACTTCGGGGTGT
GGCTATTGAGCGTCAGTCATGGGGCAGGAATGGTCTGTCTGATGTCACTGTTTTCCGTGGTGACAAGGTTATTGCAGAGT
TTCGGGGAATGTCTCGAACTGTCGGGAAGCGCGCCTAA

Upstream 100 bases:

>100_bases
CGAGTGAAATACTTCTAAGAAACTGTAGACTTTCTCAACCATAACCGGGAAAGGGCCTGGTTATGTAGAAATCACTCTAG
AGGGAAGTTGGGGATTTACC

Downstream 100 bases:

>100_bases
CGCTTTGCTGGGGTGTGGGTTTTCTACCCCCGGTTCACCCCTGTGAGCTGGTTTGTTGTAGAAATCTACCCCCGCATCAC
GTCCTGTCGATCGCGGAAAG

Product: hypothetical protein

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MSETAVDISQIESDLEFTRAMYENDGSLKAMGISITKLETGHVEGEFIVRPEMCNGHNSIQGGFLFTFADALFAGACNST
RGAVTVASQVQIHFIAPAFAGETLRGVAIERQSWGRNGLSDVTVFRGDKVIAEFRGMSRTVGKRA

Sequences:

>Translated_145_residues
MSETAVDISQIESDLEFTRAMYENDGSLKAMGISITKLETGHVEGEFIVRPEMCNGHNSIQGGFLFTFADALFAGACNST
RGAVTVASQVQIHFIAPAFAGETLRGVAIERQSWGRNGLSDVTVFRGDKVIAEFRGMSRTVGKRA
>Mature_144_residues
SETAVDISQIESDLEFTRAMYENDGSLKAMGISITKLETGHVEGEFIVRPEMCNGHNSIQGGFLFTFADALFAGACNSTR
GAVTVASQVQIHFIAPAFAGETLRGVAIERQSWGRNGLSDVTVFRGDKVIAEFRGMSRTVGKRA

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=125, Percent_Identity=42.4, Blast_Score=95, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 15666; Mature: 15535

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETAVDISQIESDLEFTRAMYENDGSLKAMGISITKLETGHVEGEFIVRPEMCNGHNSI
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCC
QGGFLFTFADALFAGACNSTRGAVTVASQVQIHFIAPAFAGETLRGVAIERQSWGRNGLS
CCCCHHHHHHHHHHCCCCCCCCEEEEECEEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
DVTVFRGDKVIAEFRGMSRTVGKRA
CEEEEECCCHHHHHCCCHHHCCCCC
>Mature Secondary Structure 
SETAVDISQIESDLEFTRAMYENDGSLKAMGISITKLETGHVEGEFIVRPEMCNGHNSI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCC
QGGFLFTFADALFAGACNSTRGAVTVASQVQIHFIAPAFAGETLRGVAIERQSWGRNGLS
CCCCHHHHHHHHHHCCCCCCCCEEEEECEEEEEEECCCCCCCCEEEEEEECCCCCCCCCC
DVTVFRGDKVIAEFRGMSRTVGKRA
CEEEEECCCHHHHHCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]