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The map label for this gene is nth [H]

Identifier: 145294424

GI number: 145294424

Start: 413109

End: 413891

Strand: Direct

Name: nth [H]

Synonym: cgR_0379

Alternate gene names: 145294424

Gene position: 413109-413891 (Clockwise)

Preceding gene: 145294421

Following gene: 145294425

Centisome position: 12.46

GC content: 55.3

Gene sequence:

>783_bases
ATGGGTTCCATTACTCCGCAGAAGCGGCCTCGCGTGGGGTCTCACATCGCGAACAAGGGTCAAGAGACTGATATCGGGCG
AAAACGCCGAGCTCGACGCATCAATCGCACACTCACCGTGGCGTATCCGGATGCTCACTGCGAATTAGATTTCACTAATC
CGCTAGAACTCACGGTCGCCACCATTTTGTCCGCCCAGTGCACGGACGTTCGCGTGAACCAGGTGACGCCCGCGTTGTTC
AAGCGCTATCCGACGGCCGCAGATTACGCCAACGCCGATCGCACGGAATTGGAGGAGTTCATCCGTCCGACAGGCTTTTA
CCGCAACAAGGCCACTTCTTTAATCGGCCTGGGTGAGGCACTAATTTCGCTTCACGACGGCCAGGTCCCCGGTACCCTTG
AGCAGCTAGTTGAGCTGCCGGGGGTCGGGCGGAAAACCGCCAACGTGGTGCTGGGAAATGCTTTCGGTGTTCCGGGAATC
ACGGTGGATACACACTTTGGCAGGTTGGTGCGTCGCCTGAAGCTCACTGATGAAGAAGATCCCGTCAAGGTGGAAAAAGT
GATGAACGAACTCATCGAAAAGCCTGAGTGGACCATGTTTTCACATAGGCTGATCTTCCACGGACGTAGGATATGTCATA
GTCGACGCGCCGCCTGTGGAGCCTGCATGCTGGCAGCTGATTGCCCATCCTTTGGTTTGGAGGGGCCGTCAGATCCATTT
GAGGCGCAAAAACTCATTAAAAGTGATGATAGGGAGCACCTGCTGAAAATGGCAGGAATGTAA

Upstream 100 bases:

>100_bases
CAAATGCAAAAAGCATCAGTAAGAGCGGGATGATCACCACAAGCAAACCTGTCATGGGAGTCATTCTAGTGATGAATCAA
ATTCACGCTAACTTAAGGGT

Downstream 100 bases:

>100_bases
AAAACAAATGACAAGCAGTGCAAAGTGGTCCATCGTTGGAGTTGTCGCCATCCTGGCTGTGATCGTTGCGTTAATCCCGC
AGCTTGTGGGCGGAGAAAGC

Product: hypothetical protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVATILSAQCTDVRVNQVTPALF
KRYPTAADYANADRTELEEFIRPTGFYRNKATSLIGLGEALISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGI
TVDTHFGRLVRRLKLTDEEDPVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPF
EAQKLIKSDDREHLLKMAGM

Sequences:

>Translated_260_residues
MGSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVATILSAQCTDVRVNQVTPALF
KRYPTAADYANADRTELEEFIRPTGFYRNKATSLIGLGEALISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGI
TVDTHFGRLVRRLKLTDEEDPVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPF
EAQKLIKSDDREHLLKMAGM
>Mature_259_residues
GSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVATILSAQCTDVRVNQVTPALFK
RYPTAADYANADRTELEEFIRPTGFYRNKATSLIGLGEALISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGIT
VDTHFGRLVRRLKLTDEEDPVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPFE
AQKLIKSDDREHLLKMAGM

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=193, Percent_Identity=29.0155440414508, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1787920, Length=186, Percent_Identity=38.1720430107527, Blast_Score=130, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17554540, Length=181, Percent_Identity=34.2541436464088, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324530, Length=219, Percent_Identity=25.5707762557078, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=146, Percent_Identity=30.8219178082192, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=30.7262569832402, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 28889; Mature: 28757

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVA
CCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCEEEECCCCCHHHHHH
TILSAQCTDVRVNQVTPALFKRYPTAADYANADRTELEEFIRPTGFYRNKATSLIGLGEA
HHHHHHCCCEEHHHCCHHHHHHCCCHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
LISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGITVDTHFGRLVRRLKLTDEED
HHHHCCCCCCHHHHHHHHCCCCCCCHHHEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCC
PVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPF
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
EAQKLIKSDDREHLLKMAGM
HHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
GSITPQKRPRVGSHIANKGQETDIGRKRRARRINRTLTVAYPDAHCELDFTNPLELTVA
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCEEEEEECCCEEEECCCCCHHHHHH
TILSAQCTDVRVNQVTPALFKRYPTAADYANADRTELEEFIRPTGFYRNKATSLIGLGEA
HHHHHHCCCEEHHHCCHHHHHHCCCHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
LISLHDGQVPGTLEQLVELPGVGRKTANVVLGNAFGVPGITVDTHFGRLVRRLKLTDEED
HHHHCCCCCCHHHHHHHHCCCCCCCHHHEEEECCCCCCCCEEHHHHHHHHHHHHCCCCCC
PVKVEKVMNELIEKPEWTMFSHRLIFHGRRICHSRRAACGACMLAADCPSFGLEGPSDPF
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
EAQKLIKSDDREHLLKMAGM
HHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]