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The map label for this gene is degA [H]

Identifier: 145294301

GI number: 145294301

Start: 278035

End: 279012

Strand: Direct

Name: degA [H]

Synonym: cgR_0257

Alternate gene names: 145294301

Gene position: 278035-279012 (Clockwise)

Preceding gene: 145294300

Following gene: 145294302

Centisome position: 8.39

GC content: 53.37

Gene sequence:

>978_bases
ATGAATCCGAGAGTCACCATCATCGATGTTGCCAAAGCAGCCGGTGTCTCCAAATCCACAGTCTCAGCAGCGCTAACCAA
CAACCCCACGGTTAATACCAAAACCCGGGACAAGGTTCTCGCCGTTGCCGCCGAGCTGGGATACCGCCCCAACAGGCTCG
CACGTTCACTGGTTCGAAAAAACAGTGGCGTTATTGGCGTCGTGGTGAGAAATCTACGAAACCCCTACTACGTTGACGTA
GTCGGGGGAATTGAAGATGCCTGCGAATCTCTTGGCCTGATGCCGATCATCAGCCACAGCCGCGACAAAGTAGAAGTGGC
ATCATCAGCCATCGACCAACTGTTGGAGCTGCAGGTTCAAGGAATCATCGTCATTTCCTCACGGATCAGCATGCAGACTG
TACGCACCGCTGCGAAAGCAGTACCCGTGGCTGTGATTGGCAGATTCAGCGATGACGACGGAGAAATCTTCAACATCGTC
GGTGATGATTTCGCAGGTGGCAGCATCGGCACCGCTCACTTGGCTGACCTCGGACACGAGCGCATCGCCTACGCCACCAC
CTCCGTTCGACTAGCCTCCACCTACAGGCAATCAGGCTACGAACAAGAGCTAAACAACCGAGGCCTACTACCGTTTACCG
TCAACGCCGACAACCCAAGAGACACCATCACAGCACTCCTGCAAAATGGTGTCACTGGAGTGGTCGCTCACAACGATGTC
ACCGCGGTAGCTCTTATAAATGCTGCGCATGAACAAGGCATTGAGCTACCCAAGGCATTGTCCATCGTGGGCTATGACGA
CACCTCACTGTGCGATTCCGCCAGACCCACTTTGACCAGCATTAATCAACCCCAAAAAGAAATGGGACGATTAGCCGTAG
AACACCTCTGGTCTGCCAGCCATGGCGAAAACGTGGGCCTTCAAAGAATCGAATTAAAGCCCTCCTTGCACCTGCGAAAA
TCAACAATCGCGATTTAA

Upstream 100 bases:

>100_bases
CCCTGTACCCATCGTTGACCTGGCTAAAGCCAGATGTCACCGTGGCAGATCCGCACGCACTTCTGTCTCTTCTGCAAACA
CAAATTGGTACAGTCAGGCT

Downstream 100 bases:

>100_bases
ACAAGAGTAAAATCTGACGTTTATGACTCCTGACCTTGCAGCCTTCCTGGACCAACTTTATGCCGACGGACGAGACTTCG
ATGCAGGACAACCTGATCGG

Product: hypothetical protein

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNPRVTIIDVAKAAGVSKSTVSAALTNNPTVNTKTRDKVLAVAAELGYRPNRLARSLVRKNSGVIGVVVRNLRNPYYVDV
VGGIEDACESLGLMPIISHSRDKVEVASSAIDQLLELQVQGIIVISSRISMQTVRTAAKAVPVAVIGRFSDDDGEIFNIV
GDDFAGGSIGTAHLADLGHERIAYATTSVRLASTYRQSGYEQELNNRGLLPFTVNADNPRDTITALLQNGVTGVVAHNDV
TAVALINAAHEQGIELPKALSIVGYDDTSLCDSARPTLTSINQPQKEMGRLAVEHLWSASHGENVGLQRIELKPSLHLRK
STIAI

Sequences:

>Translated_325_residues
MNPRVTIIDVAKAAGVSKSTVSAALTNNPTVNTKTRDKVLAVAAELGYRPNRLARSLVRKNSGVIGVVVRNLRNPYYVDV
VGGIEDACESLGLMPIISHSRDKVEVASSAIDQLLELQVQGIIVISSRISMQTVRTAAKAVPVAVIGRFSDDDGEIFNIV
GDDFAGGSIGTAHLADLGHERIAYATTSVRLASTYRQSGYEQELNNRGLLPFTVNADNPRDTITALLQNGVTGVVAHNDV
TAVALINAAHEQGIELPKALSIVGYDDTSLCDSARPTLTSINQPQKEMGRLAVEHLWSASHGENVGLQRIELKPSLHLRK
STIAI
>Mature_325_residues
MNPRVTIIDVAKAAGVSKSTVSAALTNNPTVNTKTRDKVLAVAAELGYRPNRLARSLVRKNSGVIGVVVRNLRNPYYVDV
VGGIEDACESLGLMPIISHSRDKVEVASSAIDQLLELQVQGIIVISSRISMQTVRTAAKAVPVAVIGRFSDDDGEIFNIV
GDDFAGGSIGTAHLADLGHERIAYATTSVRLASTYRQSGYEQELNNRGLLPFTVNADNPRDTITALLQNGVTGVVAHNDV
TAVALINAAHEQGIELPKALSIVGYDDTSLCDSARPTLTSINQPQKEMGRLAVEHLWSASHGENVGLQRIELKPSLHLRK
STIAI

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=330, Percent_Identity=28.7878787878788, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=29.5731707317073, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786540, Length=337, Percent_Identity=31.4540059347181, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789068, Length=315, Percent_Identity=29.8412698412698, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI48994940, Length=324, Percent_Identity=27.7777777777778, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788474, Length=302, Percent_Identity=29.8013245033113, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787906, Length=308, Percent_Identity=31.1688311688312, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787580, Length=316, Percent_Identity=28.4810126582279, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=28.0276816608997, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787948, Length=329, Percent_Identity=26.1398176291793, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=24.1157556270096, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786268, Length=311, Percent_Identity=24.1157556270096, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790689, Length=304, Percent_Identity=23.6842105263158, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34745; Mature: 34745

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRVTIIDVAKAAGVSKSTVSAALTNNPTVNTKTRDKVLAVAAELGYRPNRLARSLVRK
CCCCEEEEEEHHHCCCCHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
NSGVIGVVVRNLRNPYYVDVVGGIEDACESLGLMPIISHSRDKVEVASSAIDQLLELQVQ
CCCEEEEEHHHCCCCEEEEEECCHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHC
GIIVISSRISMQTVRTAAKAVPVAVIGRFSDDDGEIFNIVGDDFAGGSIGTAHLADLGHE
EEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHCCCC
RIAYATTSVRLASTYRQSGYEQELNNRGLLPFTVNADNPRDTITALLQNGVTGVVAHNDV
CEEEEEHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCE
TAVALINAAHEQGIELPKALSIVGYDDTSLCDSARPTLTSINQPQKEMGRLAVEHLWSAS
EEEEEEHHHHHCCCCCCHHEEEEECCCHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHCCC
HGENVGLQRIELKPSLHLRKSTIAI
CCCCCCCEEEEECCCCEECHHCCCC
>Mature Secondary Structure
MNPRVTIIDVAKAAGVSKSTVSAALTNNPTVNTKTRDKVLAVAAELGYRPNRLARSLVRK
CCCCEEEEEEHHHCCCCHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
NSGVIGVVVRNLRNPYYVDVVGGIEDACESLGLMPIISHSRDKVEVASSAIDQLLELQVQ
CCCEEEEEHHHCCCCEEEEEECCHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHC
GIIVISSRISMQTVRTAAKAVPVAVIGRFSDDDGEIFNIVGDDFAGGSIGTAHLADLGHE
EEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEECCCCCCCCCCHHHHHHCCCC
RIAYATTSVRLASTYRQSGYEQELNNRGLLPFTVNADNPRDTITALLQNGVTGVVAHNDV
CEEEEEHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCE
TAVALINAAHEQGIELPKALSIVGYDDTSLCDSARPTLTSINQPQKEMGRLAVEHLWSAS
EEEEEEHHHHHCCCCCCHHEEEEECCCHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHCCC
HGENVGLQRIELKPSLHLRKSTIAI
CCCCCCCEEEEECCCCEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]