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The map label for this gene is rsmE [H]

Identifier: 229577456

GI number: 229577456

Start: 2544289

End: 2545065

Strand: Reverse

Name: rsmE [H]

Synonym: GTNG_2437

Alternate gene names: 229577456

Gene position: 2545065-2544289 (Counterclockwise)

Preceding gene: 138896075

Following gene: 138896073

Centisome position: 71.69

GC content: 56.63

Gene sequence:

>777_bases
GTGCAGCGTTATTTCGTTTCCGACCGGGAGCGCCAAGGCGAAAACGTCGTTATCAGCGGCGATGACGCCCATCATATTTC
CCGCGTTATGCGCATGAAGCCGGGAGACGCCATTGCCTGCGTATTTCCGGATGGACGCACGGCCGTGTGCCAAATTGAAC
AAATTGCCAATGAGCAAGTATGTGCCCGTATTGTACAATGGAAGGAAGAAGGGCGCGAATTGCCGGTGCGCATTTATATC
GCCCAAGGGCTGCCAAAAGGAGAAAAATGGGAGCTCGTCATCCAAAAAGGAACGGAGCTCGGCGCTGCCGGCTTTCTTCC
ATTTGTGGCCGCTCGTTCGGTCGTTAAATGGGATGCCAAAAAAGCAGAGAAAAAAGTGGAGCGGTGGAAAAAAATTGCGA
AAGAGGCGGCCGAGCAGGCCGAACGAACGTTGTTGCCCATCGTGCGGGCGCCGCTTTCGCTTAAGGAACTGATCGCTTTC
GGGGAAACGGTTGATAGCCGGCTGTTTGCCTATGAAGAAGAAGCGCGCGGAGGGCGGCATGCCTCACTTCCGGCGCTCCT
TGCCGACCTTCAGCCCGGCAGCTCGCTTTTAGCCGTCTTTGGGCCGGAAGGCGGGTTCAGCCGTGAGGAGGCGGAACAGC
TCAAGCAAAGCGGCTTTTTACCGTGCAGCCTCGGCCCGCGTATTTTGCGGACGGAAACGGCGCCGCTCTACTTGCTTGCC
GCCGCTTCGTACGAATGGGAGCTTTGTGAATCAGGCTCTAAGAGAGAGCGAGAGTAA

Upstream 100 bases:

>100_bases
CGGACTGCTTGCTGCTGGATTTTTCATTGAAGAAGTCAATGTGATGGAAGACTGGGTGGCGTTTGTCGCCATCAAACCGT
AAGCAAAGAAGGGATGTTTG

Downstream 100 bases:

>100_bases
CGTAGCCGATTTGGAGTTTATCATAAAACATCTAGCCCTCGCTGGCGATCAGCGGTGGTGCAGAGTGAGGTGAAGAATAT
GCCAACAGTGGCTTTCCATA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MQRYFVSDRERQGENVVISGDDAHHISRVMRMKPGDAIACVFPDGRTAVCQIEQIANEQVCARIVQWKEEGRELPVRIYI
AQGLPKGEKWELVIQKGTELGAAGFLPFVAARSVVKWDAKKAEKKVERWKKIAKEAAEQAERTLLPIVRAPLSLKELIAF
GETVDSRLFAYEEEARGGRHASLPALLADLQPGSSLLAVFGPEGGFSREEAEQLKQSGFLPCSLGPRILRTETAPLYLLA
AASYEWELCESGSKRERE

Sequences:

>Translated_258_residues
MQRYFVSDRERQGENVVISGDDAHHISRVMRMKPGDAIACVFPDGRTAVCQIEQIANEQVCARIVQWKEEGRELPVRIYI
AQGLPKGEKWELVIQKGTELGAAGFLPFVAARSVVKWDAKKAEKKVERWKKIAKEAAEQAERTLLPIVRAPLSLKELIAF
GETVDSRLFAYEEEARGGRHASLPALLADLQPGSSLLAVFGPEGGFSREEAEQLKQSGFLPCSLGPRILRTETAPLYLLA
AASYEWELCESGSKRERE
>Mature_258_residues
MQRYFVSDRERQGENVVISGDDAHHISRVMRMKPGDAIACVFPDGRTAVCQIEQIANEQVCARIVQWKEEGRELPVRIYI
AQGLPKGEKWELVIQKGTELGAAGFLPFVAARSVVKWDAKKAEKKVERWKKIAKEAAEQAERTLLPIVRAPLSLKELIAF
GETVDSRLFAYEEEARGGRHASLPALLADLQPGSSLLAVFGPEGGFSREEAEQLKQSGFLPCSLGPRILRTETAPLYLLA
AASYEWELCESGSKRERE

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=227, Percent_Identity=31.2775330396476, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28735; Mature: 28735

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRYFVSDRERQGENVVISGDDAHHISRVMRMKPGDAIACVFPDGRTAVCQIEQIANEQV
CCCCCCCCHHCCCCEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHH
CARIVQWKEEGRELPVRIYIAQGLPKGEKWELVIQKGTELGAAGFLPFVAARSVVKWDAK
HHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
KAEKKVERWKKIAKEAAEQAERTLLPIVRAPLSLKELIAFGETVDSRLFAYEEEARGGRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC
ASLPALLADLQPGSSLLAVFGPEGGFSREEAEQLKQSGFLPCSLGPRILRTETAPLYLLA
CCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEE
AASYEWELCESGSKRERE
ECCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MQRYFVSDRERQGENVVISGDDAHHISRVMRMKPGDAIACVFPDGRTAVCQIEQIANEQV
CCCCCCCCHHCCCCEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHH
CARIVQWKEEGRELPVRIYIAQGLPKGEKWELVIQKGTELGAAGFLPFVAARSVVKWDAK
HHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
KAEKKVERWKKIAKEAAEQAERTLLPIVRAPLSLKELIAFGETVDSRLFAYEEEARGGRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCC
ASLPALLADLQPGSSLLAVFGPEGGFSREEAEQLKQSGFLPCSLGPRILRTETAPLYLLA
CCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEE
AASYEWELCESGSKRERE
ECCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]