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The map label for this gene is speE1 [H]

Identifier: 138896979

GI number: 138896979

Start: 3458414

End: 3459241

Strand: Reverse

Name: speE1 [H]

Synonym: GTNG_3350

Alternate gene names: 138896979

Gene position: 3459241-3458414 (Counterclockwise)

Preceding gene: 138896981

Following gene: 138896978

Centisome position: 97.43

GC content: 47.46

Gene sequence:

>828_bases
ATGGAACTATGGTTTACTGAAAAGCAGACAGAACATTTTGGCATCACTGCACGTATTATCCGCACTTTACATACAGAACA
AACGCCGTTTCAAAAACTTGATATGGTCGAAACGGCAGAGTTCGGTAATATGCTCATTTTAGACGGCATGGTGATGACGA
CGCAAAAAGATGAATTTGTCTACCATGAGATGGTTGCTCATGTGCCGTTGTTTACCCATCCAAATCCGGAAAACGTGCTT
GTCGTTGGCGGTGGCGACGGCGGCGTAATCCGCGAAGTGTTGAAACACCCGAGCGTCAAAAAAGCGACGCTTGTCGAAAT
TGACGGCAAAGTGATCGAGTGCTCGAAAAAGTATTTGCCGGAAATCGCTGGCCAACTTGACGATCCGCGCGTCGAAGTGA
AAGTCGATGACGGGTTTATGCATATCGCCCAAAGTGAAAACGAGTATGACGTCATTATGGTTGACTCGACCGAACCGGTC
GGACCGGCGGTGAACTTGTTTACAAAAGGGTTTTACGCCGGCATTGCGAAAGCGTTGAAAGAAGACGGTATTTTCGTCGC
CCAAACGGACAACCCTTGGTTTAAAGCGGATCTGATTCGCAATGTTTACCGTGATGTGAAGGAAATTTTCCCGGTCACGC
GCCTATATACGGCGAACATTCCAACCTACCCAAGCGGGCTGTGGACGTTTACGCTCGGCTCGAAAAAATACGATCCGCTC
GCGGTAAGCGATGACCGGTTCCACGACATCGATACAAAATATTACACAAAAGAACTGCATAAAGCATGCTTCGTATTACC
GAAATTTGTCGCGGACTTGGTGAAATAA

Upstream 100 bases:

>100_bases
GCATTTTTGCTAGATTGCTCTATACTTTTTCATGTTTAGTTTTTCGTTGGTGAGGGAAACTTTGATAGTAAGCAACGCTC
GGAGAAAGGATGTTACCGAC

Downstream 100 bases:

>100_bases
GGGGAGGGGCAAATTGATGCGGTTTGATGAGGCCTATTCTGGCAACGTGTTTATTGGTAGCCACCCGAACTTTGAAGAAA
GCGAGGCAGTCATTTACGGG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MELWFTEKQTEHFGITARIIRTLHTEQTPFQKLDMVETAEFGNMLILDGMVMTTQKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIECSKKYLPEIAGQLDDPRVEVKVDDGFMHIAQSENEYDVIMVDSTEPV
GPAVNLFTKGFYAGIAKALKEDGIFVAQTDNPWFKADLIRNVYRDVKEIFPVTRLYTANIPTYPSGLWTFTLGSKKYDPL
AVSDDRFHDIDTKYYTKELHKACFVLPKFVADLVK

Sequences:

>Translated_275_residues
MELWFTEKQTEHFGITARIIRTLHTEQTPFQKLDMVETAEFGNMLILDGMVMTTQKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIECSKKYLPEIAGQLDDPRVEVKVDDGFMHIAQSENEYDVIMVDSTEPV
GPAVNLFTKGFYAGIAKALKEDGIFVAQTDNPWFKADLIRNVYRDVKEIFPVTRLYTANIPTYPSGLWTFTLGSKKYDPL
AVSDDRFHDIDTKYYTKELHKACFVLPKFVADLVK
>Mature_275_residues
MELWFTEKQTEHFGITARIIRTLHTEQTPFQKLDMVETAEFGNMLILDGMVMTTQKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIECSKKYLPEIAGQLDDPRVEVKVDDGFMHIAQSENEYDVIMVDSTEPV
GPAVNLFTKGFYAGIAKALKEDGIFVAQTDNPWFKADLIRNVYRDVKEIFPVTRLYTANIPTYPSGLWTFTLGSKKYDPL
AVSDDRFHDIDTKYYTKELHKACFVLPKFVADLVK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=277, Percent_Identity=39.3501805054152, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1786312, Length=275, Percent_Identity=37.4545454545455, Blast_Score=205, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI25154106, Length=268, Percent_Identity=38.0597014925373, Blast_Score=200, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI212645616, Length=222, Percent_Identity=42.7927927927928, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6325326, Length=277, Percent_Identity=39.3501805054152, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323175, Length=286, Percent_Identity=33.9160839160839, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24645443, Length=274, Percent_Identity=39.4160583941606, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI21355941, Length=211, Percent_Identity=40.2843601895735, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31191; Mature: 31191

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELWFTEKQTEHFGITARIIRTLHTEQTPFQKLDMVETAEFGNMLILDGMVMTTQKDEFV
CCCEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCEEEECCCHHHH
YHEMVAHVPLFTHPNPENVLVVGGGDGGVIREVLKHPSVKKATLVEIDGKVIECSKKYLP
HHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCEEEEHHHHHHH
EIAGQLDDPRVEVKVDDGFMHIAQSENEYDVIMVDSTEPVGPAVNLFTKGFYAGIAKALK
HHHCCCCCCEEEEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EDGIFVAQTDNPWFKADLIRNVYRDVKEIFPVTRLYTANIPTYPSGLWTFTLGSKKYDPL
HCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCEEEEEECCCCCCCE
AVSDDRFHDIDTKYYTKELHKACFVLPKFVADLVK
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELWFTEKQTEHFGITARIIRTLHTEQTPFQKLDMVETAEFGNMLILDGMVMTTQKDEFV
CCCEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCEEEECCCHHHH
YHEMVAHVPLFTHPNPENVLVVGGGDGGVIREVLKHPSVKKATLVEIDGKVIECSKKYLP
HHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCEEEEHHHHHHH
EIAGQLDDPRVEVKVDDGFMHIAQSENEYDVIMVDSTEPVGPAVNLFTKGFYAGIAKALK
HHHCCCCCCEEEEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EDGIFVAQTDNPWFKADLIRNVYRDVKEIFPVTRLYTANIPTYPSGLWTFTLGSKKYDPL
HCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCEEEEEECCCCCCCE
AVSDDRFHDIDTKYYTKELHKACFVLPKFVADLVK
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]