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The map label for this gene is potD-A [H]

Identifier: 138896862

GI number: 138896862

Start: 3336523

End: 3337614

Strand: Reverse

Name: potD-A [H]

Synonym: GTNG_3227

Alternate gene names: 138896862

Gene position: 3337614-3336523 (Counterclockwise)

Preceding gene: 138896863

Following gene: 138896861

Centisome position: 94.01

GC content: 37.73

Gene sequence:

>1092_bases
ATGATGTTGAAAAGTAAAACAGTTTTAACTGTGTTGATCTGTTTGCTATTTACTATGATTGTGGGATGTGGTAACCAAAC
TGCTAGCTCACCGAATCAAAGTTCGAAAAGTGGGGGAGAAAAGACGTTAGTTTTGGCTGCCTATGGTGGAAGTTATGAAA
AAAAGATGAAGGAAACGATCATTCCTAAGTTTGAGAAAGAGTTTGGGGTTAAGGTTAAGTATATAACTGGGAGCTCCATC
GACACATTATCAAAACTTCAGGCCCAAAAAGATAATCCTCAAATTGATGTAGCCTTTTTAGACGACGGACCTCAAGCACA
GGCAAAAGCTTTTGGATTACTTGCCCCGTTAGATGAAAAAATAGTCACCAACCTAAATAATGTATACGACATTGCAAAAG
ATAAGGAGAATATTGGAGTTGGCTTTGGTATTGTTGTGACAGGATTAGCCTATAACAAAGATTATTTTGCGCAGAATGGT
TGGAAACCGTTAACATCTTGGAATGATCTGGAGGATCCTAAGTTTAAAGGAAAACTCGTATTACCATCTATCTCAAACAC
TTATGGAGTTCATCTGCTTCTAATGGCTGCTTTTGCGAATGGTGGGAACGAGAAAAATATTGAACCTGGATTTGAAAAGC
TAAAGAAGATTGCTGAAAACGCTGTAACATTTGATAAAACTGCTGACGTGTCCAATTACTTTTTGCAAGGTCAAACAGTA
GCCAGTGCATGGGGAAGCAGCAGAGTCTATACACTGCAAGATTCAGGATTTCCAATTGAATTTGTCATTCCAAGAGAAGG
TGCTCCTGCTTTAATGACAACAGCAAATGTGGTGAAAAATGCACCAAATGCGGAACTTGCACAGAAATTTGTCAATTTTA
TTTTGAGTGAGGAAGTACAAAAGGAATTCGCCCATTCTTTGTTTGATGGTCCTGTTAATAAAAATGTAAAACTAGATGGA
GATATTGCTAAAAAAGTGGTTTATGGGGAAGAGCAGGTTAAAAAGTTAATCAAAGTGGATTGGGAATACGTAAATCAAAA
AAGAGCTGAATGGACGGAAAGAGCAAACAAGGAAATTGAAGTAACCCGCTAA

Upstream 100 bases:

>100_bases
AGATTCCTATAGGGAGGTGATTAAAAAATCATCCTAAAATTAGTGAATTAATCGGCGGTGTTAAGGCTATCTGAAAAAAT
GATATCGGGGTGGGGAGAAA

Downstream 100 bases:

>100_bases
AGAGGGAGGGAAGGTAAATGGGTTATTTGTGTCTCGAGAATGTTGTTAAGCGATTTGATAAAACAGAAGTGGTAAAAAAT
TTGAATCTAGAAATTCATCA

Product: ABC transporter substrate-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MMLKSKTVLTVLICLLFTMIVGCGNQTASSPNQSSKSGGEKTLVLAAYGGSYEKKMKETIIPKFEKEFGVKVKYITGSSI
DTLSKLQAQKDNPQIDVAFLDDGPQAQAKAFGLLAPLDEKIVTNLNNVYDIAKDKENIGVGFGIVVTGLAYNKDYFAQNG
WKPLTSWNDLEDPKFKGKLVLPSISNTYGVHLLLMAAFANGGNEKNIEPGFEKLKKIAENAVTFDKTADVSNYFLQGQTV
ASAWGSSRVYTLQDSGFPIEFVIPREGAPALMTTANVVKNAPNAELAQKFVNFILSEEVQKEFAHSLFDGPVNKNVKLDG
DIAKKVVYGEEQVKKLIKVDWEYVNQKRAEWTERANKEIEVTR

Sequences:

>Translated_363_residues
MMLKSKTVLTVLICLLFTMIVGCGNQTASSPNQSSKSGGEKTLVLAAYGGSYEKKMKETIIPKFEKEFGVKVKYITGSSI
DTLSKLQAQKDNPQIDVAFLDDGPQAQAKAFGLLAPLDEKIVTNLNNVYDIAKDKENIGVGFGIVVTGLAYNKDYFAQNG
WKPLTSWNDLEDPKFKGKLVLPSISNTYGVHLLLMAAFANGGNEKNIEPGFEKLKKIAENAVTFDKTADVSNYFLQGQTV
ASAWGSSRVYTLQDSGFPIEFVIPREGAPALMTTANVVKNAPNAELAQKFVNFILSEEVQKEFAHSLFDGPVNKNVKLDG
DIAKKVVYGEEQVKKLIKVDWEYVNQKRAEWTERANKEIEVTR
>Mature_363_residues
MMLKSKTVLTVLICLLFTMIVGCGNQTASSPNQSSKSGGEKTLVLAAYGGSYEKKMKETIIPKFEKEFGVKVKYITGSSI
DTLSKLQAQKDNPQIDVAFLDDGPQAQAKAFGLLAPLDEKIVTNLNNVYDIAKDKENIGVGFGIVVTGLAYNKDYFAQNG
WKPLTSWNDLEDPKFKGKLVLPSISNTYGVHLLLMAAFANGGNEKNIEPGFEKLKKIAENAVTFDKTADVSNYFLQGQTV
ASAWGSSRVYTLQDSGFPIEFVIPREGAPALMTTANVVKNAPNAELAQKFVNFILSEEVQKEFAHSLFDGPVNKNVKLDG
DIAKKVVYGEEQVKKLIKVDWEYVNQKRAEWTERANKEIEVTR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=256, Percent_Identity=26.953125, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40041; Mature: 40041

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLKSKTVLTVLICLLFTMIVGCGNQTASSPNQSSKSGGEKTLVLAAYGGSYEKKMKETI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHH
IPKFEKEFGVKVKYITGSSIDTLSKLQAQKDNPQIDVAFLDDGPQAQAKAFGLLAPLDEK
CCCHHHHHCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHH
IVTNLNNVYDIAKDKENIGVGFGIVVTGLAYNKDYFAQNGWKPLTSWNDLEDPKFKGKLV
HHHHHHHHHHHHCCCCCCCCCHHHHHCCEECCCCHHHCCCCCCCCCCCCCCCCCCCCEEE
LPSISNTYGVHLLLMAAFANGGNEKNIEPGFEKLKKIAENAVTFDKTADVSNYFLQGQTV
ECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHH
ASAWGSSRVYTLQDSGFPIEFVIPREGAPALMTTANVVKNAPNAELAQKFVNFILSEEVQ
HHHCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KEFAHSLFDGPVNKNVKLDGDIAKKVVYGEEQVKKLIKVDWEYVNQKRAEWTERANKEIE
HHHHHHHHCCCCCCCEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VTR
ECC
>Mature Secondary Structure
MMLKSKTVLTVLICLLFTMIVGCGNQTASSPNQSSKSGGEKTLVLAAYGGSYEKKMKETI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHH
IPKFEKEFGVKVKYITGSSIDTLSKLQAQKDNPQIDVAFLDDGPQAQAKAFGLLAPLDEK
CCCHHHHHCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHH
IVTNLNNVYDIAKDKENIGVGFGIVVTGLAYNKDYFAQNGWKPLTSWNDLEDPKFKGKLV
HHHHHHHHHHHHCCCCCCCCCHHHHHCCEECCCCHHHCCCCCCCCCCCCCCCCCCCCEEE
LPSISNTYGVHLLLMAAFANGGNEKNIEPGFEKLKKIAENAVTFDKTADVSNYFLQGQTV
ECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHH
ASAWGSSRVYTLQDSGFPIEFVIPREGAPALMTTANVVKNAPNAELAQKFVNFILSEEVQ
HHHCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KEFAHSLFDGPVNKNVKLDGDIAKKVVYGEEQVKKLIKVDWEYVNQKRAEWTERANKEIE
HHHHHHHHCCCCCCCEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VTR
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]