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The map label for this gene is yrvJ [H]

Identifier: 138896826

GI number: 138896826

Start: 3293466

End: 3294815

Strand: Reverse

Name: yrvJ [H]

Synonym: GTNG_3189

Alternate gene names: 138896826

Gene position: 3294815-3293466 (Counterclockwise)

Preceding gene: 138896827

Following gene: 138896825

Centisome position: 92.8

GC content: 53.11

Gene sequence:

>1350_bases
ATGAGGCCAAGAAGATGGTTCGCCATATTGGTTTGTTTGTGTTGTTTGGTTACAGCTGCCTGGCCGGCAGAGGTGAAAGG
GGAAAATAAGAAGAAGGAACGATTGGCTGTCGTGACGGTTGATCGGGTGAACGTCCGCCAAGGGCCGGGTGTGCCGTACC
GTCCGTTGGCGAACGTTCACCGTGGTGAAACGTATCGGTTGATCGATATAAAAGACGGCTGGTTGAAAATAGAATGGAAG
AAGAATAAAATCGGATGGATTGCCGCATCGTATGCCGCTCCTGTGCGCGAAATGGAAATCGTGCAAGAAGATCGGCTTCG
TCTTCGGCAAGAGCCGGGGCTAGATGGGCGTATTATTGGCCATCTGGCACAGGGGGATCAAGTCATTGTCATCAAAGAAA
AGGGAGAATGGAAACAAATCGTCACGAAAAAGGCTGTTGGTTGGGTGGCGGCATCGTATTTGGCAGATGCCGAATCTCCA
ACTGGTTCACGGCAGACAGGAGTCGTGACGGCTGATTCACTGAACGTGCGGGTTGCGCCGTCATTGGATGCGGAGCGGAT
CGGACGGCTTTTGCACGGTGAGCGCGTTGAGATTGTCGAAACAAAGCGAGATTGGTATAAGATCGTTACCCGTTCCGGGC
TAGGTGGTTGGGTGGCGGCGGAGTACATCGAAATGAAAAATGGACAGGCCGTCGGGAATCCGGAAGAGGCAGTGGCTGAA
TCGGCTTCGCCTGCTGCTGTTGACCTTGTCACCATTCAGGGAAATGGGCCGCGTCAATATGTAAAAAAATGGACGCGCAC
ACCTGTTCAAGTGCTTGCCGGAAAAACGATCGTGCTTGACGCCGGCCATGGCGGCAAAGACGGGGGAGCGGAAAGCGTCA
ATGGCGTAACGGAAAAAACATTGACAATGGAAACGGCCGAGCGGTTGAAAGAAAAGCTCGAAACATACGGAGCGCGCGTG
GTGTTGACACGTGTCAATGATGATTACGTGCCGCTGTCGGCACGCGTGGCCACCGCCCGTTTGTACCAGGCGGATGCGTT
TATTAGTTTACATTACGACAGCGCGGAGGATGAAGATGCTTCTGGCATCACCGCCTACTATTACGATCGGTTCGCCGACT
ACGGGTTGGCGCAATCGTTCCAAGGGCCGTTCTCAAAGCTCTCTGCTTTGCCATTTCGCGGCCTCGCCTTTGGCAACTAT
TACGTACTGCGGGAAAATGAACGACCGTCTGTGCTGCTTGAGCTTGGCTATTTAAGCAATCGGTCGGATGCCGAGGTTGT
GGCGACAGACAGCTATCAAGAAACAGTGACGACGGCTATCGTGAACGCCGTGCGCCATTATTTTCAATAA

Upstream 100 bases:

>100_bases
CGCTCGTTCTGTCGAGCGGTTTTCCCGACCGACAGACAGGAAATCGACTCTTACGTGCGAACCATATAGTAGAACGAGAG
ACATCTAGGAGGGAAGGTGC

Downstream 100 bases:

>100_bases
CAAACCGGTCATTTTGCCAAAAATGGGCAACGGATCTGCGGCATACAGGCCGCACGACAAAAAGCGTCCCGATTTTTCGG
GGCGCTTTTTTACCATTTCA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MRPRRWFAILVCLCCLVTAAWPAEVKGENKKKERLAVVTVDRVNVRQGPGVPYRPLANVHRGETYRLIDIKDGWLKIEWK
KNKIGWIAASYAAPVREMEIVQEDRLRLRQEPGLDGRIIGHLAQGDQVIVIKEKGEWKQIVTKKAVGWVAASYLADAESP
TGSRQTGVVTADSLNVRVAPSLDAERIGRLLHGERVEIVETKRDWYKIVTRSGLGGWVAAEYIEMKNGQAVGNPEEAVAE
SASPAAVDLVTIQGNGPRQYVKKWTRTPVQVLAGKTIVLDAGHGGKDGGAESVNGVTEKTLTMETAERLKEKLETYGARV
VLTRVNDDYVPLSARVATARLYQADAFISLHYDSAEDEDASGITAYYYDRFADYGLAQSFQGPFSKLSALPFRGLAFGNY
YVLRENERPSVLLELGYLSNRSDAEVVATDSYQETVTTAIVNAVRHYFQ

Sequences:

>Translated_449_residues
MRPRRWFAILVCLCCLVTAAWPAEVKGENKKKERLAVVTVDRVNVRQGPGVPYRPLANVHRGETYRLIDIKDGWLKIEWK
KNKIGWIAASYAAPVREMEIVQEDRLRLRQEPGLDGRIIGHLAQGDQVIVIKEKGEWKQIVTKKAVGWVAASYLADAESP
TGSRQTGVVTADSLNVRVAPSLDAERIGRLLHGERVEIVETKRDWYKIVTRSGLGGWVAAEYIEMKNGQAVGNPEEAVAE
SASPAAVDLVTIQGNGPRQYVKKWTRTPVQVLAGKTIVLDAGHGGKDGGAESVNGVTEKTLTMETAERLKEKLETYGARV
VLTRVNDDYVPLSARVATARLYQADAFISLHYDSAEDEDASGITAYYYDRFADYGLAQSFQGPFSKLSALPFRGLAFGNY
YVLRENERPSVLLELGYLSNRSDAEVVATDSYQETVTTAIVNAVRHYFQ
>Mature_449_residues
MRPRRWFAILVCLCCLVTAAWPAEVKGENKKKERLAVVTVDRVNVRQGPGVPYRPLANVHRGETYRLIDIKDGWLKIEWK
KNKIGWIAASYAAPVREMEIVQEDRLRLRQEPGLDGRIIGHLAQGDQVIVIKEKGEWKQIVTKKAVGWVAASYLADAESP
TGSRQTGVVTADSLNVRVAPSLDAERIGRLLHGERVEIVETKRDWYKIVTRSGLGGWVAAEYIEMKNGQAVGNPEEAVAE
SASPAAVDLVTIQGNGPRQYVKKWTRTPVQVLAGKTIVLDAGHGGKDGGAESVNGVTEKTLTMETAERLKEKLETYGARV
VLTRVNDDYVPLSARVATARLYQADAFISLHYDSAEDEDASGITAYYYDRFADYGLAQSFQGPFSKLSALPFRGLAFGNY
YVLRENERPSVLLELGYLSNRSDAEVVATDSYQETVTTAIVNAVRHYFQ

Specific function: Probably involved in cell-wall metabolism [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=218, Percent_Identity=25.2293577981651, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788776, Length=224, Percent_Identity=25, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR017293
- InterPro:   IPR003646
- InterPro:   IPR013247 [H]

Pfam domain/function: PF01520 Amidase_3; PF08239 SH3_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 49817; Mature: 49817

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRRWFAILVCLCCLVTAAWPAEVKGENKKKERLAVVTVDRVNVRQGPGVPYRPLANVH
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCC
RGETYRLIDIKDGWLKIEWKKNKIGWIAASYAAPVREMEIVQEDRLRLRQEPGLDGRIIG
CCCEEEEEEECCCEEEEEEECCCEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEE
HLAQGDQVIVIKEKGEWKQIVTKKAVGWVAASYLADAESPTGSRQTGVVTADSLNVRVAP
EECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEECC
SLDAERIGRLLHGERVEIVETKRDWYKIVTRSGLGGWVAAEYIEMKNGQAVGNPEEAVAE
CCCHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHC
SASPAAVDLVTIQGNGPRQYVKKWTRTPVQVLAGKTIVLDAGHGGKDGGAESVNGVTEKT
CCCCCEEEEEEEECCCHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHH
LTMETAERLKEKLETYGARVVLTRVNDDYVPLSARVATARLYQADAFISLHYDSAEDEDA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHEEECCEEEEEEECCCCCCCC
SGITAYYYDRFADYGLAQSFQGPFSKLSALPFRGLAFGNYYVLRENERPSVLLELGYLSN
CCEEEEHHHHHHHCCHHHHCCCCHHHHHCCCCCCEEECCEEEEECCCCCEEEEEECCCCC
RSDAEVVATDSYQETVTTAIVNAVRHYFQ
CCCCEEEEECCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPRRWFAILVCLCCLVTAAWPAEVKGENKKKERLAVVTVDRVNVRQGPGVPYRPLANVH
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCC
RGETYRLIDIKDGWLKIEWKKNKIGWIAASYAAPVREMEIVQEDRLRLRQEPGLDGRIIG
CCCEEEEEEECCCEEEEEEECCCEEEEEEHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEE
HLAQGDQVIVIKEKGEWKQIVTKKAVGWVAASYLADAESPTGSRQTGVVTADSLNVRVAP
EECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEECC
SLDAERIGRLLHGERVEIVETKRDWYKIVTRSGLGGWVAAEYIEMKNGQAVGNPEEAVAE
CCCHHHHHHHHCCCEEEEEECHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHHC
SASPAAVDLVTIQGNGPRQYVKKWTRTPVQVLAGKTIVLDAGHGGKDGGAESVNGVTEKT
CCCCCEEEEEEEECCCHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHH
LTMETAERLKEKLETYGARVVLTRVNDDYVPLSARVATARLYQADAFISLHYDSAEDEDA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHEEECCEEEEEEECCCCCCCC
SGITAYYYDRFADYGLAQSFQGPFSKLSALPFRGLAFGNYYVLRENERPSVLLELGYLSN
CCEEEEHHHHHHHCCHHHHCCCCHHHHHCCCCCCEEECCEEEEECCCCCEEEEEECCCCC
RSDAEVVATDSYQETVTTAIVNAVRHYFQ
CCCCEEEEECCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]