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The map label for this gene is braE [H]

Identifier: 138896619

GI number: 138896619

Start: 3078279

End: 3079349

Strand: Reverse

Name: braE [H]

Synonym: GTNG_2982

Alternate gene names: 138896619

Gene position: 3079349-3078279 (Counterclockwise)

Preceding gene: 138896620

Following gene: 138896618

Centisome position: 86.73

GC content: 51.82

Gene sequence:

>1071_bases
ATGGCGGTGGAGCGGGATGAACGGATCAAAGGTGGACGAAGGCGGCTAGTGCTGCTCGTTTTGGTGGCCGCCTGGCTTGG
TTTCCCTTGGCTTGTGGCAGGAAACAATTATTTGTTGAGCACGCTCATTATCATTGGGGTCTATGCGCTAGTCAGTACAG
GGATGACGCTGTTGATGGGATACGCTGGGCAAATTTCGCTCGGCCAAGCCGCTTTTTATGGTGTCGGCGCTTACACGTCT
GCCTATTTGACCGCTCATGCAGGATGGCCGCCTTGGCTAGCGATGGCAGTTGGGGCGGTATTGGCCGCTTTGGTGGCGTT
TGTCGTGGGTATCCCGGTGTTTCGGTTGCGTGAGCATTATTTAGCCTTGGCGACGTTAGGATTTGGTGTCATTATGTTTA
CGCTGTTTAAGGAGTGGGAAGCGATCACCGGAGGGTTAAACGGCTTTTTTGGTATTCCGCCGATTAGCGTATTGGGGATG
TCCTTGCAGACCGATATTCAGTTTTATTACCTTGTTTGGCTGTTTGTCCTTGCCGGTTTATGGTTTGCCCATAACATCGT
CCGCTCTCGGGTCGGAAGGGCGTTGCGGGCGATTCACGGAAGCGAAGTGGCGGCCAGTTCACTTGGGGTGAACATCACCA
AATACAAGCTGCAAATCTTTATGCTTAGCGCCATGTATGCTTCGGTTGCCGGCAGTTTGTATGCCCATTATGTGACGTTT
ATCAACCCGGACTTATTTGGTATCGTTCCTTCGATTTATTTTCTCATTATGGTCGTCATTGGCGGAACGGCGGGCGTATG
GGGTGGCCTTGTTGGCGCTGCTGTTTATGTTTGCTTAGGCGAATGGCTAAAAGCGGTCGTTCCAATATTGATGCCGGATG
CCGGCGGGGAGTTTGAAATCGTCTTTTTTGGCTTGCTGCTTATTATCATGCTTATTTACATGCCTAGCGGATTGACAGGC
GCCGTGCAAAAGGGAATGAAACGCTTTAGGACAACGGCTGGAAGTCAGACAGGAACAACAGCAACGACCGAACGCCATGA
GGCGCGTTCGATAGGGGGAGGGCACGGATGA

Upstream 100 bases:

>100_bases
ATATAAAGACGCGGTTAGTTTCGGGTTGCTGTTGCTTGTACTCTTTGTCATGCCGAATGGATTGTTTGCGAAAATGACAG
GGAAACGGGTATAAGGAGGG

Downstream 100 bases:

>100_bases
GCGCCATGCTGTTGGAAGTCGAACAATTGTCCAAATCATTTGGCGGGGTTCAGGCGGTGCAACGTGTGTCATTTGGGGTG
AAAAAAGGAGACATCGTCGC

Product: branched chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAVERDERIKGGRRRLVLLVLVAAWLGFPWLVAGNNYLLSTLIIIGVYALVSTGMTLLMGYAGQISLGQAAFYGVGAYTS
AYLTAHAGWPPWLAMAVGAVLAALVAFVVGIPVFRLREHYLALATLGFGVIMFTLFKEWEAITGGLNGFFGIPPISVLGM
SLQTDIQFYYLVWLFVLAGLWFAHNIVRSRVGRALRAIHGSEVAASSLGVNITKYKLQIFMLSAMYASVAGSLYAHYVTF
INPDLFGIVPSIYFLIMVVIGGTAGVWGGLVGAAVYVCLGEWLKAVVPILMPDAGGEFEIVFFGLLLIIMLIYMPSGLTG
AVQKGMKRFRTTAGSQTGTTATTERHEARSIGGGHG

Sequences:

>Translated_356_residues
MAVERDERIKGGRRRLVLLVLVAAWLGFPWLVAGNNYLLSTLIIIGVYALVSTGMTLLMGYAGQISLGQAAFYGVGAYTS
AYLTAHAGWPPWLAMAVGAVLAALVAFVVGIPVFRLREHYLALATLGFGVIMFTLFKEWEAITGGLNGFFGIPPISVLGM
SLQTDIQFYYLVWLFVLAGLWFAHNIVRSRVGRALRAIHGSEVAASSLGVNITKYKLQIFMLSAMYASVAGSLYAHYVTF
INPDLFGIVPSIYFLIMVVIGGTAGVWGGLVGAAVYVCLGEWLKAVVPILMPDAGGEFEIVFFGLLLIIMLIYMPSGLTG
AVQKGMKRFRTTAGSQTGTTATTERHEARSIGGGHG
>Mature_355_residues
AVERDERIKGGRRRLVLLVLVAAWLGFPWLVAGNNYLLSTLIIIGVYALVSTGMTLLMGYAGQISLGQAAFYGVGAYTSA
YLTAHAGWPPWLAMAVGAVLAALVAFVVGIPVFRLREHYLALATLGFGVIMFTLFKEWEAITGGLNGFFGIPPISVLGMS
LQTDIQFYYLVWLFVLAGLWFAHNIVRSRVGRALRAIHGSEVAASSLGVNITKYKLQIFMLSAMYASVAGSLYAHYVTFI
NPDLFGIVPSIYFLIMVVIGGTAGVWGGLVGAAVYVCLGEWLKAVVPILMPDAGGEFEIVFFGLLLIIMLIYMPSGLTGA
VQKGMKRFRTTAGSQTGTTATTERHEARSIGGGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=322, Percent_Identity=31.3664596273292, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38262; Mature: 38131

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVERDERIKGGRRRLVLLVLVAAWLGFPWLVAGNNYLLSTLIIIGVYALVSTGMTLLMG
CCCCCCHHHCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
YAGQISLGQAAFYGVGAYTSAYLTAHAGWPPWLAMAVGAVLAALVAFVVGIPVFRLREHY
CCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LALATLGFGVIMFTLFKEWEAITGGLNGFFGIPPISVLGMSLQTDIQFYYLVWLFVLAGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
WFAHNIVRSRVGRALRAIHGSEVAASSLGVNITKYKLQIFMLSAMYASVAGSLYAHYVTF
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INPDLFGIVPSIYFLIMVVIGGTAGVWGGLVGAAVYVCLGEWLKAVVPILMPDAGGEFEI
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VFFGLLLIIMLIYMPSGLTGAVQKGMKRFRTTAGSQTGTTATTERHEARSIGGGHG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVERDERIKGGRRRLVLLVLVAAWLGFPWLVAGNNYLLSTLIIIGVYALVSTGMTLLMG
CCCCCHHHCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
YAGQISLGQAAFYGVGAYTSAYLTAHAGWPPWLAMAVGAVLAALVAFVVGIPVFRLREHY
CCCCCCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LALATLGFGVIMFTLFKEWEAITGGLNGFFGIPPISVLGMSLQTDIQFYYLVWLFVLAGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
WFAHNIVRSRVGRALRAIHGSEVAASSLGVNITKYKLQIFMLSAMYASVAGSLYAHYVTF
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INPDLFGIVPSIYFLIMVVIGGTAGVWGGLVGAAVYVCLGEWLKAVVPILMPDAGGEFEI
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
VFFGLLLIIMLIYMPSGLTGAVQKGMKRFRTTAGSQTGTTATTERHEARSIGGGHG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]