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The map label for this gene is gcvH [H]

Identifier: 138896593

GI number: 138896593

Start: 3053655

End: 3054038

Strand: Reverse

Name: gcvH [H]

Synonym: GTNG_2956

Alternate gene names: 138896593

Gene position: 3054038-3053655 (Counterclockwise)

Preceding gene: 138896594

Following gene: 138896592

Centisome position: 86.02

GC content: 48.44

Gene sequence:

>384_bases
ATGAACACGCCAAAAGAACTTCGCTATTCAAAAGAGCATGAGTGGGTGCGCGTCGAAGGAGACAAAGTGCGCATCGGCAT
TACCGATTTTGCCCAATCAGAGCTTGGCGACATCGTGTTTGTTGAGCTGCCGGAAGTTGGAACGGAAATTACGGCGAATG
AGCCGTTTGGTAGCGTCGAATCGGTGAAAACCGTCTCGGAACTGTATGCGCCGATCAGCGGCACGATCGTCGAAGTGAAC
GAATCGTTAAATGACAATCCGGAATATGTGAACGAATCGCCGTATGAAAAAGCGTGGATGATTGTTATTGAACCGAAAGA
TTTAAGCGAAGTCGACAACTTGTTGACGGCTGAACAATACGCGGCGATGGTGAACGAAGGATAA

Upstream 100 bases:

>100_bases
AAGATAAAAAATTTTAATAAAATGCGACGAACTCGGTGAGTTTTTGTCCAAAATCGGTTATGATGAAAAAGGAGATAATT
TGTCGCAATGGAGGGATTCC

Downstream 100 bases:

>100_bases
ACGTTCCTGCAATCTTCGCCCCCGATCCGGACACCCTATACATACCGGAACTTGATTGAAAAGGGGGGCTCCCGATGGCG
CTGCAATCAACGCGCATCGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MNTPKELRYSKEHEWVRVEGDKVRIGITDFAQSELGDIVFVELPEVGTEITANEPFGSVESVKTVSELYAPISGTIVEVN
ESLNDNPEYVNESPYEKAWMIVIEPKDLSEVDNLLTAEQYAAMVNEG

Sequences:

>Translated_127_residues
MNTPKELRYSKEHEWVRVEGDKVRIGITDFAQSELGDIVFVELPEVGTEITANEPFGSVESVKTVSELYAPISGTIVEVN
ESLNDNPEYVNESPYEKAWMIVIEPKDLSEVDNLLTAEQYAAMVNEG
>Mature_127_residues
MNTPKELRYSKEHEWVRVEGDKVRIGITDFAQSELGDIVFVELPEVGTEITANEPFGSVESVKTVSELYAPISGTIVEVN
ESLNDNPEYVNESPYEKAWMIVIEPKDLSEVDNLLTAEQYAAMVNEG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=46.0176991150442, Blast_Score=117, Evalue=3e-27,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=45.1327433628319, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=50, Blast_Score=131, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=49.5575221238938, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=47.4576271186441, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=45.4545454545455, Blast_Score=114, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17865652, Length=122, Percent_Identity=44.2622950819672, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14224; Mature: 14224

Theoretical pI: Translated: 3.87; Mature: 3.87

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPKELRYSKEHEWVRVEGDKVRIGITDFAQSELGDIVFVELPEVGTEITANEPFGSVE
CCCCHHCCCCCCCCEEEEECCEEEEEEHHHHHHCCCCEEEEECCCCCCEEECCCCCCCHH
SVKTVSELYAPISGTIVEVNESLNDNPEYVNESPYEKAWMIVIEPKDLSEVDNLLTAEQY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
AAMVNEG
HHHHCCC
>Mature Secondary Structure
MNTPKELRYSKEHEWVRVEGDKVRIGITDFAQSELGDIVFVELPEVGTEITANEPFGSVE
CCCCHHCCCCCCCCEEEEECCEEEEEEHHHHHHCCCCEEEEECCCCCCEEECCCCCCCHH
SVKTVSELYAPISGTIVEVNESLNDNPEYVNESPYEKAWMIVIEPKDLSEVDNLLTAEQY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
AAMVNEG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA