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The map label for this gene is braC [H]

Identifier: 138896567

GI number: 138896567

Start: 3032371

End: 3033576

Strand: Reverse

Name: braC [H]

Synonym: GTNG_2930

Alternate gene names: 138896567

Gene position: 3033576-3032371 (Counterclockwise)

Preceding gene: 138896568

Following gene: 138896566

Centisome position: 85.45

GC content: 50.66

Gene sequence:

>1206_bases
ATGGGGGGAAAACGAGTGAAAAAGAAAAAAGTAGCAGGTGTCTTTTTCTCGCTCATGCTGACAGCGGGTCTCATGGCCGG
CTGCGGCGGCAACGAACAAAGCAGCGGCTCGACCGGCGGCAGCGGCGGTGGAGGTGGGGATGTCATTAAAATCGGCGCCA
ACCTCGAGCTGTCCGGCGGGGTCGCTTCGTACGGACAGTCGATCGCTGAAGGGGTCGAGCTGGCGTTTGATGAAATCAAC
AAAGAAGGAATCAATGGCAAAAAGTTAGAACTTGTCAAAGTCGATAACAAATCGGAAGCAGCCGAAGCGACGAACGGCGC
GATCAAGTTGATCAGCCAAGATAAAGTCGTTGCGATTGTCGGCTCGGCAACGAGTACGAACACACTCGCTCAAGTGCAAA
TCGCCAATGACAATAAAGTGCCGGTGATTACGCCGTCCGGTACGAACCCGACGATTACTACCAAAGACGGAAAAGTGAAT
GATTTCATTTTCCGGACATGCTTTATCGACCCGTTCCAAGGAACGGTGGCGGCAAAATTCGCATTGGATGACTTGAAAGT
GAAAAGTGCGGCGATTTTGATCGACAGCGCGAGCGATTACTCGAAAGGGCTGGCTGCGTCATTTAAAGAAACGTTTACAG
CCGGCGGCGGCAAAATTGTTGCCGAAGAAGCGTATGTGGCGAAAGATACGGACTTCCGCGCCACGCTGACGAACATTAAA
GCGGCCAATCCGGAATTTTTGTTCTTGCCGGGCTATTATGAAGAGGTTGGCCTCATCGTGAAACAAGCGCGTGAGCTTGG
TTTGAACGTGCCGATCATGGGCGGCGACGGCTGGGATTCGCCGAAGTTGATTGAGATCGCCGGCAAAGATGCGTTGAACA
ATACGTATATTACCAACCATTATTCATCCGGTGACCCGGATCCGAAAATTCAAGAGTTCGTCAAAGCGTTCGAAGCGAAA
TACAATAAAGCGCCGGATGCCTTTAACGCGCTTGGCTACGATACGGCTTACTTCCTGGCGGATGCCATCAAACGGGCTGG
CAGCGCCGATCCGGTGAAAATTAAAGACGCGCTGGCACAAACGAAAGATTTGCAGTTGGTGACTGGTATATTCACGATCA
ATGAAAATCACGATCCGATCAAATCGGCAGTCATTTTGGAATACAAAGATGGGCAGCAACAATTCAAAACAAAAGTGAAC
CCGTAA

Upstream 100 bases:

>100_bases
TTCTCATGCAACCGCTCCTTCACGACTACAAGTGCGCATGCAGAGGGTTTTTCGCCGATACCGTTAAACTGCGCACTCCG
ATGACAGAACATTATGAACA

Downstream 100 bases:

>100_bases
TGAAAGGCTGGATGGAGTGCATGGGCGGGGGAGGGCGTCCCCCTGGGGGACGTCTTCCTGTCCAGCATTTCTTTTGGCAA
ACGACGAGTTTTCGTTAAGG

Product: branched chain amino acid ABC transporter amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MGGKRVKKKKVAGVFFSLMLTAGLMAGCGGNEQSSGSTGGSGGGGGDVIKIGANLELSGGVASYGQSIAEGVELAFDEIN
KEGINGKKLELVKVDNKSEAAEATNGAIKLISQDKVVAIVGSATSTNTLAQVQIANDNKVPVITPSGTNPTITTKDGKVN
DFIFRTCFIDPFQGTVAAKFALDDLKVKSAAILIDSASDYSKGLAASFKETFTAGGGKIVAEEAYVAKDTDFRATLTNIK
AANPEFLFLPGYYEEVGLIVKQARELGLNVPIMGGDGWDSPKLIEIAGKDALNNTYITNHYSSGDPDPKIQEFVKAFEAK
YNKAPDAFNALGYDTAYFLADAIKRAGSADPVKIKDALAQTKDLQLVTGIFTINENHDPIKSAVILEYKDGQQQFKTKVN
P

Sequences:

>Translated_401_residues
MGGKRVKKKKVAGVFFSLMLTAGLMAGCGGNEQSSGSTGGSGGGGGDVIKIGANLELSGGVASYGQSIAEGVELAFDEIN
KEGINGKKLELVKVDNKSEAAEATNGAIKLISQDKVVAIVGSATSTNTLAQVQIANDNKVPVITPSGTNPTITTKDGKVN
DFIFRTCFIDPFQGTVAAKFALDDLKVKSAAILIDSASDYSKGLAASFKETFTAGGGKIVAEEAYVAKDTDFRATLTNIK
AANPEFLFLPGYYEEVGLIVKQARELGLNVPIMGGDGWDSPKLIEIAGKDALNNTYITNHYSSGDPDPKIQEFVKAFEAK
YNKAPDAFNALGYDTAYFLADAIKRAGSADPVKIKDALAQTKDLQLVTGIFTINENHDPIKSAVILEYKDGQQQFKTKVN
P
>Mature_400_residues
GGKRVKKKKVAGVFFSLMLTAGLMAGCGGNEQSSGSTGGSGGGGGDVIKIGANLELSGGVASYGQSIAEGVELAFDEINK
EGINGKKLELVKVDNKSEAAEATNGAIKLISQDKVVAIVGSATSTNTLAQVQIANDNKVPVITPSGTNPTITTKDGKVND
FIFRTCFIDPFQGTVAAKFALDDLKVKSAAILIDSASDYSKGLAASFKETFTAGGGKIVAEEAYVAKDTDFRATLTNIKA
ANPEFLFLPGYYEEVGLIVKQARELGLNVPIMGGDGWDSPKLIEIAGKDALNNTYITNHYSSGDPDPKIQEFVKAFEAKY
NKAPDAFNALGYDTAYFLADAIKRAGSADPVKIKDALAQTKDLQLVTGIFTINENHDPIKSAVILEYKDGQQQFKTKVNP

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=322, Percent_Identity=31.9875776397516, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI48994941, Length=331, Percent_Identity=30.5135951661631, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42315; Mature: 42184

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGKRVKKKKVAGVFFSLMLTAGLMAGCGGNEQSSGSTGGSGGGGGDVIKIGANLELSGG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCC
VASYGQSIAEGVELAFDEINKEGINGKKLELVKVDNKSEAAEATNGAIKLISQDKVVAIV
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCEEEEEECCCEEEEE
GSATSTNTLAQVQIANDNKVPVITPSGTNPTITTKDGKVNDFIFRTCFIDPFQGTVAAKF
ECCCCCCEEEEEEECCCCCEEEEECCCCCCEEEECCCCCCCEEEEEEEECCCCCCEEEEE
ALDDLKVKSAAILIDSASDYSKGLAASFKETFTAGGGKIVAEEAYVAKDTDFRATLTNIK
EHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCCEEEEECCC
AANPEFLFLPGYYEEVGLIVKQARELGLNVPIMGGDGWDSPKLIEIAGKDALNNTYITNH
CCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCCCEEEEC
YSSGDPDPKIQEFVKAFEAKYNKAPDAFNALGYDTAYFLADAIKRAGSADPVKIKDALAQ
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEHHHHHHC
TKDLQLVTGIFTINENHDPIKSAVILEYKDGQQQFKTKVNP
CCCCEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHCCCCC
>Mature Secondary Structure 
GGKRVKKKKVAGVFFSLMLTAGLMAGCGGNEQSSGSTGGSGGGGGDVIKIGANLELSGG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCC
VASYGQSIAEGVELAFDEINKEGINGKKLELVKVDNKSEAAEATNGAIKLISQDKVVAIV
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHCCCCEEEEEECCCEEEEE
GSATSTNTLAQVQIANDNKVPVITPSGTNPTITTKDGKVNDFIFRTCFIDPFQGTVAAKF
ECCCCCCEEEEEEECCCCCEEEEECCCCCCEEEECCCCCCCEEEEEEEECCCCCCEEEEE
ALDDLKVKSAAILIDSASDYSKGLAASFKETFTAGGGKIVAEEAYVAKDTDFRATLTNIK
EHHHHEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCCEEEEECCC
AANPEFLFLPGYYEEVGLIVKQARELGLNVPIMGGDGWDSPKLIEIAGKDALNNTYITNH
CCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCCCEEEEC
YSSGDPDPKIQEFVKAFEAKYNKAPDAFNALGYDTAYFLADAIKRAGSADPVKIKDALAQ
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEHHHHHHC
TKDLQLVTGIFTINENHDPIKSAVILEYKDGQQQFKTKVNP
CCCCEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]