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The map label for this gene is mrpA [H]

Identifier: 138896530

GI number: 138896530

Start: 2999247

End: 3001643

Strand: Reverse

Name: mrpA [H]

Synonym: GTNG_2893

Alternate gene names: 138896530

Gene position: 3001643-2999247 (Counterclockwise)

Preceding gene: 138896532

Following gene: 138896529

Centisome position: 84.55

GC content: 49.73

Gene sequence:

>2397_bases
ATGTTAAACTTGGCAATTGTTTCACCACTGATTTTCGCTTTATTCATTCCGTTTTTGTATAAATATGTGCGTTCGATTCA
TACTGGATGGTTTGTGCTCCCTCTTCCGGTCTTATTGTTTGTGTATTTTATTCAACATCTCCCTCTTGAACCCTACGGAC
AAACGATCCCTTGGATTCCGTCTCTCGGAATGGATTTTCAAGTCTATATGGATGGGTTAGGCTTGCTCTTTTCGCTGTTA
ATCACAGGGATCGGCTCTCTTGTCGTGTTGTATTCGATTTATTATTTGCCAAAGGAAAAGGAAAAACTTGGTCACTTTTA
CGTGTATTTGCTTTTGTTTATGGGAGCGATGCTTGGCGTCGTTCTTTCAGACAACGCCATGGCGCTCTATCTGTTTTGGG
AATTGACATCGTTTTCATCCTTTTTGCTGATTGGCTACTGGAATGAGCGTGAACGTTCCCGATACGGGGCCCAAAAATCC
ATGCTCATCACCGTGCTTGGCGGGCTGTCTCTGCTTGGTGGATTTTTATTGCTTTCGGTCATGAGCGGAGGGTCGTACAG
CATTCGTGACTGGATCGATATGGCGGATGCGTGGCCGCAACATCCGTTATTCTTGCCAGCGATGCTCTTGATTTTGCTTG
GCGCGTTCACCAAATCCGCGCAGTTTCCATTTTACATTTGGCTTCCGGACGCGATGGAAGCGCCCACCCCAGTCAGTGCG
TATTTACATTCGGCGACGATGGTGAAAGCCGGCATTTATTTAGTGGCTCGTTTCACCCCGATTTTTGCTGTTTCTTCGCT
ATGGGTTTGGCTTGTGATGGGCATTGGAATGGCGACGCTTTGCTGGGCGTCATTTCATGCCGCCCGCCAGACAGACTTGA
AAGGGTTGCTCGCCTATTCGACGGTGAGCCAGCTCGGGTTGATTATGTCGTTGCTCGGCGCTGGGGCGCTGGCCTTTCAT
GGTGATCCGAGTGATCGCCGCTTGTATATGGCAGCCGTGGTTGCAGCGGTGTTTCACTTGATCAACCATGCGACGTTCAA
AGGAAGTTTGTTTATGACTGCCGGCATTGTCGATCATGAGACCGGGACGCGAGATATCCGGCGTCTTGGCGGCTTGCTTA
GCTTCATGCCCATCACATTTACAATTGCGGTCATTGGTTCGTTGTCGATGGCGGGTGTTCCTCCGTTTAACGGATTTTTA
AGCAAAGAAATGTTTTTTGCTGCGATGGTGCAAGTGGCGAACGCCCATTGGTTTTCGCTTGATGTCTGGGGGATCTTATT
TCCGGTGCTCGCTTGGGTAGGAAGCGTGTTTACCTTTTTATATAGTCTTCTTTTTGTTGGGAAAACGTTTCTTGGCGCCC
CGAAGCCTTCCGAATGGCCGAAACAGCCGCATGAAGCTCCGGTGGGGATGCTTGTGGCGCCGGTCGTTTTGGCTGTCCTT
GTCATTGGCTTCGGACTGTTTCCGAATGTGTTGTCCAATGCGGTGATTGCTCCGGCGGCCGATGCCATTGTCAACGGTGT
GTTCCGCCAGGAAGGGCTTTCTGTGCACATTAGCCATTGGCACGGATGGACGATCGAAGTATGGATGACCATCGGAGTGA
TTGTCCTTGGCGTGTTGCTGTATCGGACGTTTGATTCATGGAAGCGCGTGTACGGTGCGTTTTCGCAGCGCCTTTCATTG
AATCACTTATATGACACGGTTGTGCGGCAGATGGAAGAGCGGTCTCATTCCTTGACAAGCCGTTACATGACCGGGTATGT
TCGGACGTATTTCTTGTATATTTTGTCCGCGATGCTCGCGTTGCTTATTGTGACGATCGTTCTGAAAGTGGATGGGGAGT
TTTCGTTAAGCCATTTAGCGGACGTGGGCGTCCATGAACTGATGTTGGTGTTGGCAGCTTTGGTCGGAACGGTGACGACT
TTAGTGGCCAAATCACGCCTTGTCGCCATTATCGCTTTAGGTTCGGTCGGCTATACGGTCTCGCTTTTCTTTGTGCTCTT
CCGGGCGCCTGACTTGGCATTAACCCAACTTGTCATTGAAACGATTTCCGTCGCTCTGTTTTTGCTTTGTTTCTATCACT
TGCCGAAGCTTAGCCGCCATGAGAAAGGCGTCCGTTTCCGTCTTGGGAACGCCCTCATTTCGCTTGGCGTCGGCGCATTG
GTCAGCGTGATCGCCCTGTTGGCGTACAGCGAGAAAAACTTTGCGTCGATTGCCCAATACCATATCGAAAACGCGTATGA
AAAAGCGGCTGGAAAAAATATTGTTAACGTGATCTTAGTCGACTTCCGCGGATTTGATACGCTATTTGAAATTTGTGTGC
TGGCGATCGCGGCGCTCGGCATTTACGCCTTAGTGAAGCTGAAAGCTCCGAAAGGGGAGAACGGCAAGTATGAATGA

Upstream 100 bases:

>100_bases
AGGTGGGAGTTTATGATACAATACGAAGTAGGATAAGCGGGGCGATGCGAGGGGGAAAAGGAAACATGACAGTTGGTTAG
TTAGGGAAGGAGGATGGTTT

Downstream 100 bases:

>100_bases
ACTCATTTTGCGGACGGTGACGGCCGTTGTCACGTTTATCATCATTTTGTTCGGGATTCAGCTTTTTTTCGCCGGCCACT
ATTATCCGGGCGGCGGATTC

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 798; Mature: 798

Protein sequence:

>798_residues
MLNLAIVSPLIFALFIPFLYKYVRSIHTGWFVLPLPVLLFVYFIQHLPLEPYGQTIPWIPSLGMDFQVYMDGLGLLFSLL
ITGIGSLVVLYSIYYLPKEKEKLGHFYVYLLLFMGAMLGVVLSDNAMALYLFWELTSFSSFLLIGYWNERERSRYGAQKS
MLITVLGGLSLLGGFLLLSVMSGGSYSIRDWIDMADAWPQHPLFLPAMLLILLGAFTKSAQFPFYIWLPDAMEAPTPVSA
YLHSATMVKAGIYLVARFTPIFAVSSLWVWLVMGIGMATLCWASFHAARQTDLKGLLAYSTVSQLGLIMSLLGAGALAFH
GDPSDRRLYMAAVVAAVFHLINHATFKGSLFMTAGIVDHETGTRDIRRLGGLLSFMPITFTIAVIGSLSMAGVPPFNGFL
SKEMFFAAMVQVANAHWFSLDVWGILFPVLAWVGSVFTFLYSLLFVGKTFLGAPKPSEWPKQPHEAPVGMLVAPVVLAVL
VIGFGLFPNVLSNAVIAPAADAIVNGVFRQEGLSVHISHWHGWTIEVWMTIGVIVLGVLLYRTFDSWKRVYGAFSQRLSL
NHLYDTVVRQMEERSHSLTSRYMTGYVRTYFLYILSAMLALLIVTIVLKVDGEFSLSHLADVGVHELMLVLAALVGTVTT
LVAKSRLVAIIALGSVGYTVSLFFVLFRAPDLALTQLVIETISVALFLLCFYHLPKLSRHEKGVRFRLGNALISLGVGAL
VSVIALLAYSEKNFASIAQYHIENAYEKAAGKNIVNVILVDFRGFDTLFEICVLAIAALGIYALVKLKAPKGENGKYE

Sequences:

>Translated_798_residues
MLNLAIVSPLIFALFIPFLYKYVRSIHTGWFVLPLPVLLFVYFIQHLPLEPYGQTIPWIPSLGMDFQVYMDGLGLLFSLL
ITGIGSLVVLYSIYYLPKEKEKLGHFYVYLLLFMGAMLGVVLSDNAMALYLFWELTSFSSFLLIGYWNERERSRYGAQKS
MLITVLGGLSLLGGFLLLSVMSGGSYSIRDWIDMADAWPQHPLFLPAMLLILLGAFTKSAQFPFYIWLPDAMEAPTPVSA
YLHSATMVKAGIYLVARFTPIFAVSSLWVWLVMGIGMATLCWASFHAARQTDLKGLLAYSTVSQLGLIMSLLGAGALAFH
GDPSDRRLYMAAVVAAVFHLINHATFKGSLFMTAGIVDHETGTRDIRRLGGLLSFMPITFTIAVIGSLSMAGVPPFNGFL
SKEMFFAAMVQVANAHWFSLDVWGILFPVLAWVGSVFTFLYSLLFVGKTFLGAPKPSEWPKQPHEAPVGMLVAPVVLAVL
VIGFGLFPNVLSNAVIAPAADAIVNGVFRQEGLSVHISHWHGWTIEVWMTIGVIVLGVLLYRTFDSWKRVYGAFSQRLSL
NHLYDTVVRQMEERSHSLTSRYMTGYVRTYFLYILSAMLALLIVTIVLKVDGEFSLSHLADVGVHELMLVLAALVGTVTT
LVAKSRLVAIIALGSVGYTVSLFFVLFRAPDLALTQLVIETISVALFLLCFYHLPKLSRHEKGVRFRLGNALISLGVGAL
VSVIALLAYSEKNFASIAQYHIENAYEKAAGKNIVNVILVDFRGFDTLFEICVLAIAALGIYALVKLKAPKGENGKYE
>Mature_798_residues
MLNLAIVSPLIFALFIPFLYKYVRSIHTGWFVLPLPVLLFVYFIQHLPLEPYGQTIPWIPSLGMDFQVYMDGLGLLFSLL
ITGIGSLVVLYSIYYLPKEKEKLGHFYVYLLLFMGAMLGVVLSDNAMALYLFWELTSFSSFLLIGYWNERERSRYGAQKS
MLITVLGGLSLLGGFLLLSVMSGGSYSIRDWIDMADAWPQHPLFLPAMLLILLGAFTKSAQFPFYIWLPDAMEAPTPVSA
YLHSATMVKAGIYLVARFTPIFAVSSLWVWLVMGIGMATLCWASFHAARQTDLKGLLAYSTVSQLGLIMSLLGAGALAFH
GDPSDRRLYMAAVVAAVFHLINHATFKGSLFMTAGIVDHETGTRDIRRLGGLLSFMPITFTIAVIGSLSMAGVPPFNGFL
SKEMFFAAMVQVANAHWFSLDVWGILFPVLAWVGSVFTFLYSLLFVGKTFLGAPKPSEWPKQPHEAPVGMLVAPVVLAVL
VIGFGLFPNVLSNAVIAPAADAIVNGVFRQEGLSVHISHWHGWTIEVWMTIGVIVLGVLLYRTFDSWKRVYGAFSQRLSL
NHLYDTVVRQMEERSHSLTSRYMTGYVRTYFLYILSAMLALLIVTIVLKVDGEFSLSHLADVGVHELMLVLAALVGTVTT
LVAKSRLVAIIALGSVGYTVSLFFVLFRAPDLALTQLVIETISVALFLLCFYHLPKLSRHEKGVRFRLGNALISLGVGAL
VSVIALLAYSEKNFASIAQYHIENAYEKAAGKNIVNVILVDFRGFDTLFEICVLAIAALGIYALVKLKAPKGENGKYE

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=445, Percent_Identity=32.1348314606742, Blast_Score=179, Evalue=9e-45,
Organism=Escherichia coli, GI1788614, Length=491, Percent_Identity=32.7902240325866, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI1788829, Length=434, Percent_Identity=33.6405529953917, Blast_Score=175, Evalue=1e-44,
Organism=Escherichia coli, GI1788831, Length=362, Percent_Identity=33.9779005524862, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788827, Length=427, Percent_Identity=29.03981264637, Blast_Score=139, Evalue=8e-34,
Organism=Escherichia coli, GI1788613, Length=413, Percent_Identity=27.8450363196126, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI2367154, Length=286, Percent_Identity=30.4195804195804, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693160, Length=428, Percent_Identity=26.8691588785047, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 88178; Mature: 88178

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLAIVSPLIFALFIPFLYKYVRSIHTGWFVLPLPVLLFVYFIQHLPLEPYGQTIPWIP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SLGMDFQVYMDGLGLLFSLLITGIGSLVVLYSIYYLPKEKEKLGHFYVYLLLFMGAMLGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VLSDNAMALYLFWELTSFSSFLLIGYWNERERSRYGAQKSMLITVLGGLSLLGGFLLLSV
HHCCCCEEEEEEHHHHCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MSGGSYSIRDWIDMADAWPQHPLFLPAMLLILLGAFTKSAQFPFYIWLPDAMEAPTPVSA
HCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHH
YLHSATMVKAGIYLVARFTPIFAVSSLWVWLVMGIGMATLCWASFHAARQTDLKGLLAYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVSQLGLIMSLLGAGALAFHGDPSDRRLYMAAVVAAVFHLINHATFKGSLFMTAGIVDHE
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEECC
TGTRDIRRLGGLLSFMPITFTIAVIGSLSMAGVPPFNGFLSKEMFFAAMVQVANAHWFSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEH
DVWGILFPVLAWVGSVFTFLYSLLFVGKTFLGAPKPSEWPKQPHEAPVGMLVAPVVLAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VIGFGLFPNVLSNAVIAPAADAIVNGVFRQEGLSVHISHWHGWTIEVWMTIGVIVLGVLL
HHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
YRTFDSWKRVYGAFSQRLSLNHLYDTVVRQMEERSHSLTSRYMTGYVRTYFLYILSAMLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIVTIVLKVDGEFSLSHLADVGVHELMLVLAALVGTVTTLVAKSRLVAIIALGSVGYTV
HHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHH
SLFFVLFRAPDLALTQLVIETISVALFLLCFYHLPKLSRHEKGVRFRLGNALISLGVGAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
VSVIALLAYSEKNFASIAQYHIENAYEKAAGKNIVNVILVDFRGFDTLFEICVLAIAALG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHH
IYALVKLKAPKGENGKYE
HHHHHHEECCCCCCCCCC
>Mature Secondary Structure
MLNLAIVSPLIFALFIPFLYKYVRSIHTGWFVLPLPVLLFVYFIQHLPLEPYGQTIPWIP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SLGMDFQVYMDGLGLLFSLLITGIGSLVVLYSIYYLPKEKEKLGHFYVYLLLFMGAMLGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VLSDNAMALYLFWELTSFSSFLLIGYWNERERSRYGAQKSMLITVLGGLSLLGGFLLLSV
HHCCCCEEEEEEHHHHCCCCEEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MSGGSYSIRDWIDMADAWPQHPLFLPAMLLILLGAFTKSAQFPFYIWLPDAMEAPTPVSA
HCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHH
YLHSATMVKAGIYLVARFTPIFAVSSLWVWLVMGIGMATLCWASFHAARQTDLKGLLAYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVSQLGLIMSLLGAGALAFHGDPSDRRLYMAAVVAAVFHLINHATFKGSLFMTAGIVDHE
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEECC
TGTRDIRRLGGLLSFMPITFTIAVIGSLSMAGVPPFNGFLSKEMFFAAMVQVANAHWFSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEH
DVWGILFPVLAWVGSVFTFLYSLLFVGKTFLGAPKPSEWPKQPHEAPVGMLVAPVVLAVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VIGFGLFPNVLSNAVIAPAADAIVNGVFRQEGLSVHISHWHGWTIEVWMTIGVIVLGVLL
HHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
YRTFDSWKRVYGAFSQRLSLNHLYDTVVRQMEERSHSLTSRYMTGYVRTYFLYILSAMLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIVTIVLKVDGEFSLSHLADVGVHELMLVLAALVGTVTTLVAKSRLVAIIALGSVGYTV
HHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHH
SLFFVLFRAPDLALTQLVIETISVALFLLCFYHLPKLSRHEKGVRFRLGNALISLGVGAL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
VSVIALLAYSEKNFASIAQYHIENAYEKAAGKNIVNVILVDFRGFDTLFEICVLAIAALG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHH
IYALVKLKAPKGENGKYE
HHHHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]