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The map label for this gene is mrpD [H]

Identifier: 138896527

GI number: 138896527

Start: 2997031

End: 2998503

Strand: Reverse

Name: mrpD [H]

Synonym: GTNG_2890

Alternate gene names: 138896527

Gene position: 2998503-2997031 (Counterclockwise)

Preceding gene: 138896528

Following gene: 138896526

Centisome position: 84.46

GC content: 49.22

Gene sequence:

>1473_bases
ATGACTAATGCAGTAATTTTTCCGATTGCCATTCCATTGACGACGGCGGTTATCTTGATTTTTTGTGTGAAATCGTTGAA
GGCGCAAAAGGTGATTTCCCTCGTTTCAGCGGGGGCGCTCATCGCTGCGGGCGCTTGGCTTGTGCGCCTTGTCGATCATG
AAGGGGTGCAAAAGCTCGACGTCGGAAACTGGCCGGCGCCGTTTGGGATTACGCTCGTCTCCGACATGCTTTCGGCGCTG
TTAGTGTTGACGACGAGCGTGATCGCGCTTGCTGGTCTATTGTATTCCTTTTACACGATTGACTCGAAGCAGGCGTCCAT
GTACTACTATGTTTTTTTTCAGTTCCTCATTGTCGGAGTGAACGGGGCGTTTAGTACAGGGGATATTTTTAACTTATTCG
TGTTTTATGAAGTGATGCTCATGTCATCGTATGCACTGCTCGTTCACGGCGGCACCAAAATCCAGCTCCAGGAAACGATG
AAATACATGATCATCAATGTGTTTTCTTCCGCGTTGTTTGTCATCGCCGTCGGCTATTTGTACGCTGTGACCGGCACATT
GAATATGGCGCACTTAGCCGTACGGATTTCTGGAGCGGAAAACACAGCCGTGTTGACGGTCATTTGTTTGCTTTTTCTCA
TTGTATTTGGGTTAAAAGGGGCGTTGTTTCCGTTTTATGTTTGGATGCCAGGAGCCTATTCTGCTCCGCCATCGGCCGTG
CTTGCGTTGTTCGGCGGCTTGTTGACGAAAGTCGGTGTGTATTCGATTTTGCGTACTTTTTCGCTGATTTTCCGCCAAGA
TATCGGTTATACTCATACCATCTTAGCGTGGTTGGCGATGGCGACCATTGTGCTTGGCGTCATTGGTGCGGTGGCTTACC
GCGATATGCGGCAAATTGCCATTTATAACATTGTCGCGGCTATCGGTGTGATGGCGTTTGGCATTTCTTTAATGACAAAA
GAAAGTATGGAAGGCACGATCTTTTACTTGCTGCAAGACATGGTGATGAAAACTGCGCTCTTTTTTATTGTCGGTGCGAT
CGCGTATGTCGCAGGAACGAATCAACTCGGACGGTTTAGCGGGCTGCTTGGTTCGTATCCGTTGCTTGGCTGGGCCGTAT
TCCTTACCGCTCTTGCGCTTAGCGGCATTCCTCCGTTTAGCGGGTTTATCGGAAAAGCGCTGATTATTCGGGCGGCGTTT
GAAAAAGGACAGCTTCTGTTCGCACTGATTGTGTTATTGTCCAGTTTACTCGTGCTTTATTCGGTCATGAAAATTTTTAT
TCAATCTTGTTGGGGAGAAGCGCGCGGATATGAGCAAAAACGAGTCGCCCCGCTGTATGTGCCGATTGTCGCGCTTCTTG
CCTTGGCCGTTTTATACGGGATCGGGGCCGAATTTGTTCGTCCATACATCGCCCAAGCGGCGGCGACGTTGGCAGATCCG
TCGCTGTATATTGAGTCCGTTCTGAAGGAGTAG

Upstream 100 bases:

>100_bases
ATTAGCTTTGGTGTCACGGCTTTTTTGCTCGTTTTGGCGTATCGTTCTTATCAGGAGATTGGAACGGACAACATTGAACA
AATGAGGGGAAAGGAAGACC

Downstream 100 bases:

>100_bases
AGGCAAATGGCACAGCAATTGCTGCTCAACGTTCTATTGGCGTTCGTTTGGATGTTTCTTGCTGATTCATTTAGCGGGTC
ATCGTTTGTCATTGGATATC

Product: Na+/H+ antiporter

Products: NA

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTNAVIFPIAIPLTTAVILIFCVKSLKAQKVISLVSAGALIAAGAWLVRLVDHEGVQKLDVGNWPAPFGITLVSDMLSAL
LVLTTSVIALAGLLYSFYTIDSKQASMYYYVFFQFLIVGVNGAFSTGDIFNLFVFYEVMLMSSYALLVHGGTKIQLQETM
KYMIINVFSSALFVIAVGYLYAVTGTLNMAHLAVRISGAENTAVLTVICLLFLIVFGLKGALFPFYVWMPGAYSAPPSAV
LALFGGLLTKVGVYSILRTFSLIFRQDIGYTHTILAWLAMATIVLGVIGAVAYRDMRQIAIYNIVAAIGVMAFGISLMTK
ESMEGTIFYLLQDMVMKTALFFIVGAIAYVAGTNQLGRFSGLLGSYPLLGWAVFLTALALSGIPPFSGFIGKALIIRAAF
EKGQLLFALIVLLSSLLVLYSVMKIFIQSCWGEARGYEQKRVAPLYVPIVALLALAVLYGIGAEFVRPYIAQAAATLADP
SLYIESVLKE

Sequences:

>Translated_490_residues
MTNAVIFPIAIPLTTAVILIFCVKSLKAQKVISLVSAGALIAAGAWLVRLVDHEGVQKLDVGNWPAPFGITLVSDMLSAL
LVLTTSVIALAGLLYSFYTIDSKQASMYYYVFFQFLIVGVNGAFSTGDIFNLFVFYEVMLMSSYALLVHGGTKIQLQETM
KYMIINVFSSALFVIAVGYLYAVTGTLNMAHLAVRISGAENTAVLTVICLLFLIVFGLKGALFPFYVWMPGAYSAPPSAV
LALFGGLLTKVGVYSILRTFSLIFRQDIGYTHTILAWLAMATIVLGVIGAVAYRDMRQIAIYNIVAAIGVMAFGISLMTK
ESMEGTIFYLLQDMVMKTALFFIVGAIAYVAGTNQLGRFSGLLGSYPLLGWAVFLTALALSGIPPFSGFIGKALIIRAAF
EKGQLLFALIVLLSSLLVLYSVMKIFIQSCWGEARGYEQKRVAPLYVPIVALLALAVLYGIGAEFVRPYIAQAAATLADP
SLYIESVLKE
>Mature_489_residues
TNAVIFPIAIPLTTAVILIFCVKSLKAQKVISLVSAGALIAAGAWLVRLVDHEGVQKLDVGNWPAPFGITLVSDMLSALL
VLTTSVIALAGLLYSFYTIDSKQASMYYYVFFQFLIVGVNGAFSTGDIFNLFVFYEVMLMSSYALLVHGGTKIQLQETMK
YMIINVFSSALFVIAVGYLYAVTGTLNMAHLAVRISGAENTAVLTVICLLFLIVFGLKGALFPFYVWMPGAYSAPPSAVL
ALFGGLLTKVGVYSILRTFSLIFRQDIGYTHTILAWLAMATIVLGVIGAVAYRDMRQIAIYNIVAAIGVMAFGISLMTKE
SMEGTIFYLLQDMVMKTALFFIVGAIAYVAGTNQLGRFSGLLGSYPLLGWAVFLTALALSGIPPFSGFIGKALIIRAAFE
KGQLLFALIVLLSSLLVLYSVMKIFIQSCWGEARGYEQKRVAPLYVPIVALLALAVLYGIGAEFVRPYIAQAAATLADPS
LYIESVLKE

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788829, Length=356, Percent_Identity=27.247191011236, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788827, Length=467, Percent_Identity=27.1948608137045, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1788613, Length=352, Percent_Identity=26.7045454545455, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788831, Length=305, Percent_Identity=25.5737704918033, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367154, Length=421, Percent_Identity=22.3277909738717, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693160, Length=247, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53006; Mature: 52875

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAVIFPIAIPLTTAVILIFCVKSLKAQKVISLVSAGALIAAGAWLVRLVDHEGVQKLD
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VGNWPAPFGITLVSDMLSALLVLTTSVIALAGLLYSFYTIDSKQASMYYYVFFQFLIVGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
NGAFSTGDIFNLFVFYEVMLMSSYALLVHGGTKIQLQETMKYMIINVFSSALFVIAVGYL
CCCCCCCHHHHHHHHHHHHHHHCHHHEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YAVTGTLNMAHLAVRISGAENTAVLTVICLLFLIVFGLKGALFPFYVWMPGAYSAPPSAV
HHHHHCCCEEHEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHH
LALFGGLLTKVGVYSILRTFSLIFRQDIGYTHTILAWLAMATIVLGVIGAVAYRDMRQIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYNIVAAIGVMAFGISLMTKESMEGTIFYLLQDMVMKTALFFIVGAIAYVAGTNQLGRFS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLLGSYPLLGWAVFLTALALSGIPPFSGFIGKALIIRAAFEKGQLLFALIVLLSSLLVLY
HHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SVMKIFIQSCWGEARGYEQKRVAPLYVPIVALLALAVLYGIGAEFVRPYIAQAAATLADP
HHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCH
SLYIESVLKE
HHHHHHHHCC
>Mature Secondary Structure 
TNAVIFPIAIPLTTAVILIFCVKSLKAQKVISLVSAGALIAAGAWLVRLVDHEGVQKLD
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VGNWPAPFGITLVSDMLSALLVLTTSVIALAGLLYSFYTIDSKQASMYYYVFFQFLIVGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
NGAFSTGDIFNLFVFYEVMLMSSYALLVHGGTKIQLQETMKYMIINVFSSALFVIAVGYL
CCCCCCCHHHHHHHHHHHHHHHCHHHEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YAVTGTLNMAHLAVRISGAENTAVLTVICLLFLIVFGLKGALFPFYVWMPGAYSAPPSAV
HHHHHCCCEEHEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHH
LALFGGLLTKVGVYSILRTFSLIFRQDIGYTHTILAWLAMATIVLGVIGAVAYRDMRQIA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYNIVAAIGVMAFGISLMTKESMEGTIFYLLQDMVMKTALFFIVGAIAYVAGTNQLGRFS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
GLLGSYPLLGWAVFLTALALSGIPPFSGFIGKALIIRAAFEKGQLLFALIVLLSSLLVLY
HHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SVMKIFIQSCWGEARGYEQKRVAPLYVPIVALLALAVLYGIGAEFVRPYIAQAAATLADP
HHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCH
SLYIESVLKE
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10568751; 10198001; 11356194 [H]