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The map label for this gene is folC [H]

Identifier: 138896203

GI number: 138896203

Start: 2669181

End: 2670485

Strand: Reverse

Name: folC [H]

Synonym: GTNG_2566

Alternate gene names: 138896203

Gene position: 2670485-2669181 (Counterclockwise)

Preceding gene: 138896204

Following gene: 138896202

Centisome position: 75.22

GC content: 52.87

Gene sequence:

>1305_bases
ATGATCCGGACATATGAAGAAGCCGTCACTTGGATTCATGAGCGGCTACGTCTTGGCATGAAGCCGGGGTTGGCCCGGAT
GGAATGGATGATGGAAAAGCTCGGCCATCCGGAACGCCGCGTCCGTGCTGTCCATATCGGCGGAACGAACGGCAAAGGGT
CGACAGTGGCGTATTTGCGTTCGATTTTGCAGGCGGCCGGTTATTCGGTCGGTACGTTCACCTCACCGTACGTTGAGCAA
TTTAACGAACGGATCAGCATCAACGGCGAACCGATTCGCGATGAAGAAATCGTTGAGCTTGTGCAAGTCATTCAACCGCT
CTCGGACGAACTCGAACGAACAGAACTTGGTGGACCGACCGAATTTGAAGTGATCACCGCTATGATGCTCTATTACTTCG
GCAAAAAACGGATTCAAGATATTGTACTTATTGAAGTCGGGCTTGGCGGACGATTGGACTCGACGAATGTGATTTATCCG
CTCGTTTCCGTCATTACGAACGTCAGCTACGACCATATGAACATTTTAGGCGATACGCTCGCGCAAATTGCGGCCGAAAA
AGCAGGAATCATCAAATCGGGGGTTCCGCTTGTGACAGCGGTGAATGAGCCGGAAGCACGGGAGGTCATCGCTAAGACGG
CGGCGGAGCGGAAAGCGAAAACATACTGGCTTGGCGTTGATTTTTCCGTTTCCCACCAGCAAACGACCGCTGAAGGGGAG
CAATTTTCCATCACAACGCCGTTTGCTGAGTATCGTGACTTACGCATTCACATGCCGGGAGCCCACCAAATTGAAAACGC
GGCGACGGCCGTCATGGCGGCTGAATTGCTTCGGCTGAACTATTCGTTTTTGATTGAACCGGAGCACATTCACGACGGAC
TCGCTATGGCTGCTTGGCCGGGCCGGTTTGAGCGGGTGAGCGATGATCCGCTTGTCATTATCGACGGCGCCCATAACGAA
GCCGGTATTCGCGCTCTTGTTGACACGGTGCGCACCCATTACTCGGACAAGCGGGTGCATGTGTTGTTTGCTGCGTTGGC
CGACAAGCCGCTTGAACGAATGATCCGTCTGCTTGACGAGCTGGCTGAGACAATTACGTTTACGACGTTTGATTTCCCAC
GCGCCGCAGCGGCTGATGCACTTGCCGCCCTTTCTTCTCATCCACAGAAAGCGGTCACGGACGATTGGACCGGCTGGATC
CGTGAGAAGAAAAAACAAATAGGGGATGATGAGCTGCTGCTTGTCACCGGATCGCTTTATTTTCTATCGGAAGTGCGGAA
ACTTTTAAAAACCGATGCCAATTGA

Upstream 100 bases:

>100_bases
GACGAATCCGCTATCATGCGGGTTCGTCTTTTTCGCTAGCGAAACACCTAGTCAATCGCTAATTTCTTATATCCATCTTC
TCGAGGGAGGAGTCATCAGC

Downstream 100 bases:

>100_bases
TGAAAAGAATTGATATAATGAAAGAAAAATAGTTCTTTTGGCACGGGAGGGGAGGCGCATGCGGCGACGAAGATGGCTGT
TATGGGGGATGTTGTTCTGG

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIRTYEEAVTWIHERLRLGMKPGLARMEWMMEKLGHPERRVRAVHIGGTNGKGSTVAYLRSILQAAGYSVGTFTSPYVEQ
FNERISINGEPIRDEEIVELVQVIQPLSDELERTELGGPTEFEVITAMMLYYFGKKRIQDIVLIEVGLGGRLDSTNVIYP
LVSVITNVSYDHMNILGDTLAQIAAEKAGIIKSGVPLVTAVNEPEAREVIAKTAAERKAKTYWLGVDFSVSHQQTTAEGE
QFSITTPFAEYRDLRIHMPGAHQIENAATAVMAAELLRLNYSFLIEPEHIHDGLAMAAWPGRFERVSDDPLVIIDGAHNE
AGIRALVDTVRTHYSDKRVHVLFAALADKPLERMIRLLDELAETITFTTFDFPRAAAADALAALSSHPQKAVTDDWTGWI
REKKKQIGDDELLLVTGSLYFLSEVRKLLKTDAN

Sequences:

>Translated_434_residues
MIRTYEEAVTWIHERLRLGMKPGLARMEWMMEKLGHPERRVRAVHIGGTNGKGSTVAYLRSILQAAGYSVGTFTSPYVEQ
FNERISINGEPIRDEEIVELVQVIQPLSDELERTELGGPTEFEVITAMMLYYFGKKRIQDIVLIEVGLGGRLDSTNVIYP
LVSVITNVSYDHMNILGDTLAQIAAEKAGIIKSGVPLVTAVNEPEAREVIAKTAAERKAKTYWLGVDFSVSHQQTTAEGE
QFSITTPFAEYRDLRIHMPGAHQIENAATAVMAAELLRLNYSFLIEPEHIHDGLAMAAWPGRFERVSDDPLVIIDGAHNE
AGIRALVDTVRTHYSDKRVHVLFAALADKPLERMIRLLDELAETITFTTFDFPRAAAADALAALSSHPQKAVTDDWTGWI
REKKKQIGDDELLLVTGSLYFLSEVRKLLKTDAN
>Mature_434_residues
MIRTYEEAVTWIHERLRLGMKPGLARMEWMMEKLGHPERRVRAVHIGGTNGKGSTVAYLRSILQAAGYSVGTFTSPYVEQ
FNERISINGEPIRDEEIVELVQVIQPLSDELERTELGGPTEFEVITAMMLYYFGKKRIQDIVLIEVGLGGRLDSTNVIYP
LVSVITNVSYDHMNILGDTLAQIAAEKAGIIKSGVPLVTAVNEPEAREVIAKTAAERKAKTYWLGVDFSVSHQQTTAEGE
QFSITTPFAEYRDLRIHMPGAHQIENAATAVMAAELLRLNYSFLIEPEHIHDGLAMAAWPGRFERVSDDPLVIIDGAHNE
AGIRALVDTVRTHYSDKRVHVLFAALADKPLERMIRLLDELAETITFTTFDFPRAAAADALAALSSHPQKAVTDDWTGWI
REKKKQIGDDELLLVTGSLYFLSEVRKLLKTDAN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=357, Percent_Identity=30.2521008403361, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI66932984, Length=357, Percent_Identity=30.2521008403361, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1788654, Length=409, Percent_Identity=29.8288508557457, Blast_Score=148, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17553150, Length=347, Percent_Identity=29.6829971181556, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=347, Percent_Identity=29.6829971181556, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=347, Percent_Identity=29.6829971181556, Blast_Score=139, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=435, Percent_Identity=30.3448275862069, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6324815, Length=356, Percent_Identity=30.3370786516854, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322718, Length=295, Percent_Identity=25.4237288135593, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24641571, Length=459, Percent_Identity=26.5795206971678, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24581568, Length=191, Percent_Identity=30.3664921465969, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48455; Mature: 48455

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTYEEAVTWIHERLRLGMKPGLARMEWMMEKLGHPERRVRAVHIGGTNGKGSTVAYLR
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHEEEEEECCCCCCCHHHHHHH
SILQAAGYSVGTFTSPYVEQFNERISINGEPIRDEEIVELVQVIQPLSDELERTELGGPT
HHHHHCCCCCCCCCCHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
EFEVITAMMLYYFGKKRIQDIVLIEVGLGGRLDSTNVIYPLVSVITNVSYDHMNILGDTL
HHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
AQIAAEKAGIIKSGVPLVTAVNEPEAREVIAKTAAERKAKTYWLGVDFSVSHQQTTAEGE
HHHHHHHCCHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHCCCCC
QFSITTPFAEYRDLRIHMPGAHQIENAATAVMAAELLRLNYSFLIEPEHIHDGLAMAAWP
EEEEECCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCEEEECC
GRFERVSDDPLVIIDGAHNEAGIRALVDTVRTHYSDKRVHVLFAALADKPLERMIRLLDE
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
LAETITFTTFDFPRAAAADALAALSSHPQKAVTDDWTGWIREKKKQIGDDELLLVTGSLY
HHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHH
FLSEVRKLLKTDAN
HHHHHHHHHHCCCC
>Mature Secondary Structure
MIRTYEEAVTWIHERLRLGMKPGLARMEWMMEKLGHPERRVRAVHIGGTNGKGSTVAYLR
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHEEEEEECCCCCCCHHHHHHH
SILQAAGYSVGTFTSPYVEQFNERISINGEPIRDEEIVELVQVIQPLSDELERTELGGPT
HHHHHCCCCCCCCCCHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
EFEVITAMMLYYFGKKRIQDIVLIEVGLGGRLDSTNVIYPLVSVITNVSYDHMNILGDTL
HHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
AQIAAEKAGIIKSGVPLVTAVNEPEAREVIAKTAAERKAKTYWLGVDFSVSHQQTTAEGE
HHHHHHHCCHHHCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHCCCCC
QFSITTPFAEYRDLRIHMPGAHQIENAATAVMAAELLRLNYSFLIEPEHIHDGLAMAAWP
EEEEECCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCEEEECC
GRFERVSDDPLVIIDGAHNEAGIRALVDTVRTHYSDKRVHVLFAALADKPLERMIRLLDE
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
LAETITFTTFDFPRAAAADALAALSSHPQKAVTDDWTGWIREKKKQIGDDELLLVTGSLY
HHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHH
FLSEVRKLLKTDAN
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]