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The map label for this gene is pilB [H]

Identifier: 138896196

GI number: 138896196

Start: 2659375

End: 2661039

Strand: Reverse

Name: pilB [H]

Synonym: GTNG_2559

Alternate gene names: 138896196

Gene position: 2661039-2659375 (Counterclockwise)

Preceding gene: 138896197

Following gene: 138896195

Centisome position: 74.95

GC content: 51.05

Gene sequence:

>1665_bases
ATGAGCAAAAAGCAAGAACGGAAACGGCTTGGCGATTTGCTTGTCGAAGCGGGATTAATTACGAAAGAGCAGCTCGCCGA
AGCGCTACGTGAGAGGGTGCCCGGACAAAAGCTTGGCGATGCCTTGTTGCAGCGCGGCTACATTACCGAGCAGCAGCTGA
TTGAAGTGCTTGAGTTCCAGCTTGGCATTCCCCATGTCAGCTTATACCGCTATCCGATCGATCCGAAAGCAACAAGCCTT
GTGCCGAAAGAATTCGCCCGCCGCCATATGGTGATGCCGCTCAAAGTCGAAGGCGAGCGGCTGCTCGTCGCTATGGCTGA
TCCGATGGACTTTTTCGTCATTGACGATCTGCGTCTTTCAACCGGGTTTCAAATCGAGACGGCGATCGCCTCGAAAGACG
ATATTTTAAGAGCCATTAATAAATATTACGACATTGATGAGGTGTTTGCTGATTTTGTGCAGGCATCGCCGCTGGAGGTG
CGCGAAGACGAACAGGCCGATGACGATGATTCCCCGATTGTTCGGTTGGTGAATCAAATTTTGCAGCTTGCCGTTGAGCA
GCGGGCAAGCGATATTCATATCGATCCGCAGGAGACGAAAGTGCTCATTCGCTACCGGATCGATGGTCTCCTCCGCACCG
AGCGGGCGTTGCCGAAACATATGCAAAGCATGTTAACAGCGAGGATTAAAATTTTAGCCAATATGGATATCACGGAACAT
CGCATTCCGCAAGACGGCCGGATGAAAATGGATATCGATTTCCACCCCGTCGATTTGCGTGTTTCCACACTGCCAACGGT
GTACGGCGAGAAGATCGTTATGCGTGTCCTCGATCTAGGGGCAGCCTTGAATGACATTCATAAACTCGGCTTTAATTCGG
TGAATTTGGAGCGTTTCATCCGCTTAATTGAACGGCCAAACGGCATCGTGCTGATCACTGGGCCGACCGGTTCAGGAAAA
TCGTCGACGTTATATGCCGCCTTAAACCATTTAAACCGAGAAGAAGTGAACATCATTACGATTGAGGATCCGGTCGAATA
CCAAATTGAAGGGGTTAACCAAATTCAAGTGAACCCGAACGTCGGCTTGACGTTCGCTCAAGGGCTTCGCTCGATTTTAC
GTCAAGACCCGAACATCATCATGGTCGGGGAAATTCGCGACCGCGAGACGGCCGAAGTGGCGATTCGCGCCTCGCTCACT
GGGCATTTAGTGTTAAGTACGCTTCATACGAACGATGCGTTAAGCACGATCACCCGCTTGATCGACATGGGGATTGAACC
GTTTTTAGTGGCGACATCACTCGTTGGTGTCGTGTCACAGCGGCTCGTGCGCCGCGTCTGTCGCGACTGTCAAGAAACGT
ATGAACCGACAAAGCGGGAGAAAGAAATTTTCGCCCGCCGTGGAATCGAGGTGCAAACACTCGCCCGCGGTCGCGGCTGT
CCGATGTGCAATATGACTGGTTACCGCGGCCGGCTGGCGATTCATGAGCTGCTCGTCGTGACCGATGAGATGCGGCGCGT
CATTTTAAATAATGAACCGTTTTCGAAATTGCGCGAGCTCGCTATTCAAAGCAAAATGATTTTTTTGCTTGATGACGGGC
TATTGAAAGTGAAGCAAGGGATAACGACACTCGAAGAAGTGCTGAAAGTAGCCATTTTGCATTGA

Upstream 100 bases:

>100_bases
ACAAACCGGTGCCAAAAGAAGGCGATGAAGCGGACAGCCGTGAAAATGCGCCTACTGCCAGCGGTAAAGGGGAGACGGAA
GCGAGCGGAGGGGGAGAAAA

Downstream 100 bases:

>100_bases
TAGGTGAGATCCATGAAAGAAAAACTTGAGGCGTTATTACGTGCCGCCTTTGAATGCCGTGCGTCCGATGTGCATTTAAC
TGTCGGCGCACCGCCGATTT

Product: pili biogenesis protein PilB-like ATPase

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSKKQERKRLGDLLVEAGLITKEQLAEALRERVPGQKLGDALLQRGYITEQQLIEVLEFQLGIPHVSLYRYPIDPKATSL
VPKEFARRHMVMPLKVEGERLLVAMADPMDFFVIDDLRLSTGFQIETAIASKDDILRAINKYYDIDEVFADFVQASPLEV
REDEQADDDDSPIVRLVNQILQLAVEQRASDIHIDPQETKVLIRYRIDGLLRTERALPKHMQSMLTARIKILANMDITEH
RIPQDGRMKMDIDFHPVDLRVSTLPTVYGEKIVMRVLDLGAALNDIHKLGFNSVNLERFIRLIERPNGIVLITGPTGSGK
SSTLYAALNHLNREEVNIITIEDPVEYQIEGVNQIQVNPNVGLTFAQGLRSILRQDPNIIMVGEIRDRETAEVAIRASLT
GHLVLSTLHTNDALSTITRLIDMGIEPFLVATSLVGVVSQRLVRRVCRDCQETYEPTKREKEIFARRGIEVQTLARGRGC
PMCNMTGYRGRLAIHELLVVTDEMRRVILNNEPFSKLRELAIQSKMIFLLDDGLLKVKQGITTLEEVLKVAILH

Sequences:

>Translated_554_residues
MSKKQERKRLGDLLVEAGLITKEQLAEALRERVPGQKLGDALLQRGYITEQQLIEVLEFQLGIPHVSLYRYPIDPKATSL
VPKEFARRHMVMPLKVEGERLLVAMADPMDFFVIDDLRLSTGFQIETAIASKDDILRAINKYYDIDEVFADFVQASPLEV
REDEQADDDDSPIVRLVNQILQLAVEQRASDIHIDPQETKVLIRYRIDGLLRTERALPKHMQSMLTARIKILANMDITEH
RIPQDGRMKMDIDFHPVDLRVSTLPTVYGEKIVMRVLDLGAALNDIHKLGFNSVNLERFIRLIERPNGIVLITGPTGSGK
SSTLYAALNHLNREEVNIITIEDPVEYQIEGVNQIQVNPNVGLTFAQGLRSILRQDPNIIMVGEIRDRETAEVAIRASLT
GHLVLSTLHTNDALSTITRLIDMGIEPFLVATSLVGVVSQRLVRRVCRDCQETYEPTKREKEIFARRGIEVQTLARGRGC
PMCNMTGYRGRLAIHELLVVTDEMRRVILNNEPFSKLRELAIQSKMIFLLDDGLLKVKQGITTLEEVLKVAILH
>Mature_553_residues
SKKQERKRLGDLLVEAGLITKEQLAEALRERVPGQKLGDALLQRGYITEQQLIEVLEFQLGIPHVSLYRYPIDPKATSLV
PKEFARRHMVMPLKVEGERLLVAMADPMDFFVIDDLRLSTGFQIETAIASKDDILRAINKYYDIDEVFADFVQASPLEVR
EDEQADDDDSPIVRLVNQILQLAVEQRASDIHIDPQETKVLIRYRIDGLLRTERALPKHMQSMLTARIKILANMDITEHR
IPQDGRMKMDIDFHPVDLRVSTLPTVYGEKIVMRVLDLGAALNDIHKLGFNSVNLERFIRLIERPNGIVLITGPTGSGKS
STLYAALNHLNREEVNIITIEDPVEYQIEGVNQIQVNPNVGLTFAQGLRSILRQDPNIIMVGEIRDRETAEVAIRASLTG
HLVLSTLHTNDALSTITRLIDMGIEPFLVATSLVGVVSQRLVRRVCRDCQETYEPTKREKEIFARRGIEVQTLARGRGCP
MCNMTGYRGRLAIHELLVVTDEMRRVILNNEPFSKLRELAIQSKMIFLLDDGLLKVKQGITTLEEVLKVAILH

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=408, Percent_Identity=47.3039215686275, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786296, Length=479, Percent_Identity=40.5010438413361, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI87082188, Length=277, Percent_Identity=32.8519855595668, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786781, Length=134, Percent_Identity=31.3432835820896, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62777; Mature: 62646

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKQERKRLGDLLVEAGLITKEQLAEALRERVPGQKLGDALLQRGYITEQQLIEVLEFQ
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHH
LGIPHVSLYRYPIDPKATSLVPKEFARRHMVMPLKVEGERLLVAMADPMDFFVIDDLRLS
CCCCCEEEEECCCCCCHHHCCHHHHHHCCCCCEEEECCCEEEEEEECCCCEEEEECEECC
TGFQIETAIASKDDILRAINKYYDIDEVFADFVQASPLEVREDEQADDDDSPIVRLVNQI
CCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
LQLAVEQRASDIHIDPQETKVLIRYRIDGLLRTERALPKHMQSMLTARIKILANMDITEH
HHHHHHHCCCCCCCCHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RIPQDGRMKMDIDFHPVDLRVSTLPTVYGEKIVMRVLDLGAALNDIHKLGFNSVNLERFI
CCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RLIERPNGIVLITGPTGSGKSSTLYAALNHLNREEVNIITIEDPVEYQIEGVNQIQVNPN
HHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEECCCEEEEECCC
VGLTFAQGLRSILRQDPNIIMVGEIRDRETAEVAIRASLTGHLVLSTLHTNDALSTITRL
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHEEEEEECCCEEEEEECCCCHHHHHHHHH
IDMGIEPFLVATSLVGVVSQRLVRRVCRDCQETYEPTKREKEIFARRGIEVQTLARGRGC
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHCCCCC
PMCNMTGYRGRLAIHELLVVTDEMRRVILNNEPFSKLRELAIQSKMIFLLDDGLLKVKQG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHH
ITTLEEVLKVAILH
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SKKQERKRLGDLLVEAGLITKEQLAEALRERVPGQKLGDALLQRGYITEQQLIEVLEFQ
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHH
LGIPHVSLYRYPIDPKATSLVPKEFARRHMVMPLKVEGERLLVAMADPMDFFVIDDLRLS
CCCCCEEEEECCCCCCHHHCCHHHHHHCCCCCEEEECCCEEEEEEECCCCEEEEECEECC
TGFQIETAIASKDDILRAINKYYDIDEVFADFVQASPLEVREDEQADDDDSPIVRLVNQI
CCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
LQLAVEQRASDIHIDPQETKVLIRYRIDGLLRTERALPKHMQSMLTARIKILANMDITEH
HHHHHHHCCCCCCCCHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
RIPQDGRMKMDIDFHPVDLRVSTLPTVYGEKIVMRVLDLGAALNDIHKLGFNSVNLERFI
CCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RLIERPNGIVLITGPTGSGKSSTLYAALNHLNREEVNIITIEDPVEYQIEGVNQIQVNPN
HHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEECCCEEEEECCC
VGLTFAQGLRSILRQDPNIIMVGEIRDRETAEVAIRASLTGHLVLSTLHTNDALSTITRL
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHEEEEEECCCEEEEEECCCCHHHHHHHHH
IDMGIEPFLVATSLVGVVSQRLVRRVCRDCQETYEPTKREKEIFARRGIEVQTLARGRGC
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHCCCCC
PMCNMTGYRGRLAIHELLVVTDEMRRVILNNEPFSKLRELAIQSKMIFLLDDGLLKVKQG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHH
ITTLEEVLKVAILH
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]