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The map label for this gene is narH2 [H]

Identifier: 138895369

GI number: 138895369

Start: 1809995

End: 1811464

Strand: Direct

Name: narH2 [H]

Synonym: GTNG_1713

Alternate gene names: 138895369

Gene position: 1809995-1811464 (Clockwise)

Preceding gene: 138895368

Following gene: 138895370

Centisome position: 50.98

GC content: 52.86

Gene sequence:

>1470_bases
TTGAAGATTAAAGCGCAAGTCGGCATGGTCATGAATTTGGATAAATGTATCGGCTGCCATACGTGCAGCGTCACATGCAA
AAACACATGGACAAACCGGCCCGGCGCCGAATATATGTACTTTAACAACGTGGAAACAAAGCCGGGGATCGGCTATCCGA
AACAGTGGGAAGATCAAGATAAATATAAAGGCGGCTGGGAATTAAAAGACGGCGAATTGCGGCTTAAATCCGGCCCGAAA
GCCATGCGGCTCGTCAATTTGTTCTACAACCCGTACCAGCCGACGATCGACGATTACTACGAACCATGGAACTACGACTA
CGAAACGTTGACGAACAGCCCGCAAAAGAGGTATCAGCCGGTTGCCCGGCCGAAATCGGCCATCACCGGTGAATGGATGG
AAGTATCGTGGGGACCGAACTGGGAAGACGACTTGGCCGGGGCCCATGTCACCGGCTTGCGCGACCCGAACGTTGTCAAA
ATGGAACAGGCGATCCAAGCAGAATTCGAAGATGTCTTTATGATGTATTTGCCGCGCATTTGTGAACATTGTCTCAATCC
GTCATGCGTATCGAGCTGCCCATCGGGCGCTATGTACAAACGCGACGAGGACGGCATCGTCCTCGTCGACCAAAACGCCT
GCCGCGCTTGGCGATATTGCGTCACCAGCTGCCCGTACAAAAAAGTATATTTCAACTGGCAAACGAACAAAGCGGAAAAA
TGCACGCTCTGTTTCCCGCGCATCGAAGCCGGCTTGCCGACAATTTGCTCGGAAACATGTGTCGGCCGCATCCGTTACAT
CGGCGTCATGCTGTATGACGCGGATAAAGTGAAGGAAGCGGCGAGCGTCGCAGATGAGAAACATCTGTATCACGCCCAAC
TTGATATTTTCCTTGACCCAAACGATCCGACTGTCATCGCAGAAGCGAAAAAAGCCGGCATTCCGGACGAATGGATTGCG
GCGGCGCAGCGCTCACCGATTTACAAAATGATCGTTGACTGGAAAATCGCTTTGCCGCTGCACCCGGAATATCGGACACT
GCCAATGGTATGGTACATTCCACCGTTAAGCCCGATCATGAATACGATCGAAGGAAAAGGAAGCGGCGCGAACGCCGATG
ACATCTTCCCAGCCATTGATGAAATGCGCATTCCGATCGAATATTTGGCGAATTTGCTGACGGCCGGCGATACAGCGCAC
ATCCGCACGACATTGAAAAAAATGGCGGCGATGCGTTCGTATATGCGGGCGAAACAGACGAACAAACAGCCGGACATTCG
TCTCATCGAGTCACTCGGCTTAAGCCATGAGGACATCGAAGAGATGTACCGCTTGCTCGCGATCGCCAAATACGAAGATC
GCTTTGTCATCCCGGCGTCACACCGCGAGGAAGTAGCGGATCTCTACACCGAACAAGGCAGCTGTGGGCTCGCATTTGCC
GGCGGTCCGGGCGCTTGCGGCACGCTTTGA

Upstream 100 bases:

>100_bases
GGATACGCGCAGTTAAGCTACGGATTCAACTATTACGGACCGACGGGCAACCAACGCGACTTGTTCGTTGTCATCCGCAA
ATTAGAGGAGGTTGATTGGC

Downstream 100 bases:

>100_bases
TGAGGGCGGGCTTCGCCCCCTTCCCCTCAAACAACGAAAATGGAGGATGCATGATGAACAGCGAACAACGGCAAACCATC
TTTTTATGTGTGTCCTATTT

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQDKYKGGWELKDGELRLKSGPK
AMRLVNLFYNPYQPTIDDYYEPWNYDYETLTNSPQKRYQPVARPKSAITGEWMEVSWGPNWEDDLAGAHVTGLRDPNVVK
MEQAIQAEFEDVFMMYLPRICEHCLNPSCVSSCPSGAMYKRDEDGIVLVDQNACRAWRYCVTSCPYKKVYFNWQTNKAEK
CTLCFPRIEAGLPTICSETCVGRIRYIGVMLYDADKVKEAASVADEKHLYHAQLDIFLDPNDPTVIAEAKKAGIPDEWIA
AAQRSPIYKMIVDWKIALPLHPEYRTLPMVWYIPPLSPIMNTIEGKGSGANADDIFPAIDEMRIPIEYLANLLTAGDTAH
IRTTLKKMAAMRSYMRAKQTNKQPDIRLIESLGLSHEDIEEMYRLLAIAKYEDRFVIPASHREEVADLYTEQGSCGLAFA
GGPGACGTL

Sequences:

>Translated_489_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQDKYKGGWELKDGELRLKSGPK
AMRLVNLFYNPYQPTIDDYYEPWNYDYETLTNSPQKRYQPVARPKSAITGEWMEVSWGPNWEDDLAGAHVTGLRDPNVVK
MEQAIQAEFEDVFMMYLPRICEHCLNPSCVSSCPSGAMYKRDEDGIVLVDQNACRAWRYCVTSCPYKKVYFNWQTNKAEK
CTLCFPRIEAGLPTICSETCVGRIRYIGVMLYDADKVKEAASVADEKHLYHAQLDIFLDPNDPTVIAEAKKAGIPDEWIA
AAQRSPIYKMIVDWKIALPLHPEYRTLPMVWYIPPLSPIMNTIEGKGSGANADDIFPAIDEMRIPIEYLANLLTAGDTAH
IRTTLKKMAAMRSYMRAKQTNKQPDIRLIESLGLSHEDIEEMYRLLAIAKYEDRFVIPASHREEVADLYTEQGSCGLAFA
GGPGACGTL
>Mature_489_residues
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQDKYKGGWELKDGELRLKSGPK
AMRLVNLFYNPYQPTIDDYYEPWNYDYETLTNSPQKRYQPVARPKSAITGEWMEVSWGPNWEDDLAGAHVTGLRDPNVVK
MEQAIQAEFEDVFMMYLPRICEHCLNPSCVSSCPSGAMYKRDEDGIVLVDQNACRAWRYCVTSCPYKKVYFNWQTNKAEK
CTLCFPRIEAGLPTICSETCVGRIRYIGVMLYDADKVKEAASVADEKHLYHAQLDIFLDPNDPTVIAEAKKAGIPDEWIA
AAQRSPIYKMIVDWKIALPLHPEYRTLPMVWYIPPLSPIMNTIEGKGSGANADDIFPAIDEMRIPIEYLANLLTAGDTAH
IRTTLKKMAAMRSYMRAKQTNKQPDIRLIESLGLSHEDIEEMYRLLAIAKYEDRFVIPASHREEVADLYTEQGSCGLAFA
GGPGACGTL

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=485, Percent_Identity=59.5876288659794, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=484, Percent_Identity=59.297520661157, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=37.1212121212121, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=37.1212121212121, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI2367345, Length=136, Percent_Identity=30.1470588235294, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=39.5348837209302, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789370, Length=103, Percent_Identity=33.9805825242718, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790326, Length=135, Percent_Identity=28.8888888888889, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 55469; Mature: 55469

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQD
CEECCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCH
KYKGGWELKDGELRLKSGPKAMRLVNLFYNPYQPTIDDYYEPWNYDYETLTNSPQKRYQP
HCCCCCEECCCCEEECCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHCCCCHHHCCC
VARPKSAITGEWMEVSWGPNWEDDLAGAHVTGLRDPNVVKMEQAIQAEFEDVFMMYLPRI
CCCCCHHCCCCEEEECCCCCCCHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CEHCLNPSCVSSCPSGAMYKRDEDGIVLVDQNACRAWRYCVTSCPYKKVYFNWQTNKAEK
HHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCE
CTLCFPRIEAGLPTICSETCVGRIRYIGVMLYDADKVKEAASVADEKHLYHAQLDIFLDP
EEEECHHHHCCCCHHHHHHHHHHEEEEEEEEECHHHHHHHHHHHHHHCEEEEEEEEEECC
NDPTVIAEAKKAGIPDEWIAAAQRSPIYKMIVDWKIALPLHPEYRTLPMVWYIPPLSPIM
CCCEEEEEHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHHH
NTIEGKGSGANADDIFPAIDEMRIPIEYLANLLTAGDTAHIRTTLKKMAAMRSYMRAKQT
HHHCCCCCCCCCHHHCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
NKQPDIRLIESLGLSHEDIEEMYRLLAIAKYEDRFVIPASHREEVADLYTEQGSCGLAFA
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCEEEEC
GGPGACGTL
CCCCCCCCC
>Mature Secondary Structure
MKIKAQVGMVMNLDKCIGCHTCSVTCKNTWTNRPGAEYMYFNNVETKPGIGYPKQWEDQD
CEECCCCCCEEEHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCH
KYKGGWELKDGELRLKSGPKAMRLVNLFYNPYQPTIDDYYEPWNYDYETLTNSPQKRYQP
HCCCCCEECCCCEEECCCCHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHCCCCHHHCCC
VARPKSAITGEWMEVSWGPNWEDDLAGAHVTGLRDPNVVKMEQAIQAEFEDVFMMYLPRI
CCCCCHHCCCCEEEECCCCCCCHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
CEHCLNPSCVSSCPSGAMYKRDEDGIVLVDQNACRAWRYCVTSCPYKKVYFNWQTNKAEK
HHHHCCCHHHHCCCCCCCEEECCCCEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCE
CTLCFPRIEAGLPTICSETCVGRIRYIGVMLYDADKVKEAASVADEKHLYHAQLDIFLDP
EEEECHHHHCCCCHHHHHHHHHHEEEEEEEEECHHHHHHHHHHHHHHCEEEEEEEEEECC
NDPTVIAEAKKAGIPDEWIAAAQRSPIYKMIVDWKIALPLHPEYRTLPMVWYIPPLSPIM
CCCEEEEEHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEEECCHHHHH
NTIEGKGSGANADDIFPAIDEMRIPIEYLANLLTAGDTAHIRTTLKKMAAMRSYMRAKQT
HHHCCCCCCCCCHHHCCCHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
NKQPDIRLIESLGLSHEDIEEMYRLLAIAKYEDRFVIPASHREEVADLYTEQGSCGLAFA
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCEEEEC
GGPGACGTL
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]