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The map label for this gene is kinB [H]

Identifier: 138895124

GI number: 138895124

Start: 1543831

End: 1545123

Strand: Reverse

Name: kinB [H]

Synonym: GTNG_1462

Alternate gene names: 138895124

Gene position: 1545123-1543831 (Counterclockwise)

Preceding gene: 138895125

Following gene: 138895121

Centisome position: 43.52

GC content: 50.73

Gene sequence:

>1293_bases
ATGGCAGGTTTATATATTAATCAACACGTCCTCAATAATTTGTTTTATATTTTAGTCACTGTGTTTGCGTTTTCATTCAT
TTATGACCATAGCCCAGCAATTCAGCGCAAACCGCTGTACAACCGGACGCTTCTTTGCGCCTGCTTGGCGCTGGCGTCTG
TGCTGTGCATGAGATTTCCCATTTATATTGATCCGACGTGCATCCACGATTTCCGGCAAATTCCGTTTTTGCTTGGAACG
CTCTATGGCGGCGGTATCGTTGGAGCCGTGTTTTTTGCTGTGTTAATGCTGGCACGCACCATCTTGTACGGGTTTCAATC
GTTGACATTCGTCGTCTATGCCATCATGTTCGTTGTTGCGGCGGCCGCCTCTCCACTATTCCGCCGTCTACCTCGGTCTG
GCAAGCTGTTCGCTTCGCTTTGGCTGACGTTTTTGATTGCGATCTTAACAACATTTGTGGCCGTCATTATCGCTGATTTT
TCGGTGACGAAGCCGTATATCGTGTATTTTATTATTATGCCGCCATTTGTCATGATGTTCGCCGTCTATTTGATGGAAAC
GCTCCATGAGGCGCAGCTCGCTCGGGCGGAGCTGATGAAGATGGAAAAAATGGAGATCGTCAGCCAGCTGGCTGCCAGCA
TTTCGCACGAAATCCGCAACCCTCTCACTGTCGTGAAAGGGTTCATTCAGCTGCTGCAAACCGAGCCGCTGCCGCGCGAA
ACAAAGGAGCGGTACATCCGCATCGCCCTAGAGGAAGTCGAGCGGGCGGAAGGGATCATCCATGATTATTTAACGTTTGC
CAAACCAGCACCGAAAGAGACCGAACCGATCGATGTGGCCGTCGAGCTTCGCAAGGTGTTGCAAATGATCGCACCGATGG
CGCATATGCATTCGGTCGATCTCTCTTCTTCCCTTGAACACGGCATCGTCATTGGGAATGTGCAATATTTCCAACAGTGT
TTCCTCAATTTGTTAAAAAACAGCATCGAAGCGATGCCAAACGGCGGCACGCTCCGTGTGACGGTCGAGAACCAAGAAAC
ATCCGTCGTCATCACCGTTTCCGACAACGGTATCGGCATGACGAAAGAACAAGTGCGCCGCTTCGGTGAACCGTATTTCA
GCACGAAAGAGAAGGGAACCGGGCTTGGCGCCATGGTCGCAGTAAAAATCATCGAACAAATGGGCGGGACGTGGAAAGTC
GAGAGCACCGTCCAAAAAGGAACGACGTTGACGATCACCTTGCCGGCACGCCAGCTGCGGCTATTATCGTCACAACAGAG
CGCCAATAGCTGA

Upstream 100 bases:

>100_bases
GGCTGCCGAAAGACCAGAGGCAACAAAACTATTTTTCTTTCAACCGCTTTCCGATTATAATGGAAGACAATGATGTTCAC
CCGATTAGGAGATTTTTTGT

Downstream 100 bases:

>100_bases
ACAAAAAACGGATGTCCGCCTTCAGACATCCGTTTTTTATGGCCGCTTGCCCCGTCACTTGATTTCCTCAAAATAATCTT
TGTAAAACCCGCCGACTTTT

Product: sporulation kinase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAGLYINQHVLNNLFYILVTVFAFSFIYDHSPAIQRKPLYNRTLLCACLALASVLCMRFPIYIDPTCIHDFRQIPFLLGT
LYGGGIVGAVFFAVLMLARTILYGFQSLTFVVYAIMFVVAAAASPLFRRLPRSGKLFASLWLTFLIAILTTFVAVIIADF
SVTKPYIVYFIIMPPFVMMFAVYLMETLHEAQLARAELMKMEKMEIVSQLAASISHEIRNPLTVVKGFIQLLQTEPLPRE
TKERYIRIALEEVERAEGIIHDYLTFAKPAPKETEPIDVAVELRKVLQMIAPMAHMHSVDLSSSLEHGIVIGNVQYFQQC
FLNLLKNSIEAMPNGGTLRVTVENQETSVVITVSDNGIGMTKEQVRRFGEPYFSTKEKGTGLGAMVAVKIIEQMGGTWKV
ESTVQKGTTLTITLPARQLRLLSSQQSANS

Sequences:

>Translated_430_residues
MAGLYINQHVLNNLFYILVTVFAFSFIYDHSPAIQRKPLYNRTLLCACLALASVLCMRFPIYIDPTCIHDFRQIPFLLGT
LYGGGIVGAVFFAVLMLARTILYGFQSLTFVVYAIMFVVAAAASPLFRRLPRSGKLFASLWLTFLIAILTTFVAVIIADF
SVTKPYIVYFIIMPPFVMMFAVYLMETLHEAQLARAELMKMEKMEIVSQLAASISHEIRNPLTVVKGFIQLLQTEPLPRE
TKERYIRIALEEVERAEGIIHDYLTFAKPAPKETEPIDVAVELRKVLQMIAPMAHMHSVDLSSSLEHGIVIGNVQYFQQC
FLNLLKNSIEAMPNGGTLRVTVENQETSVVITVSDNGIGMTKEQVRRFGEPYFSTKEKGTGLGAMVAVKIIEQMGGTWKV
ESTVQKGTTLTITLPARQLRLLSSQQSANS
>Mature_429_residues
AGLYINQHVLNNLFYILVTVFAFSFIYDHSPAIQRKPLYNRTLLCACLALASVLCMRFPIYIDPTCIHDFRQIPFLLGTL
YGGGIVGAVFFAVLMLARTILYGFQSLTFVVYAIMFVVAAAASPLFRRLPRSGKLFASLWLTFLIAILTTFVAVIIADFS
VTKPYIVYFIIMPPFVMMFAVYLMETLHEAQLARAELMKMEKMEIVSQLAASISHEIRNPLTVVKGFIQLLQTEPLPRET
KERYIRIALEEVERAEGIIHDYLTFAKPAPKETEPIDVAVELRKVLQMIAPMAHMHSVDLSSSLEHGIVIGNVQYFQQCF
LNLLKNSIEAMPNGGTLRVTVENQETSVVITVSDNGIGMTKEQVRRFGEPYFSTKEKGTGLGAMVAVKIIEQMGGTWKVE
STVQKGTTLTITLPARQLRLLSSQQSANS

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. Spo0F is required for the kinB activity [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=275, Percent_Identity=29.0909090909091, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788549, Length=244, Percent_Identity=25.8196721311475, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=28.110599078341, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788713, Length=224, Percent_Identity=28.125, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789149, Length=254, Percent_Identity=30.3149606299213, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI145693157, Length=250, Percent_Identity=28, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790300, Length=238, Percent_Identity=28.1512605042017, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081816, Length=246, Percent_Identity=26.0162601626016, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=243, Percent_Identity=24.6913580246914, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48154; Mature: 48022

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCC
>Mature Secondary Structure 
AGLYINQHVLNNLFYILVTVFAFSFIYDHSPAIQRKPLYNRTLLCACLALASVLCMRFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC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HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]