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The map label for this gene is mcrB [C]

Identifier: 138894900

GI number: 138894900

Start: 1302185

End: 1303237

Strand: Direct

Name: mcrB [C]

Synonym: GTNG_1238

Alternate gene names: 138894900

Gene position: 1302185-1303237 (Clockwise)

Preceding gene: 138894899

Following gene: 138894901

Centisome position: 36.68

GC content: 50.43

Gene sequence:

>1053_bases
ATGGACAAAAAGGTGACGGATTCTACACAGCGCGGCTATTATATCGTCTACTTGTTCAGCGAGGATATGTGTCGGCTCTA
TTTGACGCTCACCCAAAGAGTGACAGAAACGCCGCGAGATGAAATGGAGCGGGTGAAGCAGGATATCCGTCAGCAGATTC
CTGTTGGGGGTCGCGTGCAAACTGATAACGCCATCCGTCTCAGTGCAAGCAAACGGGCGAAAGAATATGAGCGGTCAGTG
GCGGTGTATATTGCCTATTCGTTTGACAATCTGCCGTCCGATGAACAACTCGTGAGCGACTTGAAAACGATGATCGGCTA
TTACCGACAATACGTGGAACGAACAGAAAAGATGACGCCGTTTAAGCAATCGCTCTCCGATCGGTCAGTCGTCGAACATA
TTCATTTCTACATAACCGCTAAAGGATTTTACTATACGCAAGAAGAAGTGATGAATCTTTTTCTTTCACTAAAAACAAAA
CCGTTTGTCATTTTGTCCGGCATTTCCGGAACAGGGAAAACGAAAATCGCCCAATGGCTGGCTGAAAGCGTCGGTGCGAC
GGAAGACAACGGGCAGTTCATCTTAATTCCCGTTTGCCCAGATTGGAACGATGGCTCTGACTTGCTTGGCTATGTCGACA
TTAAGGGCGATTTCAAGCCTGGGCCGCTCACCAATGTCATTACCGAAGCCGAGAACCATCCGGACAAGCCGTATTTCGTT
GTGCTGGATGAAATGAACCTCGCTCGCGTCGAGCATTATTTCAGCGACGTCTTAAGCGTGATGGGAAGCCGCCGTTGGGA
AAATGGCCGCATTACCTTGTCGCGGCTACTGCCGAAAGAAACGGCGGGGCGTGATCTCTTTCTCCCGCCAAACGTCTATA
TCATCGGCACCGTCAACATGGACGAAACAACCCACCCCTTCAGCAAAAAAGTGCTTGATCGGGCGAATACGATCGAGTTC
AATCGCATCCGGCTCGACCATCTTGACTTTTTGCGCGACTTGCCAACCGTCGCGCCGCTGTCAGGCGGACAAGAATGGTT
TGCGGCTCGGTAG

Upstream 100 bases:

>100_bases
GGAAAAGATAAAAGCGCTGCCGTTTGTTGAGCCCGACCGATATATCGTCAAAGGTTCAGTCGGCCAAGGAGTGTGGACCG
ATGTGCCGTAGGTGGAGGTG

Downstream 100 bases:

>100_bases
TCCGGTCGGCTACGGCATCGCCGTCTATGCGATGACGACGCTTAATACAATCAAACAGGCAAAAGAGCTGCCCGAGCTGT
TCATGAAATCAAGCGAGGAA

Product: 5-methylcytosine-specific restriction related enzyme

Products: NA

Alternate protein names: GTPase Subunit Of Restriction Endonuclease-Like Protein; 5-Methylcytosine-Specific Restriction ; GTPase Subunit Of Restriction Endonuclease-Like; Site-Specific DNA-Methyltransferase Cytosine-Specific; 5-Methylcytosine-Specific Restriction McrB Related Protein; 5-Methylcytosine-Specific Restriction-Like Protein; AAA Family ATPase; AAA ATPase; ATPase AAA Family

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDKKVTDSTQRGYYIVYLFSEDMCRLYLTLTQRVTETPRDEMERVKQDIRQQIPVGGRVQTDNAIRLSASKRAKEYERSV
AVYIAYSFDNLPSDEQLVSDLKTMIGYYRQYVERTEKMTPFKQSLSDRSVVEHIHFYITAKGFYYTQEEVMNLFLSLKTK
PFVILSGISGTGKTKIAQWLAESVGATEDNGQFILIPVCPDWNDGSDLLGYVDIKGDFKPGPLTNVITEAENHPDKPYFV
VLDEMNLARVEHYFSDVLSVMGSRRWENGRITLSRLLPKETAGRDLFLPPNVYIIGTVNMDETTHPFSKKVLDRANTIEF
NRIRLDHLDFLRDLPTVAPLSGGQEWFAAR

Sequences:

>Translated_350_residues
MDKKVTDSTQRGYYIVYLFSEDMCRLYLTLTQRVTETPRDEMERVKQDIRQQIPVGGRVQTDNAIRLSASKRAKEYERSV
AVYIAYSFDNLPSDEQLVSDLKTMIGYYRQYVERTEKMTPFKQSLSDRSVVEHIHFYITAKGFYYTQEEVMNLFLSLKTK
PFVILSGISGTGKTKIAQWLAESVGATEDNGQFILIPVCPDWNDGSDLLGYVDIKGDFKPGPLTNVITEAENHPDKPYFV
VLDEMNLARVEHYFSDVLSVMGSRRWENGRITLSRLLPKETAGRDLFLPPNVYIIGTVNMDETTHPFSKKVLDRANTIEF
NRIRLDHLDFLRDLPTVAPLSGGQEWFAAR
>Mature_350_residues
MDKKVTDSTQRGYYIVYLFSEDMCRLYLTLTQRVTETPRDEMERVKQDIRQQIPVGGRVQTDNAIRLSASKRAKEYERSV
AVYIAYSFDNLPSDEQLVSDLKTMIGYYRQYVERTEKMTPFKQSLSDRSVVEHIHFYITAKGFYYTQEEVMNLFLSLKTK
PFVILSGISGTGKTKIAQWLAESVGATEDNGQFILIPVCPDWNDGSDLLGYVDIKGDFKPGPLTNVITEAENHPDKPYFV
VLDEMNLARVEHYFSDVLSVMGSRRWENGRITLSRLLPKETAGRDLFLPPNVYIIGTVNMDETTHPFSKKVLDRANTIEF
NRIRLDHLDFLRDLPTVAPLSGGQEWFAAR

Specific function: Recognizes N4- And C5-Methylcytosine (And 5-Hydroxy- Methylcytosines) Produced By A Broad Range Of DNA Methylases And Appears To Act Against 5-Methylcytosine Preceded By A Purine Residue. Binds To DNA Containing Methylated Cytosines; Also Binds To GTP. [

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.21.- [C]

Molecular weight: Translated: 40215; Mature: 40215

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKVTDSTQRGYYIVYLFSEDMCRLYLTLTQRVTETPRDEMERVKQDIRQQIPVGGRVQ
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEE
TDNAIRLSASKRAKEYERSVAVYIAYSFDNLPSDEQLVSDLKTMIGYYRQYVERTEKMTP
CCCEEEECHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
FKQSLSDRSVVEHIHFYITAKGFYYTQEEVMNLFLSLKTKPFVILSGISGTGKTKIAQWL
HHHHCCHHHHHHEEEEEEEECCEEECHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
AESVGATEDNGQFILIPVCPDWNDGSDLLGYVDIKGDFKPGPLTNVITEAENHPDKPYFV
HHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEE
VLDEMNLARVEHYFSDVLSVMGSRRWENGRITLSRLLPKETAGRDLFLPPNVYIIGTVNM
EEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCHHHCCCEEEECCCEEEEEEECC
DETTHPFSKKVLDRANTIEFNRIRLDHLDFLRDLPTVAPLSGGQEWFAAR
CCCCCHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MDKKVTDSTQRGYYIVYLFSEDMCRLYLTLTQRVTETPRDEMERVKQDIRQQIPVGGRVQ
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEE
TDNAIRLSASKRAKEYERSVAVYIAYSFDNLPSDEQLVSDLKTMIGYYRQYVERTEKMTP
CCCEEEECHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
FKQSLSDRSVVEHIHFYITAKGFYYTQEEVMNLFLSLKTKPFVILSGISGTGKTKIAQWL
HHHHCCHHHHHHEEEEEEEECCEEECHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
AESVGATEDNGQFILIPVCPDWNDGSDLLGYVDIKGDFKPGPLTNVITEAENHPDKPYFV
HHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEE
VLDEMNLARVEHYFSDVLSVMGSRRWENGRITLSRLLPKETAGRDLFLPPNVYIIGTVNM
EEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCHHHCCCEEEECCCEEEEEEECC
DETTHPFSKKVLDRANTIEFNRIRLDHLDFLRDLPTVAPLSGGQEWFAAR
CCCCCHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA