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The map label for this gene is psuK [H]

Identifier: 138894895

GI number: 138894895

Start: 1297989

End: 1298954

Strand: Direct

Name: psuK [H]

Synonym: GTNG_1231

Alternate gene names: 138894895

Gene position: 1297989-1298954 (Clockwise)

Preceding gene: 138894894

Following gene: 138894896

Centisome position: 36.56

GC content: 56.11

Gene sequence:

>966_bases
ATGGATGCGATGGAAAGGATGAGAGACATGCGAACGAAAGGTTCCATCGTCTGCATCGGCGGGGTGAACGTCGATCGGAA
AGCGCGGCTGCTTGTGCCGTTTGAGCTCGGCACGTCCCATCCTGTTGTGAGCACGCAAACAGCAGGTGGGGTGGCGAGAA
ACATCGCCGAAAATATCGGTAGGCTCGGGCAAAACGTTGCGCTCCTCAGCGTAGTCGGTGGCGACCATGACGGTCAATGG
CTCATTGATGCCACGAGTCCATATGTTGACACAAGGATGGTCACACGAATACCGGAAGCGAACACCGGCGCATATACGGC
GGTGCTCGATGAGCGTGGCGAGATGGTACTCGCCTTGGCCGATATGGCGATTTATGATACAGTAAGGAACGAATGGCTGA
AGCAGCGATGGTTGAAATTCGGTCCGATTTCTGCTGTCGTGCTCGATACGAATTTTCCGACTGATGTCATCTCATGGGTG
ATCGAGCAGTGCCGCCAAGACCGCCTTCCGCTTTGCGTCGTGACTGTGTCCGTGCCCAAAGTGAAGCGGTTGCCGACGGT
TCTTTCCGGTGTCACTTGGCTTGTCACGAATCAGGCGGAAGCCATGGCGCTTGCGGGGAAACGCCGCCTTGATGATGCGA
TCGAATCGCTTTTTCGTTTGGGAGTGGAAAATATCGTCATCACGCGCGGGGCTGAGGGCGTCGTGTACGCGACAAGGAAA
GGGGAGAGGGGAGCGATTGCAGCTCCTGTGGTCAAGGTTGCCGATGCTACCGGGGCGGGCGATGCGTTCGCAGCTGGATT
TTTGTATGGCGTCTTGAACGGACATATGGTGGAAGACGCCTGCCGCCTCGGGATAAGCAACGCGGCTTTGACGCTGCAAA
CCGCCGAAACGGTAAATCCCGCTTTGACGGAACAACGGTTGCGAGCGGCGTATGAACAGTATTTTCACGAGGGAGGAGAA
TCATAA

Upstream 100 bases:

>100_bases
CTAGCAATCTTGCATCCCGCTTATACGCGGGATGCGTTTTTCATTTGTAGGGGCTGTATGATACAATAGTAAAAATTGGA
AGCAGAGGCGTCATTTTCGA

Downstream 100 bases:

>100_bases
TGAACGACATCCTTGTTTTTTCTGAAGAAGTGGCCGCAGCGAAAGCGGAACGCAAGCCAATCGTCGCTTTGGAGTCAACG
ATCATTTCCCACGGCATGCC

Product: ribokinase family sugar kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDAMERMRDMRTKGSIVCIGGVNVDRKARLLVPFELGTSHPVVSTQTAGGVARNIAENIGRLGQNVALLSVVGGDHDGQW
LIDATSPYVDTRMVTRIPEANTGAYTAVLDERGEMVLALADMAIYDTVRNEWLKQRWLKFGPISAVVLDTNFPTDVISWV
IEQCRQDRLPLCVVTVSVPKVKRLPTVLSGVTWLVTNQAEAMALAGKRRLDDAIESLFRLGVENIVITRGAEGVVYATRK
GERGAIAAPVVKVADATGAGDAFAAGFLYGVLNGHMVEDACRLGISNAALTLQTAETVNPALTEQRLRAAYEQYFHEGGE
S

Sequences:

>Translated_321_residues
MDAMERMRDMRTKGSIVCIGGVNVDRKARLLVPFELGTSHPVVSTQTAGGVARNIAENIGRLGQNVALLSVVGGDHDGQW
LIDATSPYVDTRMVTRIPEANTGAYTAVLDERGEMVLALADMAIYDTVRNEWLKQRWLKFGPISAVVLDTNFPTDVISWV
IEQCRQDRLPLCVVTVSVPKVKRLPTVLSGVTWLVTNQAEAMALAGKRRLDDAIESLFRLGVENIVITRGAEGVVYATRK
GERGAIAAPVVKVADATGAGDAFAAGFLYGVLNGHMVEDACRLGISNAALTLQTAETVNPALTEQRLRAAYEQYFHEGGE
S
>Mature_321_residues
MDAMERMRDMRTKGSIVCIGGVNVDRKARLLVPFELGTSHPVVSTQTAGGVARNIAENIGRLGQNVALLSVVGGDHDGQW
LIDATSPYVDTRMVTRIPEANTGAYTAVLDERGEMVLALADMAIYDTVRNEWLKQRWLKFGPISAVVLDTNFPTDVISWV
IEQCRQDRLPLCVVTVSVPKVKRLPTVLSGVTWLVTNQAEAMALAGKRRLDDAIESLFRLGVENIVITRGAEGVVYATRK
GERGAIAAPVVKVADATGAGDAFAAGFLYGVLNGHMVEDACRLGISNAALTLQTAETVNPALTEQRLRAAYEQYFHEGGE
S

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=302, Percent_Identity=30.794701986755, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788484, Length=315, Percent_Identity=28.2539682539683, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081971, Length=323, Percent_Identity=27.2445820433437, Blast_Score=83, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24580669, Length=326, Percent_Identity=24.5398773006135, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 34711; Mature: 34711

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAMERMRDMRTKGSIVCIGGVNVDRKARLLVPFELGTSHPVVSTQTAGGVARNIAENIG
CCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHH
RLGQNVALLSVVGGDHDGQWLIDATSPYVDTRMVTRIPEANTGAYTAVLDERGEMVLALA
HHCCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEEECCCCCEEEEEH
DMAIYDTVRNEWLKQRWLKFGPISAVVLDTNFPTDVISWVIEQCRQDRLPLCVVTVSVPK
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCC
VKRLPTVLSGVTWLVTNQAEAMALAGKRRLDDAIESLFRLGVENIVITRGAEGVVYATRK
HHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECC
GERGAIAAPVVKVADATGAGDAFAAGFLYGVLNGHMVEDACRLGISNAALTLQTAETVNP
CCCCCCHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEHHHCCH
ALTEQRLRAAYEQYFHEGGES
HHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDAMERMRDMRTKGSIVCIGGVNVDRKARLLVPFELGTSHPVVSTQTAGGVARNIAENIG
CCHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCCHHHHHHHHHHH
RLGQNVALLSVVGGDHDGQWLIDATSPYVDTRMVTRIPEANTGAYTAVLDERGEMVLALA
HHCCCEEEEEEECCCCCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEEECCCCCEEEEEH
DMAIYDTVRNEWLKQRWLKFGPISAVVLDTNFPTDVISWVIEQCRQDRLPLCVVTVSVPK
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCC
VKRLPTVLSGVTWLVTNQAEAMALAGKRRLDDAIESLFRLGVENIVITRGAEGVVYATRK
HHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECC
GERGAIAAPVVKVADATGAGDAFAAGFLYGVLNGHMVEDACRLGISNAALTLQTAETVNP
CCCCCCHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEHHHCCH
ALTEQRLRAAYEQYFHEGGES
HHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]