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The map label for this gene is sigG [H]

Identifier: 138894663

GI number: 138894663

Start: 1059933

End: 1060727

Strand: Direct

Name: sigG [H]

Synonym: GTNG_0993

Alternate gene names: 138894663

Gene position: 1059933-1060727 (Clockwise)

Preceding gene: 138894662

Following gene: 138894664

Centisome position: 29.85

GC content: 46.67

Gene sequence:

>795_bases
ATGGGAGGGAACAACTTGACGAGGAACAAAGTCGAGATTTGCGGTGTGGATACTTCAAAGCTTCCCGTCCTCAAAAATGA
GGAAATGCGTGAACTGTTTCAACGGATGCATGAAGGGGATTTAGAGGCGAGAGAAAAATTAGTAAACGGCAATTTGCGCC
TTGTGTTGAGCGTCATCCAACGCTTTAACAACCGTGGCGAATTTGTTGATGATTTGTTTCAAGTCGGTTGCATCGGACTT
ATGAAATCGATTGATAATTTTGATTTAAATCAAAATGTTAAGTTTTCCACGTATGCGGTGCCGATGATTATCGGCGAAAT
CCGCCGCTATTTGCGTGACAACAATCCGATCCGCGTTTCGCGCTCGCTGCGCGATATTGCCTACAAAGCGTTGCAAGTGC
GAGAACGGCTTATGGGTGAGACAGCGAAAGAGCCGTCGACGGAAGAGATTGCGAAAGAACTTGGTGTCGCCCATGAAGAA
GTCGTTTTGGCCCTCGATGCCATTCAAGATCCGGTTTCGCTATTTGAACCGATTTACAATGACGGCGGCGATCCGATTTA
CGTGATGGATCAGCTGAGCGATGAGCGCAACCGCGACAGTCAATGGATTGAAGAAATCGCATTGAAAGAAGGGTTGCGGC
GGTTAAATGAGCGGGAAAAAATGATCATCCGCAAACGATTTTTCCAAGGGAAAACACAAATGGAGGTCGCTGAGGAAATC
GGTATTTCCCAAGCGCAAGTATCGCGGTTGGAAAAAGCAGCGATCCGGCAAATGAATAAAAACATCCAAGTGTGA

Upstream 100 bases:

>100_bases
GCAGGGGCAGATGCTCTGCCGCTTTTTTTCAGCCCGGACAGGCTGTGCATATTTTTCCCTCCTAAGGAGATACTGAAAAC
TGACGACTTAGCATCTCCTG

Downstream 100 bases:

>100_bases
GCGGCTGCTAGAGCAGCCTTTTTTCTTTGTTACATAACCTGTTTGGCGGCGTCATATAGATGAAAAAGAGGAGAGGAAAG
GCGGGGGAATGAACCGTGAT

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGGNNLTRNKVEICGVDTSKLPVLKNEEMRELFQRMHEGDLEAREKLVNGNLRLVLSVIQRFNNRGEFVDDLFQVGCIGL
MKSIDNFDLNQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLMGETAKEPSTEEIAKELGVAHEE
VVLALDAIQDPVSLFEPIYNDGGDPIYVMDQLSDERNRDSQWIEEIALKEGLRRLNEREKMIIRKRFFQGKTQMEVAEEI
GISQAQVSRLEKAAIRQMNKNIQV

Sequences:

>Translated_264_residues
MGGNNLTRNKVEICGVDTSKLPVLKNEEMRELFQRMHEGDLEAREKLVNGNLRLVLSVIQRFNNRGEFVDDLFQVGCIGL
MKSIDNFDLNQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLMGETAKEPSTEEIAKELGVAHEE
VVLALDAIQDPVSLFEPIYNDGGDPIYVMDQLSDERNRDSQWIEEIALKEGLRRLNEREKMIIRKRFFQGKTQMEVAEEI
GISQAQVSRLEKAAIRQMNKNIQV
>Mature_263_residues
GGNNLTRNKVEICGVDTSKLPVLKNEEMRELFQRMHEGDLEAREKLVNGNLRLVLSVIQRFNNRGEFVDDLFQVGCIGLM
KSIDNFDLNQNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLMGETAKEPSTEEIAKELGVAHEEV
VLALDAIQDPVSLFEPIYNDGGDPIYVMDQLSDERNRDSQWIEEIALKEGLRRLNEREKMIIRKRFFQGKTQMEVAEEIG
ISQAQVSRLEKAAIRQMNKNIQV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=248, Percent_Identity=29.4354838709677, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1789871, Length=264, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789098, Length=258, Percent_Identity=27.1317829457364, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30425; Mature: 30294

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGNNLTRNKVEICGVDTSKLPVLKNEEMRELFQRMHEGDLEAREKLVNGNLRLVLSVIQ
CCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
RFNNRGEFVDDLFQVGCIGLMKSIDNFDLNQNVKFSTYAVPMIIGEIRRYLRDNNPIRVS
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHH
RSLRDIAYKALQVRERLMGETAKEPSTEEIAKELGVAHEEVVLALDAIQDPVSLFEPIYN
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHC
DGGDPIYVMDQLSDERNRDSQWIEEIALKEGLRRLNEREKMIIRKRFFQGKTQMEVAEEI
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GISQAQVSRLEKAAIRQMNKNIQV
CCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GGNNLTRNKVEICGVDTSKLPVLKNEEMRELFQRMHEGDLEAREKLVNGNLRLVLSVIQ
CCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHH
RFNNRGEFVDDLFQVGCIGLMKSIDNFDLNQNVKFSTYAVPMIIGEIRRYLRDNNPIRVS
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHH
RSLRDIAYKALQVRERLMGETAKEPSTEEIAKELGVAHEEVVLALDAIQDPVSLFEPIYN
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHC
DGGDPIYVMDQLSDERNRDSQWIEEIALKEGLRRLNEREKMIIRKRFFQGKTQMEVAEEI
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GISQAQVSRLEKAAIRQMNKNIQV
CCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377 [H]