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The map label for this gene is sigE [H]

Identifier: 138894662

GI number: 138894662

Start: 1059107

End: 1059826

Strand: Direct

Name: sigE [H]

Synonym: GTNG_0992

Alternate gene names: 138894662

Gene position: 1059107-1059826 (Clockwise)

Preceding gene: 138894661

Following gene: 138894663

Centisome position: 29.83

GC content: 47.78

Gene sequence:

>720_bases
ATGAAAAAGTGGAAACTTCGCTTCATGTATTGGGTGTATAAGCTTCTGGCGAAACTCGGTTTGAAGACGGATGAGATTTA
TTATATCGGCGGCAGTGAAGCGCTCCCTCCGCCGCTGACGAAAGAGGAAGAGGAACGGCTGATCGAGCGGTTGGCGGTCG
GTGATAAGACAGCGCGGTCGCTTCTCATCGAGCGCAACTTACGCCTTGTCGTTTACATCGCGCGCAAATTTGAAAACACC
GGCATTCATATCGAGGACTTGATCAGCATTGGAACGATCGGACTGATTAAGGCGGTCAACACGTTCAATCCAGAGAAAAA
AATCAAGCTGGCGACGTATGCTTCACGCTGCATTGAAAATGAAATTTTAATGTATTTGCGCCGCAATAATAAAGTGCGGG
CGGAAGTGTCATTTGATGAGCCGTTGAATATTGACTGGGACGGCAATGAGTTGCTGCTGTCTGATGTGCTCGGCACGGAA
GATGATGTGATTACGAAAGACTTGGAAGCGGATGTCGATCGGCGCCTGTTGCTGAACGCCTTGCGCCAGCTGAGCGACCG
TGAAAAGCAAATTATGGAACTGCGTTTTGGTCTTTCGGGTGGTGAGGAAAAAACGCAAAAAGATGTTGCTGATTTGCTCG
GTATTTCGCAATCGTACATATCGCGGCTTGAGAAGCGGATCATTAAACGGTTGCGTAAAGAATTCAATAAGATGATGTAG

Upstream 100 bases:

>100_bases
TCGTTCATCCGAAAATGGTGCAGTCGGGAAAAAAGCTGACCGCTTCGTAGTTCGTTACTATATCATACTCGCTTTTTATC
CGTTTAGAAGGGGGATTTTT

Downstream 100 bases:

>100_bases
CCGGCGGCAGGGGCAGATGCTCTGCCGCTTTTTTTCAGCCCGGACAGGCTGTGCATATTTTTCCCTCCTAAGGAGATACT
GAAAACTGACGACTTAGCAT

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKKWKLRFMYWVYKLLAKLGLKTDEIYYIGGSEALPPPLTKEEEERLIERLAVGDKTARSLLIERNLRLVVYIARKFENT
GIHIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKVRAEVSFDEPLNIDWDGNELLLSDVLGTE
DDVITKDLEADVDRRLLLNALRQLSDREKQIMELRFGLSGGEEKTQKDVADLLGISQSYISRLEKRIIKRLRKEFNKMM

Sequences:

>Translated_239_residues
MKKWKLRFMYWVYKLLAKLGLKTDEIYYIGGSEALPPPLTKEEEERLIERLAVGDKTARSLLIERNLRLVVYIARKFENT
GIHIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKVRAEVSFDEPLNIDWDGNELLLSDVLGTE
DDVITKDLEADVDRRLLLNALRQLSDREKQIMELRFGLSGGEEKTQKDVADLLGISQSYISRLEKRIIKRLRKEFNKMM
>Mature_239_residues
MKKWKLRFMYWVYKLLAKLGLKTDEIYYIGGSEALPPPLTKEEEERLIERLAVGDKTARSLLIERNLRLVVYIARKFENT
GIHIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIENEILMYLRRNNKVRAEVSFDEPLNIDWDGNELLLSDVLGTE
DDVITKDLEADVDRRLLLNALRQLSDREKQIMELRFGLSGGEEKTQKDVADLLGISQSYISRLEKRIIKRLRKEFNKMM

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=29.6875, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1789871, Length=106, Percent_Identity=42.4528301886792, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789448, Length=252, Percent_Identity=24.6031746031746, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27806; Mature: 27806

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWKLRFMYWVYKLLAKLGLKTDEIYYIGGSEALPPPLTKEEEERLIERLAVGDKTARS
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
LLIERNLRLVVYIARKFENTGIHIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EILMYLRRNNKVRAEVSFDEPLNIDWDGNELLLSDVLGTEDDVITKDLEADVDRRLLLNA
HHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LRQLSDREKQIMELRFGLSGGEEKTQKDVADLLGISQSYISRLEKRIIKRLRKEFNKMM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKWKLRFMYWVYKLLAKLGLKTDEIYYIGGSEALPPPLTKEEEERLIERLAVGDKTARS
CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
LLIERNLRLVVYIARKFENTGIHIEDLISIGTIGLIKAVNTFNPEKKIKLATYASRCIEN
HHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EILMYLRRNNKVRAEVSFDEPLNIDWDGNELLLSDVLGTEDDVITKDLEADVDRRLLLNA
HHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LRQLSDREKQIMELRFGLSGGEEKTQKDVADLLGISQSYISRLEKRIIKRLRKEFNKMM
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]