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The map label for this gene is pdhC [H]

Identifier: 138894594

GI number: 138894594

Start: 996729

End: 998039

Strand: Direct

Name: pdhC [H]

Synonym: GTNG_0924

Alternate gene names: 138894594

Gene position: 996729-998039 (Clockwise)

Preceding gene: 138894593

Following gene: 138894595

Centisome position: 28.07

GC content: 52.4

Gene sequence:

>1311_bases
GTGGCATTTGAATTTAAGCTGCCGGACATTGGCGAAGGCATTCATGAAGGCGAGATCGTGAAATGGTTTGTGAAACCAGG
CGATGAAGTGAACGAAGATGATGTGTTGTGTGAAGTACAAAACGACAAGGCAGTCGTAGAAATCCCGTCGCCGGTCAAAG
GAAAAGTGCTTGAGATTCTTGTCCCGGAAGGAACCGTTGCGACGGTCGGGCAAACGCTTATCACGCTCGACGCACCGGGA
TATGAAAACATGACGTTTAAAGGACAAGAGCACGAAGAAGAGGCGAAAAAAGAGGAAAAAACGGAAACGGTGTCGAAAGA
AGAAAGCGTTGGGGCCACCGCCCCGGCAGCCGCAGCAGAGGCTGCAGCAGAAGCCGATCCAAATCGTCGCGTCATCGCGA
TGCCGTCTGTGCGCAAATATGCGCGTGAAAAAGGTGTGGACATTCGTCTCGTCCAAGGAACGGGGAAAAATGGCCGCATC
TTGAAAGAAGATATCGATGCTTTCCTTGCTGGCGGTGCGAAAGCTGCAGCCCAACCGGCGCCGGCAGCAGAAGCTGAAGA
AAAAGCAGCGCCGCAGGCAGCGGCGACACCGGTTGTGCCAGAAGGCGAATTCCCGGAAACGCGCGAGAAAATGAGCGGCA
TTCGCCGAGCGATCGCCAAAGCGATGGTGAATTCGAAACATACAGCTCCGCACGTGACGCTCATGGACGAAGTCGACGTG
ACGAAACTAGTTGCCCACCGGAAAAAGTTCAAAGCGATCGCAGCAGAAAAAGGTATCAAGCTGACGTTCTTGCCGTATGT
CGTCAAAGCGCTCGTTTCGGCGTTGCGTGAATATCCGACGCTGAATACGTCAATCGATGATCAAACAGAAGAAATCATCC
ATAAGCACTATTACAACATCGGCATCGCTGCTGACACAGATCGTGGTTTGCTCGTGCCGGTCATTAAGCATGCCGACCGG
AAGCCGATTTTCGCGTTGGCGCAAGAAATTAACGAGCTCGCCGTAAAAGCTCGCGACGGCAAGCTAGCGCCAAATGAAAT
GAAAGGTGCATCGTGCACGATTACAAACATCGGTTCGGCTGGTGGGCAATGGTTCACTCCGGTCATCAATCATCCGGAAG
TAGCGATTCTTGGCATCGGTCGCATCGCGGAAAAACCGATTGTCCGCGACGGCGAAATCGTCGCCGCTCCAGTCTTAGCG
CTCTCGTTGAGCTTCGATCATCGAATGATCGATGGAGCAACGGCACAAAAAGCACTCAACCATGTGAAACGGCTGTTAAG
TGACCCAGAACTATTATTAATGGAGGCGTAA

Upstream 100 bases:

>100_bases
CGGCGAAAAAAGTAATGAACTTCTGATAATGGACAAGTCCCGGGGGAGCAGATCGCATTCCCCCGCCTATTTTCGGCAAT
CGATAGGAGGTCGACATACA

Downstream 100 bases:

>100_bases
AACGATGGTAGTTGGCGATTTTGCAATTGAAACGGAAACGCTCGTCGTCGGCGCCGGCCCTGGCGGCTATGTAGCCGCCA
TCCGCGCTGCACAGCTCGGC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MAFEFKLPDIGEGIHEGEIVKWFVKPGDEVNEDDVLCEVQNDKAVVEIPSPVKGKVLEILVPEGTVATVGQTLITLDAPG
YENMTFKGQEHEEEAKKEEKTETVSKEESVGATAPAAAAEAAAEADPNRRVIAMPSVRKYAREKGVDIRLVQGTGKNGRI
LKEDIDAFLAGGAKAAAQPAPAAEAEEKAAPQAAATPVVPEGEFPETREKMSGIRRAIAKAMVNSKHTAPHVTLMDEVDV
TKLVAHRKKFKAIAAEKGIKLTFLPYVVKALVSALREYPTLNTSIDDQTEEIIHKHYYNIGIAADTDRGLLVPVIKHADR
KPIFALAQEINELAVKARDGKLAPNEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPIVRDGEIVAAPVLA
LSLSFDHRMIDGATAQKALNHVKRLLSDPELLLMEA

Sequences:

>Translated_436_residues
MAFEFKLPDIGEGIHEGEIVKWFVKPGDEVNEDDVLCEVQNDKAVVEIPSPVKGKVLEILVPEGTVATVGQTLITLDAPG
YENMTFKGQEHEEEAKKEEKTETVSKEESVGATAPAAAAEAAAEADPNRRVIAMPSVRKYAREKGVDIRLVQGTGKNGRI
LKEDIDAFLAGGAKAAAQPAPAAEAEEKAAPQAAATPVVPEGEFPETREKMSGIRRAIAKAMVNSKHTAPHVTLMDEVDV
TKLVAHRKKFKAIAAEKGIKLTFLPYVVKALVSALREYPTLNTSIDDQTEEIIHKHYYNIGIAADTDRGLLVPVIKHADR
KPIFALAQEINELAVKARDGKLAPNEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPIVRDGEIVAAPVLA
LSLSFDHRMIDGATAQKALNHVKRLLSDPELLLMEA
>Mature_435_residues
AFEFKLPDIGEGIHEGEIVKWFVKPGDEVNEDDVLCEVQNDKAVVEIPSPVKGKVLEILVPEGTVATVGQTLITLDAPGY
ENMTFKGQEHEEEAKKEEKTETVSKEESVGATAPAAAAEAAAEADPNRRVIAMPSVRKYAREKGVDIRLVQGTGKNGRIL
KEDIDAFLAGGAKAAAQPAPAAEAEEKAAPQAAATPVVPEGEFPETREKMSGIRRAIAKAMVNSKHTAPHVTLMDEVDVT
KLVAHRKKFKAIAAEKGIKLTFLPYVVKALVSALREYPTLNTSIDDQTEEIIHKHYYNIGIAADTDRGLLVPVIKHADRK
PIFALAQEINELAVKARDGKLAPNEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPIVRDGEIVAAPVLAL
SLSFDHRMIDGATAQKALNHVKRLLSDPELLLMEA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=35.9090909090909, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=30.7692307692308, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI19923748, Length=242, Percent_Identity=35.9504132231405, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=28.4444444444444, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=28.4444444444444, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=32.3353293413174, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786305, Length=434, Percent_Identity=35.4838709677419, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786946, Length=438, Percent_Identity=32.8767123287671, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=35.3348729792148, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI25146366, Length=441, Percent_Identity=30.3854875283447, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=29.4642857142857, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=31.4935064935065, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=434, Percent_Identity=31.3364055299539, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=28.3482142857143, Blast_Score=149, Evalue=6e-37,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=37.1559633027523, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=33.9055793991416, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=31.4410480349345, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=31.4410480349345, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47001; Mature: 46870

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFKLPDIGEGIHEGEIVKWFVKPGDEVNEDDVLCEVQNDKAVVEIPSPVKGKVLEIL
CCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCCCEEEEE
VPEGTVATVGQTLITLDAPGYENMTFKGQEHEEEAKKEEKTETVSKEESVGATAPAAAAE
ECCCCHHHCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AAAEADPNRRVIAMPSVRKYAREKGVDIRLVQGTGKNGRILKEDIDAFLAGGAKAAAQPA
HHHCCCCCCEEEECCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCC
PAAEAEEKAAPQAAATPVVPEGEFPETREKMSGIRRAIAKAMVNSKHTAPHVTLMDEVDV
CCCCCHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
TKLVAHRKKFKAIAAEKGIKLTFLPYVVKALVSALREYPTLNTSIDDQTEEIIHKHYYNI
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEE
GIAADTDRGLLVPVIKHADRKPIFALAQEINELAVKARDGKLAPNEMKGASCTITNIGSA
EEEECCCCCEEEHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEECCCC
GGQWFTPVINHPEVAILGIGRIAEKPIVRDGEIVAAPVLALSLSFDHRMIDGATAQKALN
CCCEECCCCCCCCEEEEECCHHHCCCCCCCCCEEEECHHHEEECCCCHHHCCHHHHHHHH
HVKRLLSDPELLLMEA
HHHHHHCCCCEEEECC
>Mature Secondary Structure 
AFEFKLPDIGEGIHEGEIVKWFVKPGDEVNEDDVLCEVQNDKAVVEIPSPVKGKVLEIL
CEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCCCEEEEE
VPEGTVATVGQTLITLDAPGYENMTFKGQEHEEEAKKEEKTETVSKEESVGATAPAAAAE
ECCCCHHHCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AAAEADPNRRVIAMPSVRKYAREKGVDIRLVQGTGKNGRILKEDIDAFLAGGAKAAAQPA
HHHCCCCCCEEEECCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCC
PAAEAEEKAAPQAAATPVVPEGEFPETREKMSGIRRAIAKAMVNSKHTAPHVTLMDEVDV
CCCCCHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHH
TKLVAHRKKFKAIAAEKGIKLTFLPYVVKALVSALREYPTLNTSIDDQTEEIIHKHYYNI
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEE
GIAADTDRGLLVPVIKHADRKPIFALAQEINELAVKARDGKLAPNEMKGASCTITNIGSA
EEEECCCCCEEEHHHHCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEECCCC
GGQWFTPVINHPEVAILGIGRIAEKPIVRDGEIVAAPVLALSLSFDHRMIDGATAQKALN
CCCEECCCCCCCCEEEEECCHHHCCCCCCCCCEEEECHHHEEECCCCHHHCCHHHHHHHH
HVKRLLSDPELLLMEA
HHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 3421911; 1915365; 8445635; 8450544; 8805537 [H]