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The map label for this gene is argF [H]

Identifier: 138894348

GI number: 138894348

Start: 755309

End: 756247

Strand: Direct

Name: argF [H]

Synonym: GTNG_0676

Alternate gene names: 138894348

Gene position: 755309-756247 (Clockwise)

Preceding gene: 138894347

Following gene: 138894349

Centisome position: 21.27

GC content: 51.86

Gene sequence:

>939_bases
ATGAATACAGTGATGTCATTAAAAGGGAGAGACTTTTTAACGCTTCTTGATTTTTCTACAGAAGAGATTTTTGACTTGCT
GGCGTTAGCAGGCGACCTAAAAGCAAAGCAAAAAGCCGGCATGCCGTATACGCCGCTTGCCGGCAAAACGATGGCGATGA
TTTTTGAAAAACCATCGACGCGCACGCGCGTATCGTTTGAAGTCGGCATGATTCAACTTGGCGGCAGTGCGCTTTATTTA
AACAGCAACGACTTGCAGCTCGGTCGCGGTGAAACGATCGCCGATACGGCTCGCGTCCTGTCGCAATACGTCGATGTCAT
TATGATCCGGACATTTGCCCACCAAAAAGTCGAAGAGTTGGCCGAATACGCATCGATTCCGGTCATCAATGGCTTGACGG
ACGACGATCATCCGTGCCAGGCACTGGCGGATTTGCTGACGATTTATGAAGTGAAGAAAACGTTCCAAGGTATCAAGCTC
GCTTATGTCGGGGATGGCAACAATGTTGCCAACGCACTCTTGGCGGCTGCGGCGAAAGTCGGCATGGATGTGGCCATCGC
TTGTCCACCGGGTTATGAGCCGAAGGCAGCTTATGTAGAAGCGGCGCGCCAAATCGGGGAAAAAACAGGCGCGGCGGTCA
CAGTGACGCACGACCCGCTCGAGGCGGTGGCGGGAGCGGATGCGATTTATACGGACGTCTGGACGAGCATGGGACAGGAG
AGTGAGAGCGATGAGCGGCTGCAAGTATTTCAGCCATATCAAGTAAATGAAGCGCTCGTTAAAGCAGCGAAACCGGACTA
TATATTTTTGCATTGCTTACCAGCCCATCGCGGCGAGGAAGTGACGGCCGGTGTCATGGACGGTCCGAATTCGATGGTCT
TTGAACAGGCTGGCAATCGCCTTCACGCACAAAAAGCGATTTTAGTGTCGGTTTTATAA

Upstream 100 bases:

>100_bases
TCGATCTCTTTTTACAGGCGCTCACTGTAGGTCAGCTCGATGTACAGCCGATCAGCGGTTGGATTAAAAAGAAAAAACAG
GCAGAACAGGCGGTGATCGC

Downstream 100 bases:

>100_bases
TGGAAAGAACGATGTCCTGCCAAGTGTGGCGGGACATCTTTTTTATTCGGGGCGGCGCAACTGATGATTTTCGGAGGTAA
TGGAACGATTACAGGCGAAG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNTVMSLKGRDFLTLLDFSTEEIFDLLALAGDLKAKQKAGMPYTPLAGKTMAMIFEKPSTRTRVSFEVGMIQLGGSALYL
NSNDLQLGRGETIADTARVLSQYVDVIMIRTFAHQKVEELAEYASIPVINGLTDDDHPCQALADLLTIYEVKKTFQGIKL
AYVGDGNNVANALLAAAAKVGMDVAIACPPGYEPKAAYVEAARQIGEKTGAAVTVTHDPLEAVAGADAIYTDVWTSMGQE
SESDERLQVFQPYQVNEALVKAAKPDYIFLHCLPAHRGEEVTAGVMDGPNSMVFEQAGNRLHAQKAILVSVL

Sequences:

>Translated_312_residues
MNTVMSLKGRDFLTLLDFSTEEIFDLLALAGDLKAKQKAGMPYTPLAGKTMAMIFEKPSTRTRVSFEVGMIQLGGSALYL
NSNDLQLGRGETIADTARVLSQYVDVIMIRTFAHQKVEELAEYASIPVINGLTDDDHPCQALADLLTIYEVKKTFQGIKL
AYVGDGNNVANALLAAAAKVGMDVAIACPPGYEPKAAYVEAARQIGEKTGAAVTVTHDPLEAVAGADAIYTDVWTSMGQE
SESDERLQVFQPYQVNEALVKAAKPDYIFLHCLPAHRGEEVTAGVMDGPNSMVFEQAGNRLHAQKAILVSVL
>Mature_312_residues
MNTVMSLKGRDFLTLLDFSTEEIFDLLALAGDLKAKQKAGMPYTPLAGKTMAMIFEKPSTRTRVSFEVGMIQLGGSALYL
NSNDLQLGRGETIADTARVLSQYVDVIMIRTFAHQKVEELAEYASIPVINGLTDDDHPCQALADLLTIYEVKKTFQGIKL
AYVGDGNNVANALLAAAAKVGMDVAIACPPGYEPKAAYVEAARQIGEKTGAAVTVTHDPLEAVAGADAIYTDVWTSMGQE
SESDERLQVFQPYQVNEALVKAAKPDYIFLHCLPAHRGEEVTAGVMDGPNSMVFEQAGNRLHAQKAILVSVL

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=46.2783171521036, Blast_Score=282, Evalue=2e-76,
Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=27.3885350318471, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1790703, Length=326, Percent_Identity=44.1717791411043, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1786469, Length=329, Percent_Identity=42.2492401215805, Blast_Score=239, Evalue=1e-64,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=30.8917197452229, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI48994908, Length=363, Percent_Identity=23.1404958677686, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=329, Percent_Identity=26.4437689969605, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=38.8349514563107, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=27.741935483871, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=28.2539682539683, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33688; Mature: 33688

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVMSLKGRDFLTLLDFSTEEIFDLLALAGDLKAKQKAGMPYTPLAGKTMAMIFEKPST
CCCCEECCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCEEEEEEECCCC
RTRVSFEVGMIQLGGSALYLNSNDLQLGRGETIADTARVLSQYVDVIMIRTFAHQKVEEL
CEEEEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEYASIPVINGLTDDDHPCQALADLLTIYEVKKTFQGIKLAYVGDGNNVANALLAAAAKV
HHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHH
GMDVAIACPPGYEPKAAYVEAARQIGEKTGAAVTVTHDPLEAVAGADAIYTDVWTSMGQE
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCHHHHHHHHHCCCC
SESDERLQVFQPYQVNEALVKAAKPDYIFLHCLPAHRGEEVTAGVMDGPNSMVFEQAGNR
CCCCCCEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHCCC
LHAQKAILVSVL
HHHHHEEEEEEC
>Mature Secondary Structure
MNTVMSLKGRDFLTLLDFSTEEIFDLLALAGDLKAKQKAGMPYTPLAGKTMAMIFEKPST
CCCCEECCCCCEEEEECCCHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCEEEEEEECCCC
RTRVSFEVGMIQLGGSALYLNSNDLQLGRGETIADTARVLSQYVDVIMIRTFAHQKVEEL
CEEEEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEYASIPVINGLTDDDHPCQALADLLTIYEVKKTFQGIKLAYVGDGNNVANALLAAAAKV
HHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHH
GMDVAIACPPGYEPKAAYVEAARQIGEKTGAAVTVTHDPLEAVAGADAIYTDVWTSMGQE
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCHHHHHHHHHCCCC
SESDERLQVFQPYQVNEALVKAAKPDYIFLHCLPAHRGEEVTAGVMDGPNSMVFEQAGNR
CCCCCCEEEECCCCHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHCCC
LHAQKAILVSVL
HHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9370352 [H]