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The map label for this gene is yitJ [H]

Identifier: 138894296

GI number: 138894296

Start: 694649

End: 696499

Strand: Reverse

Name: yitJ [H]

Synonym: GTNG_0622

Alternate gene names: 138894296

Gene position: 696499-694649 (Counterclockwise)

Preceding gene: 138894301

Following gene: 138894295

Centisome position: 19.62

GC content: 54.13

Gene sequence:

>1851_bases
GTGGGATTGCTTGATGAGTTAAAACAACGCGTGCTGATCGCTGACGGGGCGATGGGGACGCTTCTATATTCCCATGGTAT
TGACCGTTGTTTTGAAGAATTGAATCTATCAAAACCAGAAGAAATCGTCCATATTCACGAGGCATATATCGCGGCGGGCG
CTGATGTCATCCAGACGAATACATACGGCGCCAACTATGTAAAACTCGCCCGCTACGGCCTTGAGGACGAGGTGCCGTCC
ATCAACCGGGCGGCGGTACGGCTCGCCAAGCAAGCGGCGAACGGGCGGACGCACGTACTCGGAACAATCGGCGGACTGCG
CACATTGAACAAAAGCGCTGTGCCGCTTGAGGAAGTGAAGCGGACGTTCCGCGAGCAGCTGTTTGTCTTGCTTGCTGAGG
GCGTGGACGGCGTGCTGCTTGAGACGTATTACGATTTAGAAGAGTTAGAGACGGTGCTCACCATCGCCCGCAAAGAGACA
ACCTTACCGATCATCGCCCACGTATCGCTTCATGAAGTTGGCGTCTTGCAAGACGGCACGCCGCTCGCTGATGCCCTCGC
ACGCTTAGAAGCGCTTGGCGCCGATGTTGTCGGGCTGAACTGTCGGCTCGGGCCTTACCATATGCTTCAGTCGCTGGAAG
AAGTACCGCTGCCAACGCGCGCCTTTTTATCGGCGTACCCGAACGCGAGCCTCCCCGACTACCGCGACGGTCGGCTCGTC
TATGAGACAAATGCCGAATATTTCGAGGAAACGGCTAAAGCGTTTCGTGACCAAGGGGTGCGCTTAATCGGCGGGTGCTG
CGGCACGACGCCAAAACATATTGAAGCGATGGCTCGGGCGCTTCAAGACCGGAAACCCGTGACGAAAAAAACGGTAAAAC
GGCGTGCCGTGTCAGTATCAGTGCAAGCCGACAACCCGTCGGCAGTAACGCCGCTTTCAGAATTAGCCCGCACGCGCCGC
TCGGTGATCGTTGAGCTCGACCCGCCAAAACAATTAGGTATTGACAAGTTTCTCGTCGGAGCCAAAGCGCTCCATGATGC
CGGCATTGATGCACTAACGCTCGCTGACAACTCGCTCGCCACGCCGCGCATCAGCAACGTCGCGGTCGGCTCAATCGTGA
AAGAACAACTCGGCGTCCGTCCACTCATCCATATTACGTGCCGTGACCGCAACTTGATCGGCTTGCAGTCGCATTTAATG
GGGTTGCATACGCTCGGCATTACCGATGTGCTCGCTATTACCGGCGACCCATCGAAAATCGGTGACTTTCCTGGAGCAAC
ATCGGTGTATGATTTATCATCGTTCGACTTAATCCGCTTAATCAGCCAGTTTAACGAAGGACTGTCGTACTCCGGCAAAC
CGCTCGGGCAAAAAACGAACTTCTCAATCGGCGCAGCGTTCAATCCGAACGTCCGCCATTTAGATAAAGCCGTTGAACGC
ATGGAGAAAAAAATTCAATGCGGCGCTCATTATTTCTTGACCCAACCTGTTTATTCAGAAGAGAAAATCGTTGAGGTGTA
CGAAGCGACGAAGCATCTTCACACACCGATCTACATCGGCATTATGCCGCTTGTGAGCGCGCGCAACGCCGACTTTTTGC
ATCATGAAGTGCCGGGCATTACGCTTTCCGATGAGATTCGCGCCCGCATGGCGGCGTGCGGCAACGACCCAGTCCAGGCA
ACGCGCGAAGGAATTGCCATCGCCAAAGCGCTTATTGACGCTGCATTCGACTTGTTTAACGGCATTTATTTAATTACGCC
GTTTTTGCGTTATGACATGACGGTTGAGCTCATCCGCTACATTCACGAAAAAGAAGCGGCCGCCAAAGAAAGGAAGGTTG
TTCATGGCTAA

Upstream 100 bases:

>100_bases
AGACAGGCGGAAGCCTGGAAGATAAGAAGAAGCGAATGACAAGTCTTCTTCTTAGAAGACTTTTTTCTTTTTATTTACGG
AGAAACGGGGGGATCGGAAC

Downstream 100 bases:

>100_bases
CATCACCTTAGAACAGCAACTACAGCGAAAAATTCTTGTCATCGACGGCGCCATGGGGACGATGATCCAAAGCGCCAACC
TATCAGCATCTGATTTTGGC

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MGLLDELKQRVLIADGAMGTLLYSHGIDRCFEELNLSKPEEIVHIHEAYIAAGADVIQTNTYGANYVKLARYGLEDEVPS
INRAAVRLAKQAANGRTHVLGTIGGLRTLNKSAVPLEEVKRTFREQLFVLLAEGVDGVLLETYYDLEELETVLTIARKET
TLPIIAHVSLHEVGVLQDGTPLADALARLEALGADVVGLNCRLGPYHMLQSLEEVPLPTRAFLSAYPNASLPDYRDGRLV
YETNAEYFEETAKAFRDQGVRLIGGCCGTTPKHIEAMARALQDRKPVTKKTVKRRAVSVSVQADNPSAVTPLSELARTRR
SVIVELDPPKQLGIDKFLVGAKALHDAGIDALTLADNSLATPRISNVAVGSIVKEQLGVRPLIHITCRDRNLIGLQSHLM
GLHTLGITDVLAITGDPSKIGDFPGATSVYDLSSFDLIRLISQFNEGLSYSGKPLGQKTNFSIGAAFNPNVRHLDKAVER
MEKKIQCGAHYFLTQPVYSEEKIVEVYEATKHLHTPIYIGIMPLVSARNADFLHHEVPGITLSDEIRARMAACGNDPVQA
TREGIAIAKALIDAAFDLFNGIYLITPFLRYDMTVELIRYIHEKEAAAKERKVVHG

Sequences:

>Translated_616_residues
MGLLDELKQRVLIADGAMGTLLYSHGIDRCFEELNLSKPEEIVHIHEAYIAAGADVIQTNTYGANYVKLARYGLEDEVPS
INRAAVRLAKQAANGRTHVLGTIGGLRTLNKSAVPLEEVKRTFREQLFVLLAEGVDGVLLETYYDLEELETVLTIARKET
TLPIIAHVSLHEVGVLQDGTPLADALARLEALGADVVGLNCRLGPYHMLQSLEEVPLPTRAFLSAYPNASLPDYRDGRLV
YETNAEYFEETAKAFRDQGVRLIGGCCGTTPKHIEAMARALQDRKPVTKKTVKRRAVSVSVQADNPSAVTPLSELARTRR
SVIVELDPPKQLGIDKFLVGAKALHDAGIDALTLADNSLATPRISNVAVGSIVKEQLGVRPLIHITCRDRNLIGLQSHLM
GLHTLGITDVLAITGDPSKIGDFPGATSVYDLSSFDLIRLISQFNEGLSYSGKPLGQKTNFSIGAAFNPNVRHLDKAVER
MEKKIQCGAHYFLTQPVYSEEKIVEVYEATKHLHTPIYIGIMPLVSARNADFLHHEVPGITLSDEIRARMAACGNDPVQA
TREGIAIAKALIDAAFDLFNGIYLITPFLRYDMTVELIRYIHEKEAAAKERKVVHG
>Mature_615_residues
GLLDELKQRVLIADGAMGTLLYSHGIDRCFEELNLSKPEEIVHIHEAYIAAGADVIQTNTYGANYVKLARYGLEDEVPSI
NRAAVRLAKQAANGRTHVLGTIGGLRTLNKSAVPLEEVKRTFREQLFVLLAEGVDGVLLETYYDLEELETVLTIARKETT
LPIIAHVSLHEVGVLQDGTPLADALARLEALGADVVGLNCRLGPYHMLQSLEEVPLPTRAFLSAYPNASLPDYRDGRLVY
ETNAEYFEETAKAFRDQGVRLIGGCCGTTPKHIEAMARALQDRKPVTKKTVKRRAVSVSVQADNPSAVTPLSELARTRRS
VIVELDPPKQLGIDKFLVGAKALHDAGIDALTLADNSLATPRISNVAVGSIVKEQLGVRPLIHITCRDRNLIGLQSHLMG
LHTLGITDVLAITGDPSKIGDFPGATSVYDLSSFDLIRLISQFNEGLSYSGKPLGQKTNFSIGAAFNPNVRHLDKAVERM
EKKIQCGAHYFLTQPVYSEEKIVEVYEATKHLHTPIYIGIMPLVSARNADFLHHEVPGITLSDEIRARMAACGNDPVQAT
REGIAIAKALIDAAFDLFNGIYLITPFLRYDMTVELIRYIHEKEAAAKERKVVHG

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=325, Percent_Identity=28.9230769230769, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI13162290, Length=302, Percent_Identity=28.1456953642384, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI157266337, Length=312, Percent_Identity=28.525641025641, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1790450, Length=326, Percent_Identity=30.6748466257669, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786456, Length=306, Percent_Identity=28.1045751633987, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535405, Length=339, Percent_Identity=30.6784660766962, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324982, Length=313, Percent_Identity=25.8785942492013, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 67559; Mature: 67427

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLDELKQRVLIADGAMGTLLYSHGIDRCFEELNLSKPEEIVHIHEAYIAAGADVIQTN
CCHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEECC
TYGANYVKLARYGLEDEVPSINRAAVRLAKQAANGRTHVLGTIGGLRTLNKSAVPLEEVK
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCHHHHH
RTFREQLFVLLAEGVDGVLLETYYDLEELETVLTIARKETTLPIIAHVSLHEVGVLQDGT
HHHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCCCC
PLADALARLEALGADVVGLNCRLGPYHMLQSLEEVPLPTRAFLSAYPNASLPDYRDGRLV
CHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEE
YETNAEYFEETAKAFRDQGVRLIGGCCGTTPKHIEAMARALQDRKPVTKKTVKRRAVSVS
EECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHEEEE
VQADNPSAVTPLSELARTRRSVIVELDPPKQLGIDKFLVGAKALHDAGIDALTLADNSLA
EECCCCCCCCHHHHHHHHHHCEEEEECCCHHHCHHHHHHHHHHHHHCCCCEEEECCCCCC
TPRISNVAVGSIVKEQLGVRPLIHITCRDRNLIGLQSHLMGLHTLGITDVLAITGDPSKI
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHCHHHEEEECCCCHHC
GDFPGATSVYDLSSFDLIRLISQFNEGLSYSGKPLGQKTNFSIGAAFNPNVRHLDKAVER
CCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHH
MEKKIQCGAHYFLTQPVYSEEKIVEVYEATKHLHTPIYIGIMPLVSARNADFLHHEVPGI
HHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCHHHHCCCCC
TLSDEIRARMAACGNDPVQATREGIAIAKALIDAAFDLFNGIYLITPFLRYDMTVELIRY
EECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IHEKEAAAKERKVVHG
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GLLDELKQRVLIADGAMGTLLYSHGIDRCFEELNLSKPEEIVHIHEAYIAAGADVIQTN
CHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHEECC
TYGANYVKLARYGLEDEVPSINRAAVRLAKQAANGRTHVLGTIGGLRTLNKSAVPLEEVK
CCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCHHHHH
RTFREQLFVLLAEGVDGVLLETYYDLEELETVLTIARKETTLPIIAHVSLHEVGVLQDGT
HHHHHHHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCCCC
PLADALARLEALGADVVGLNCRLGPYHMLQSLEEVPLPTRAFLSAYPNASLPDYRDGRLV
CHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEE
YETNAEYFEETAKAFRDQGVRLIGGCCGTTPKHIEAMARALQDRKPVTKKTVKRRAVSVS
EECCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHEEEE
VQADNPSAVTPLSELARTRRSVIVELDPPKQLGIDKFLVGAKALHDAGIDALTLADNSLA
EECCCCCCCCHHHHHHHHHHCEEEEECCCHHHCHHHHHHHHHHHHHCCCCEEEECCCCCC
TPRISNVAVGSIVKEQLGVRPLIHITCRDRNLIGLQSHLMGLHTLGITDVLAITGDPSKI
CCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHCHHHEEEECCCCHHC
GDFPGATSVYDLSSFDLIRLISQFNEGLSYSGKPLGQKTNFSIGAAFNPNVRHLDKAVER
CCCCCCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHH
MEKKIQCGAHYFLTQPVYSEEKIVEVYEATKHLHTPIYIGIMPLVSARNADFLHHEVPGI
HHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCHHHHCCCCC
TLSDEIRARMAACGNDPVQATREGIAIAKALIDAAFDLFNGIYLITPFLRYDMTVELIRY
EECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IHEKEAAAKERKVVHG
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]