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The map label for this gene is rlmI [H]

Identifier: 138894205

GI number: 138894205

Start: 593465

End: 594664

Strand: Reverse

Name: rlmI [H]

Synonym: GTNG_0531

Alternate gene names: 138894205

Gene position: 594664-593465 (Counterclockwise)

Preceding gene: 138894215

Following gene: 138894198

Centisome position: 16.75

GC content: 50.08

Gene sequence:

>1200_bases
ATGGGAGTGGAATTGATGGCAAAAGTCATTTTAAAACGCCAACGAAAAAAGCGGCTTGAACAAGGTCATCCATGGATTTT
CCAAAGTGAAGTCGACCGCATTGAAGGGGACATTACGCCAGGTGATTTCGTCGATGTCTATAACCACCAAGGTTATTGGC
TGGCGAAAGGATACATTAACCCGGCCTCACAAATCATTGTGCGCATACTGACGCAAAACCCCGATGACGAACTGAATGAG
GAGTTTTTTATCGAGCGCATCCGCCAGGCATGGGCGTACCGTGAACGGATGATTCCCGGCATCCGCTCTTGCCGCGCCAT
CTATGGAGAGGCCGATTTTCTGCCTGGGCTCATCGTCGATAAATACGAGGATGTGCTCTCTGTACAAATTTTGGCGCTTG
GAATGGAAAAACGTAAAGATTGGATTTTGCGCGGCTTGCTTGATGTGTTCGCACCAAGAGCCATTTATTTGCGCAACGAT
GTGTACGTTCGGGAACTTGAGGGACTTAAACAAGAAAAAGGCTTTTGGTATGGAGAAGCAGAAACAGAAATCGAAATCGA
GGAAAACGGCGTCAAATATATTGTCGATATTGAAGGTGGTCAAAAAACCGGCTTTTTCTTCGACCAGCGGCAAAACCGCG
CCGCCCTCCGCCCGCTCATCGGCCCGGAGACAACCGTGCTTGACTGCTTCACTCATACCGGTTCATTTATGCTAAACGCC
TGTCTGTACGGAGCAAAACACGTCACCGCTGTCGACATTTCCGAACATGCGATTGAAACGGCGAAGCGAAACGCAGCCTT
AAACGGCTTTACAAACGTTGATTTTGTCGTCGCCAACGCCTTTGATTATTTGCGCGAGTCAGTGCGCGCCGGCAAACAGT
GGGATGTCGTCATCATCGACCCACCGGCGTTCGCCAAATCCGCCCATGCGGTTCCGAAAGCGTTGCGCGGCTATAAAGAT
ATCAACTTAAATGGGCTGAAGCTTGTCAAAGACGGCGGCTTTTTCGTCACTTCGAGCTGCTCTTACCATGTCCGTCCGCA
CATGTTTCAAGAGATGGTTGCCGACGCGGCGTTTGACGCCAAAAAAGTGCTGCGCCAAGTATACTGGAGCGGCGCCGGCT
ATGACCACCCGAAGCTACTCGCTGCTGATGAAGGCGACTATTTAAAATTCGCCATTTATGAAGTGCATTCGCGACGTTAA

Upstream 100 bases:

>100_bases
ATCATATCACGGCAGCGGTGATTGTCAACTCTTTTTTCCGTCCGGCGTTGCGCCGCCCACCATGATTCGCTATGATTAAA
ACGCAACGACTGACTATCGA

Downstream 100 bases:

>100_bases
AGAAAAACGCTTAAGGGCTGACCTTAAGCGTTTCTTTTTTGTCCTGAGCGGAGCGGCTGTTCTTTGCGCAGCACACTCAA
ATAAACGAGCATCGAAATAA

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MGVELMAKVILKRQRKKRLEQGHPWIFQSEVDRIEGDITPGDFVDVYNHQGYWLAKGYINPASQIIVRILTQNPDDELNE
EFFIERIRQAWAYRERMIPGIRSCRAIYGEADFLPGLIVDKYEDVLSVQILALGMEKRKDWILRGLLDVFAPRAIYLRND
VYVRELEGLKQEKGFWYGEAETEIEIEENGVKYIVDIEGGQKTGFFFDQRQNRAALRPLIGPETTVLDCFTHTGSFMLNA
CLYGAKHVTAVDISEHAIETAKRNAALNGFTNVDFVVANAFDYLRESVRAGKQWDVVIIDPPAFAKSAHAVPKALRGYKD
INLNGLKLVKDGGFFVTSSCSYHVRPHMFQEMVADAAFDAKKVLRQVYWSGAGYDHPKLLAADEGDYLKFAIYEVHSRR

Sequences:

>Translated_399_residues
MGVELMAKVILKRQRKKRLEQGHPWIFQSEVDRIEGDITPGDFVDVYNHQGYWLAKGYINPASQIIVRILTQNPDDELNE
EFFIERIRQAWAYRERMIPGIRSCRAIYGEADFLPGLIVDKYEDVLSVQILALGMEKRKDWILRGLLDVFAPRAIYLRND
VYVRELEGLKQEKGFWYGEAETEIEIEENGVKYIVDIEGGQKTGFFFDQRQNRAALRPLIGPETTVLDCFTHTGSFMLNA
CLYGAKHVTAVDISEHAIETAKRNAALNGFTNVDFVVANAFDYLRESVRAGKQWDVVIIDPPAFAKSAHAVPKALRGYKD
INLNGLKLVKDGGFFVTSSCSYHVRPHMFQEMVADAAFDAKKVLRQVYWSGAGYDHPKLLAADEGDYLKFAIYEVHSRR
>Mature_398_residues
GVELMAKVILKRQRKKRLEQGHPWIFQSEVDRIEGDITPGDFVDVYNHQGYWLAKGYINPASQIIVRILTQNPDDELNEE
FFIERIRQAWAYRERMIPGIRSCRAIYGEADFLPGLIVDKYEDVLSVQILALGMEKRKDWILRGLLDVFAPRAIYLRNDV
YVRELEGLKQEKGFWYGEAETEIEIEENGVKYIVDIEGGQKTGFFFDQRQNRAALRPLIGPETTVLDCFTHTGSFMLNAC
LYGAKHVTAVDISEHAIETAKRNAALNGFTNVDFVVANAFDYLRESVRAGKQWDVVIIDPPAFAKSAHAVPKALRGYKDI
NLNGLKLVKDGGFFVTSSCSYHVRPHMFQEMVADAAFDAKKVLRQVYWSGAGYDHPKLLAADEGDYLKFAIYEVHSRR

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=390, Percent_Identity=36.6666666666667, Blast_Score=274, Evalue=6e-75,
Organism=Escherichia coli, GI1787181, Length=279, Percent_Identity=29.3906810035842, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45555; Mature: 45423

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVELMAKVILKRQRKKRLEQGHPWIFQSEVDRIEGDITPGDFVDVYNHQGYWLAKGYIN
CCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCCCEEEECCCCEEEEECCCC
PASQIIVRILTQNPDDELNEEFFIERIRQAWAYRERMIPGIRSCRAIYGEADFLPGLIVD
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHH
KYEDVLSVQILALGMEKRKDWILRGLLDVFAPRAIYLRNDVYVRELEGLKQEKGFWYGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCHHHHCCEEECCC
ETEIEIEENGVKYIVDIEGGQKTGFFFDQRQNRAALRPLIGPETTVLDCFTHTGSFMLNA
CCEEEEECCCEEEEEEECCCCCCCCEECCCCCCHHHCCCCCCCCCEEHHHHHHHHHHHHH
CLYGAKHVTAVDISEHAIETAKRNAALNGFTNVDFVVANAFDYLRESVRAGKQWDVVIID
HHHCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEC
PPAFAKSAHAVPKALRGYKDINLNGLKLVKDGGFFVTSSCSYHVRPHMFQEMVADAAFDA
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHH
KKVLRQVYWSGAGYDHPKLLAADEGDYLKFAIYEVHSRR
HHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEEEECCCC
>Mature Secondary Structure 
GVELMAKVILKRQRKKRLEQGHPWIFQSEVDRIEGDITPGDFVDVYNHQGYWLAKGYIN
CHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCCCEEEECCCCEEEEECCCC
PASQIIVRILTQNPDDELNEEFFIERIRQAWAYRERMIPGIRSCRAIYGEADFLPGLIVD
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHH
KYEDVLSVQILALGMEKRKDWILRGLLDVFAPRAIYLRNDVYVRELEGLKQEKGFWYGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCHHHHCCEEECCC
ETEIEIEENGVKYIVDIEGGQKTGFFFDQRQNRAALRPLIGPETTVLDCFTHTGSFMLNA
CCEEEEECCCEEEEEEECCCCCCCCEECCCCCCHHHCCCCCCCCCEEHHHHHHHHHHHHH
CLYGAKHVTAVDISEHAIETAKRNAALNGFTNVDFVVANAFDYLRESVRAGKQWDVVIID
HHHCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEC
PPAFAKSAHAVPKALRGYKDINLNGLKLVKDGGFFVTSSCSYHVRPHMFQEMVADAAFDA
CCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHH
KKVLRQVYWSGAGYDHPKLLAADEGDYLKFAIYEVHSRR
HHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA