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The map label for this gene is fumA [H]

Identifier: 138894072

GI number: 138894072

Start: 448224

End: 449771

Strand: Direct

Name: fumA [H]

Synonym: GTNG_0398

Alternate gene names: 138894072

Gene position: 448224-449771 (Clockwise)

Preceding gene: 138894070

Following gene: 138894073

Centisome position: 12.62

GC content: 50.39

Gene sequence:

>1548_bases
ATGGACAAATTCCAGCAAAGCATGTATGACTTAATCGTCGAGACGTCGACGAAGTTGCCTAAAGACGTTCGTCGAGCAAT
CGCTCAGGCAAAAGTGCGTGAAAATCTGGGGACACGTGCAGCTATGGCGCTCGATACAATCGTTGAGAACATTCAAATGG
CTGATGAAAACGTTTCACCGATCTGTCAAGACACCGGTTTGCCCACATTTAAAATCAAAGTTCCAGTCGGCGTGAATCAA
ATTGAAATGAAAAAAGCGATCCGTGCCGCCGTTGTCGAAGCGACGAAAAACGGCAAGCTGCGTCCGAACTCGGTCGATTC
GCTCACTGGCAAAAACAGCGGCGACAACTTAGGAGAAGGCGTACCAGTCATTAAATTTGAACAATGGGAAAACGATTACA
TTGACGTCCGCTTAATTTTGAAAGGCGGCGGCTGTGAAAACAAAAACATTCAATACAGCCTTCCGTGTGAACTCGAAGGC
CTCGGCCGCGCCGGACGCGACCTTGACGGCATTCGCAAATGCATTTTGCATGCGGTGTACCAAGCCCAAGGGCAAGGATG
CAGCGCTGGTTTTATCGGTGTCGGCATTGGCGGCGACCGCTCATCCGGCTATGAGCTTGCCAAAGAGCAGCTGTTCCGTC
CGGTCGATGATGTCAACCCGATTGAAGAGCTGCGCCAACTTGAAGAATACATTATGGAAAACGCCAATAAGCTCGGCATC
GGTACGATGGGATTTGGCGGTGAATCGACATTGCTTGGCTGTAAAATCGGCGTTATGCACCGCATCCCGGCGAGCTTCTT
CGTGTCCGTTGCCTACAACTGTTGGGCGTTCCGCCGTATGGGCGTGAAAATCGACCCGCAAACCGGCGAAATCATGGAAT
GGCTATACCAAGATGGCGAGGATATTGATTTGACCAAAGAACTTGAAAAAGCAGAAGCCGCTGCGGCGCTTGAACAAGGG
GAGACGCGCGTCATCGATCTTGTTCCGCCGCTATCTGAAGAACAAGTGCGCCAGCTTCGTGTCGGCGATGTTGTCCGCAT
CAGCGGCATGATTTATACGGGACGCGACGCCATTCATAAATATTTAATGGACCATGATGCTCCGGTCGATTTGAATGGGC
AAATCATTTACCATTGCGGTCCGGTCATGTTAAAAGATGAAGAAGGCCGTTGGCATGTCAAAGCGGCAGGTCCAACGACA
AGCATCCGTGAGGAGCCGTACCAAGGTGACATTATGAAAAAATTCGGCGTTCGCGCTGTCATTGGCAAAGGCGGCATGGG
TGCGAAAACATTGCAGGCCTTAAAAGAGCATGGCGGTGTCTACTTAAATGCCATCGGCGGTGCAGCTCAATATTACGCTG
ACTGTATCCAATCGGTAGAAGGCGTCGATTTGATGGAATTCGGCATTCCAGAAGCCATGTGGCATTTGCGCGTTGAAAAT
TTCACTGCTGTCGTCACGATGGACTCGCACGGCAACAGCTTGCACGAGGATGTTGAAAAATCATCGTTGGAAAAGCTGGC
CCAATTCAAAGAGCCGGTGTTCAAATAA

Upstream 100 bases:

>100_bases
GTTGTGGAAAATAAAGATTTTTTACACAACAAGGAAAAAGGGAGTATAATAAAGGAAGATTGGACAATTGGATGAACAGG
GGAAGGGGCGTTGAACGGAA

Downstream 100 bases:

>100_bases
ACGTGAAGAAGCAACTGCAACCGAATGGCTAGTGAAAAGATGGCTTGGCCCCAAAAGCGTGTTTTGTATGCGCTTTTGGG
GCTTTTTTGTAAAAAATGGC

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MDKFQQSMYDLIVETSTKLPKDVRRAIAQAKVRENLGTRAAMALDTIVENIQMADENVSPICQDTGLPTFKIKVPVGVNQ
IEMKKAIRAAVVEATKNGKLRPNSVDSLTGKNSGDNLGEGVPVIKFEQWENDYIDVRLILKGGGCENKNIQYSLPCELEG
LGRAGRDLDGIRKCILHAVYQAQGQGCSAGFIGVGIGGDRSSGYELAKEQLFRPVDDVNPIEELRQLEEYIMENANKLGI
GTMGFGGESTLLGCKIGVMHRIPASFFVSVAYNCWAFRRMGVKIDPQTGEIMEWLYQDGEDIDLTKELEKAEAAAALEQG
ETRVIDLVPPLSEEQVRQLRVGDVVRISGMIYTGRDAIHKYLMDHDAPVDLNGQIIYHCGPVMLKDEEGRWHVKAAGPTT
SIREEPYQGDIMKKFGVRAVIGKGGMGAKTLQALKEHGGVYLNAIGGAAQYYADCIQSVEGVDLMEFGIPEAMWHLRVEN
FTAVVTMDSHGNSLHEDVEKSSLEKLAQFKEPVFK

Sequences:

>Translated_515_residues
MDKFQQSMYDLIVETSTKLPKDVRRAIAQAKVRENLGTRAAMALDTIVENIQMADENVSPICQDTGLPTFKIKVPVGVNQ
IEMKKAIRAAVVEATKNGKLRPNSVDSLTGKNSGDNLGEGVPVIKFEQWENDYIDVRLILKGGGCENKNIQYSLPCELEG
LGRAGRDLDGIRKCILHAVYQAQGQGCSAGFIGVGIGGDRSSGYELAKEQLFRPVDDVNPIEELRQLEEYIMENANKLGI
GTMGFGGESTLLGCKIGVMHRIPASFFVSVAYNCWAFRRMGVKIDPQTGEIMEWLYQDGEDIDLTKELEKAEAAAALEQG
ETRVIDLVPPLSEEQVRQLRVGDVVRISGMIYTGRDAIHKYLMDHDAPVDLNGQIIYHCGPVMLKDEEGRWHVKAAGPTT
SIREEPYQGDIMKKFGVRAVIGKGGMGAKTLQALKEHGGVYLNAIGGAAQYYADCIQSVEGVDLMEFGIPEAMWHLRVEN
FTAVVTMDSHGNSLHEDVEKSSLEKLAQFKEPVFK
>Mature_515_residues
MDKFQQSMYDLIVETSTKLPKDVRRAIAQAKVRENLGTRAAMALDTIVENIQMADENVSPICQDTGLPTFKIKVPVGVNQ
IEMKKAIRAAVVEATKNGKLRPNSVDSLTGKNSGDNLGEGVPVIKFEQWENDYIDVRLILKGGGCENKNIQYSLPCELEG
LGRAGRDLDGIRKCILHAVYQAQGQGCSAGFIGVGIGGDRSSGYELAKEQLFRPVDDVNPIEELRQLEEYIMENANKLGI
GTMGFGGESTLLGCKIGVMHRIPASFFVSVAYNCWAFRRMGVKIDPQTGEIMEWLYQDGEDIDLTKELEKAEAAAALEQG
ETRVIDLVPPLSEEQVRQLRVGDVVRISGMIYTGRDAIHKYLMDHDAPVDLNGQIIYHCGPVMLKDEEGRWHVKAAGPTT
SIREEPYQGDIMKKFGVRAVIGKGGMGAKTLQALKEHGGVYLNAIGGAAQYYADCIQSVEGVDLMEFGIPEAMWHLRVEN
FTAVVTMDSHGNSLHEDVEKSSLEKLAQFKEPVFK

Specific function: Unknown

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1789443, Length=171, Percent_Identity=35.672514619883, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787897, Length=476, Percent_Identity=27.3109243697479, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789442, Length=281, Percent_Identity=28.8256227758007, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790564, Length=474, Percent_Identity=26.7932489451477, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 56729; Mature: 56729

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFQQSMYDLIVETSTKLPKDVRRAIAQAKVRENLGTRAAMALDTIVENIQMADENVSP
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
ICQDTGLPTFKIKVPVGVNQIEMKKAIRAAVVEATKNGKLRPNSVDSLTGKNSGDNLGEG
HHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VPVIKFEQWENDYIDVRLILKGGGCENKNIQYSLPCELEGLGRAGRDLDGIRKCILHAVY
CCEEEEECCCCCEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
QAQGQGCSAGFIGVGIGGDRSSGYELAKEQLFRPVDDVNPIEELRQLEEYIMENANKLGI
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCE
GTMGFGGESTLLGCKIGVMHRIPASFFVSVAYNCWAFRRMGVKIDPQTGEIMEWLYQDGE
EECCCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCC
DIDLTKELEKAEAAAALEQGETRVIDLVPPLSEEQVRQLRVGDVVRISGMIYTGRDAIHK
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCEEEEEEEEEECHHHHHH
YLMDHDAPVDLNGQIIYHCGPVMLKDEEGRWHVKAAGPTTSIREEPYQGDIMKKFGVRAV
HHHCCCCCCCCCCEEEEEECCEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCEEE
IGKGGMGAKTLQALKEHGGVYLNAIGGAAQYYADCIQSVEGVDLMEFGIPEAMWHLRVEN
ECCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHCCCHHHHHEEECC
FTAVVTMDSHGNSLHEDVEKSSLEKLAQFKEPVFK
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDKFQQSMYDLIVETSTKLPKDVRRAIAQAKVRENLGTRAAMALDTIVENIQMADENVSP
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
ICQDTGLPTFKIKVPVGVNQIEMKKAIRAAVVEATKNGKLRPNSVDSLTGKNSGDNLGEG
HHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VPVIKFEQWENDYIDVRLILKGGGCENKNIQYSLPCELEGLGRAGRDLDGIRKCILHAVY
CCEEEEECCCCCEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHH
QAQGQGCSAGFIGVGIGGDRSSGYELAKEQLFRPVDDVNPIEELRQLEEYIMENANKLGI
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCE
GTMGFGGESTLLGCKIGVMHRIPASFFVSVAYNCWAFRRMGVKIDPQTGEIMEWLYQDGE
EECCCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHCCCC
DIDLTKELEKAEAAAALEQGETRVIDLVPPLSEEQVRQLRVGDVVRISGMIYTGRDAIHK
CCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCEEEEEEEEEECHHHHHH
YLMDHDAPVDLNGQIIYHCGPVMLKDEEGRWHVKAAGPTTSIREEPYQGDIMKKFGVRAV
HHHCCCCCCCCCCEEEEEECCEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCEEE
IGKGGMGAKTLQALKEHGGVYLNAIGGAAQYYADCIQSVEGVDLMEFGIPEAMWHLRVEN
ECCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHCCCHHHHHEEECC
FTAVVTMDSHGNSLHEDVEKSSLEKLAQFKEPVFK
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8473853 [H]