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The map label for this gene is purN [H]

Identifier: 138893922

GI number: 138893922

Start: 274519

End: 275148

Strand: Direct

Name: purN [H]

Synonym: GTNG_0246

Alternate gene names: 138893922

Gene position: 274519-275148 (Clockwise)

Preceding gene: 138893921

Following gene: 138893923

Centisome position: 7.73

GC content: 53.33

Gene sequence:

>630_bases
ATGAAGCGGTTGGCCGTGTTTGCTTCTGGAAGCGGAACGAATTTTCAGGCGATCGTCGATGCGGTGAAGCGCGGTGAGTT
GCCGGCCGATCTTGCCCTTCTTGTGTGCGACCGTCCAGGTGCGAAGGTGATCGAACGAGCAGCACGCGAAAATGTGCCGA
CGTTCGTGTTTTCTCCAAAAGACTATCCGTCCAAAGCGGCGTTTGAGAGCGAGATTTTGCGTGAATTAAGCGAACGTCAA
ATTGAGTGGATCGCGTTAGCTGGCTATATGCGCTTAATTGGACCGACATTGCTTTCCGCTTATGAAGGGAAAATCGTCAA
CATTCATCCGTCGCTATTGCCGGCGTTTCCGGGTAAAGACGCCATTGGCCAAGCGTATCGGGCTGGCGTTTTGGAAACAG
GCGTCACCATCCATTATGTTGATGAAGGGATGGACACCGGGCCGGTCATCGCCCAGCGCGCTGTCCCGATTGTACCCGGC
GAACCGATCGAAGCGCTTGAGGCACGCATCCATGCGGTTGAACATGAGTTATACCCGGCGGTGTTGCGCATGCTGTTAGG
GGAAACGGAACAACAAGAAGAAAGGATCGAGAAGAATGGCAGTCAAACGAGCATTAATCAGCGTGTCTAA

Upstream 100 bases:

>100_bases
CGCCGCTTGTGCAATGGTTGGACGAACAAAACGAGCCGGCCTACATCATTGGAGAAGTGGTGGAAGGAGCAGGCGTGTCG
TTTACCGGAGGGAACGAAGC

Downstream 100 bases:

>100_bases
TAAAGAAGGGATCATTCCGTTTGCAAAACAACTAACCGAACTCGGCATCGAAATCATTTCGACTGGCGGGACGAAACGGG
CACTCGAGGACGCTGGGATT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKRLAVFASGSGTNFQAIVDAVKRGELPADLALLVCDRPGAKVIERAARENVPTFVFSPKDYPSKAAFESEILRELSERQ
IEWIALAGYMRLIGPTLLSAYEGKIVNIHPSLLPAFPGKDAIGQAYRAGVLETGVTIHYVDEGMDTGPVIAQRAVPIVPG
EPIEALEARIHAVEHELYPAVLRMLLGETEQQEERIEKNGSQTSINQRV

Sequences:

>Translated_209_residues
MKRLAVFASGSGTNFQAIVDAVKRGELPADLALLVCDRPGAKVIERAARENVPTFVFSPKDYPSKAAFESEILRELSERQ
IEWIALAGYMRLIGPTLLSAYEGKIVNIHPSLLPAFPGKDAIGQAYRAGVLETGVTIHYVDEGMDTGPVIAQRAVPIVPG
EPIEALEARIHAVEHELYPAVLRMLLGETEQQEERIEKNGSQTSINQRV
>Mature_209_residues
MKRLAVFASGSGTNFQAIVDAVKRGELPADLALLVCDRPGAKVIERAARENVPTFVFSPKDYPSKAAFESEILRELSERQ
IEWIALAGYMRLIGPTLLSAYEGKIVNIHPSLLPAFPGKDAIGQAYRAGVLETGVTIHYVDEGMDTGPVIAQRAVPIVPG
EPIEALEARIHAVEHELYPAVLRMLLGETEQQEERIEKNGSQTSINQRV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=36.2694300518135, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=36.2694300518135, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=36.2694300518135, Blast_Score=146, Evalue=1e-35,
Organism=Escherichia coli, GI1788846, Length=183, Percent_Identity=33.879781420765, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1787483, Length=78, Percent_Identity=42.3076923076923, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=180, Percent_Identity=36.1111111111111, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=32.8282828282828, Blast_Score=96, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24582400, Length=190, Percent_Identity=41.0526315789474, Blast_Score=166, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22872; Mature: 22872

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLAVFASGSGTNFQAIVDAVKRGELPADLALLVCDRPGAKVIERAARENVPTFVFSPK
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCC
DYPSKAAFESEILRELSERQIEWIALAGYMRLIGPTLLSAYEGKIVNIHPSLLPAFPGKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCH
AIGQAYRAGVLETGVTIHYVDEGMDTGPVIAQRAVPIVPGEPIEALEARIHAVEHELYPA
HHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLRMLLGETEQQEERIEKNGSQTSINQRV
HHHHHHCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKRLAVFASGSGTNFQAIVDAVKRGELPADLALLVCDRPGAKVIERAARENVPTFVFSPK
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCC
DYPSKAAFESEILRELSERQIEWIALAGYMRLIGPTLLSAYEGKIVNIHPSLLPAFPGKD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCH
AIGQAYRAGVLETGVTIHYVDEGMDTGPVIAQRAVPIVPGEPIEALEARIHAVEHELYPA
HHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLRMLLGETEQQEERIEKNGSQTSINQRV
HHHHHHCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]