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The map label for this gene is purT

Identifier: 126696431

GI number: 126696431

Start: 933306

End: 934481

Strand: Reverse

Name: purT

Synonym: P9301_10931

Alternate gene names: 126696431

Gene position: 934481-933306 (Counterclockwise)

Preceding gene: 126696432

Following gene: 126696430

Centisome position: 56.92

GC content: 31.46

Gene sequence:

>1176_bases
ATGAAAGAATCAATTTTTTCTAAAAAGAGAATTTTATTGCTTGGTAGTGGCGAGCTTGGAAAAGAATTAGTAATAGAATC
CAAAAGATTAGGATTAGAAGTTATTGCAATTGATCGATATGAAAAAGCACCTGCCATGCAAGTTGCTGATTATTCAAGAG
TAATTGATATGGGAGATAAAAATATTTTAAAAAATGTTATAAAAGAATTCAATCCTGACTATGTTGTCCCAGAAATAGAG
GCACTTTCAATTGAAGCCCTTAAAGAACTCGAGGATGAAGGATTCAATATTGTTCCCAATGCCAGAACTGTAGAAATCAC
AATGAATAGAGATAAAATTAGAGACTTAGCTTCTAAAGATTTAAAAATTAAAACTGCAAAGTTTGATTATATTTTTGAAT
TTGATGATTTAGAAAAAAAAGCAGATGAAATTGGATTCCCACTTTTACTTAAGCCTTTAATGAGCTCTTCAGGAAAAGGG
CAGAGTTTGGTTGAAACAAAAAATGATTTACAAAATGCTTGGAAACAGGCACAAGCAAATTCAAGAGGAAAGGTTAAAGG
TGTAATTATTGAAGAATTTATTAACTTTGATTTTGAGTTTACTCTTCTAACTGTAAGAAAAGAAAATGGTGAAAATATTT
TTTGTTTACCAATTGGGCATCTTCAATCTAATGGTGACTATCAATGTAGTTGGCAACCTTTAGAGATTAAGGATTCCTTA
ATTATTGAAGCTAAGAGAATGACAAGCAGAATATTAAATAACCTTAATGGAGCTGGATTATACGGAGTAGAGTTTTTTAT
AAAAGGAAGTGAGGTTATATTCTCAGAATTATCTCCAAGACCACACGACACTGGTATGGTTACATTAGTTAGTCAAAATA
TTAATGAATTTGAATTACACTTAAGGGCTTTTTTGGATTTACCAATACCGCATATTTATCTAATAGAACCCTCTGCAACC
AGAGTTATACTTTCTAACCAAGAGTATCTAAATCCTATTTATGAGGGTCTTAACGAAGCATTAGAATTTGAAAAGACTAA
AGTGCTAATATTTGGCAAACCAGTTTCCAGAAAAGGCAGAAGAATGGGTGTTGTTCTCTCATCAAATTCTGACATTTATT
TGGCTAGAAAAAATGCAGATGAAGCTGCTCGTAAGATAAAAGTGAGTACTACATAA

Upstream 100 bases:

>100_bases
CGTTTGGTAGAAATTTTTTATTTAGTTATTCTAAAGATAAAAAAATTACAGATGATACATCTTCACAAACTTTTGGAGAT
GACGAATAAGTTTTATTTAC

Downstream 100 bases:

>100_bases
ATATTAAATAAAAATAACGAAAAAAATACTTATTTCCTTTGTTTTTGTTCTATGTCTCTCTGGGTATTATTTGAATAAGT
TATCTCTTTCACAATGATAG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MKESIFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSRVIDMGDKNILKNVIKEFNPDYVVPEIE
ALSIEALKELEDEGFNIVPNARTVEITMNRDKIRDLASKDLKIKTAKFDYIFEFDDLEKKADEIGFPLLLKPLMSSSGKG
QSLVETKNDLQNAWKQAQANSRGKVKGVIIEEFINFDFEFTLLTVRKENGENIFCLPIGHLQSNGDYQCSWQPLEIKDSL
IIEAKRMTSRILNNLNGAGLYGVEFFIKGSEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFLDLPIPHIYLIEPSAT
RVILSNQEYLNPIYEGLNEALEFEKTKVLIFGKPVSRKGRRMGVVLSSNSDIYLARKNADEAARKIKVSTT

Sequences:

>Translated_391_residues
MKESIFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSRVIDMGDKNILKNVIKEFNPDYVVPEIE
ALSIEALKELEDEGFNIVPNARTVEITMNRDKIRDLASKDLKIKTAKFDYIFEFDDLEKKADEIGFPLLLKPLMSSSGKG
QSLVETKNDLQNAWKQAQANSRGKVKGVIIEEFINFDFEFTLLTVRKENGENIFCLPIGHLQSNGDYQCSWQPLEIKDSL
IIEAKRMTSRILNNLNGAGLYGVEFFIKGSEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFLDLPIPHIYLIEPSAT
RVILSNQEYLNPIYEGLNEALEFEKTKVLIFGKPVSRKGRRMGVVLSSNSDIYLARKNADEAARKIKVSTT
>Mature_391_residues
MKESIFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSRVIDMGDKNILKNVIKEFNPDYVVPEIE
ALSIEALKELEDEGFNIVPNARTVEITMNRDKIRDLASKDLKIKTAKFDYIFEFDDLEKKADEIGFPLLLKPLMSSSGKG
QSLVETKNDLQNAWKQAQANSRGKVKGVIIEEFINFDFEFTLLTVRKENGENIFCLPIGHLQSNGDYQCSWQPLEIKDSL
IIEAKRMTSRILNNLNGAGLYGVEFFIKGSEVIFSELSPRPHDTGMVTLVSQNINEFELHLRAFLDLPIPHIYLIEPSAT
RVILSNQEYLNPIYEGLNEALEFEKTKVLIFGKPVSRKGRRMGVVLSSNSDIYLARKNADEAARKIKVSTT

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=382, Percent_Identity=45.2879581151832, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1786733, Length=367, Percent_Identity=25.3405994550409, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=371, Percent_Identity=25.0673854447439, Blast_Score=105, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_PROM0 (A3PD91)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091317.1
- ProteinModelPortal:   A3PD91
- SMR:   A3PD91
- STRING:   A3PD91
- GeneID:   4912637
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_10931
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- BioCyc:   PMAR167546:P9301ORF_1115-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 44267; Mature: 44267

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 78-78 BINDING 110-110 BINDING 151-151 BINDING 199-199 BINDING 264-264 BINDING 276-276 BINDING 283-283 BINDING 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESIFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSRVIDMGDK
CCCCCCCCCEEEEEECCCCCHHHEEEHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCH
NILKNVIKEFNPDYVVPEIEALSIEALKELEDEGFNIVPNARTVEITMNRDKIRDLASKD
HHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHCCC
LKIKTAKFDYIFEFDDLEKKADEIGFPLLLKPLMSSSGKGQSLVETKNDLQNAWKQAQAN
CEEEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
SRGKVKGVIIEEFINFDFEFTLLTVRKENGENIFCLPIGHLQSNGDYQCSWQPLEIKDSL
CCCCEEEEEEHHHHCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCEECCHH
IIEAKRMTSRILNNLNGAGLYGVEFFIKGSEVIFSELSPRPHDTGMVTLVSQNINEFELH
EEEHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHCCCCCCCCCEEEEEECCCCHHHHH
LRAFLDLPIPHIYLIEPSATRVILSNQEYLNPIYEGLNEALEFEKTKVLIFGKPVSRKGR
HHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
RMGVVLSSNSDIYLARKNADEAARKIKVSTT
EEEEEEECCCCEEEEECCCHHHHHEEEEECC
>Mature Secondary Structure
MKESIFSKKRILLLGSGELGKELVIESKRLGLEVIAIDRYEKAPAMQVADYSRVIDMGDK
CCCCCCCCCEEEEEECCCCCHHHEEEHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCH
NILKNVIKEFNPDYVVPEIEALSIEALKELEDEGFNIVPNARTVEITMNRDKIRDLASKD
HHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEECCCCEEEEEEECHHHHHHHHCCC
LKIKTAKFDYIFEFDDLEKKADEIGFPLLLKPLMSSSGKGQSLVETKNDLQNAWKQAQAN
CEEEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
SRGKVKGVIIEEFINFDFEFTLLTVRKENGENIFCLPIGHLQSNGDYQCSWQPLEIKDSL
CCCCEEEEEEHHHHCCCEEEEEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCEECCHH
IIEAKRMTSRILNNLNGAGLYGVEFFIKGSEVIFSELSPRPHDTGMVTLVSQNINEFELH
EEEHHHHHHHHHHCCCCCCEEEEEEEEECCHHHHHHCCCCCCCCCEEEEEECCCCHHHHH
LRAFLDLPIPHIYLIEPSATRVILSNQEYLNPIYEGLNEALEFEKTKVLIFGKPVSRKGR
HHHHHCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
RMGVVLSSNSDIYLARKNADEAARKIKVSTT
EEEEEEECCCCEEEEECCCHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA