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The map label for this gene is selU [H]

Identifier: 126696272

GI number: 126696272

Start: 803220

End: 804260

Strand: Reverse

Name: selU [H]

Synonym: P9301_09341

Alternate gene names: 126696272

Gene position: 804260-803220 (Counterclockwise)

Preceding gene: 126696275

Following gene: 126696271

Centisome position: 48.98

GC content: 26.99

Gene sequence:

>1041_bases
ATGTATTTCAAAAGAAAAGAACTAGAGAAATTTAGATGTTTTAAAGGACCACTTATAGATGTTAGGAGCCCGAGTGAATA
TTATAAAGGACACATGCCTAATTCTATTAATATTCCTCTATTTGATGATGATGAGAGATCTATAATTGGTACAATTTACA
AAAAAGAAGGTAGAAGAAAAGCAGTCATAGAGGGATTAAAATTTTTTGAAAATAAAATGGAATTACTTCTTGATAATTTA
TTCAAGAGTATTGAGTCTCATACTACTATTCCTAATAAAAATAATGAATTATTTATCAGGATATATTGCTCTAGAGGAGG
AATGCGTTCACAAAGTATTGGATGGTTATTAGATAAATTTAAATTAAATATAGTTACACTTAATGGCGGATACAAAATAT
ATAGAAGATGGGTATTAGATAGTTTTTCAAATAAGTTGAATTTAGTAGTTATTGGCGGGAAAACAGGAACAGGGAAGACA
AGATTATTATCATTACTTGATAAATATAAATATCAAACTATTGATCTTGAAGGATTTGCTTGTCATAGAGGAAGTACATT
TGGAGGTTTAGGAATGAAAAAACAACCTTCAAATGAACAATTTGAAAATATAATTGCAGAAAAATTAAATTCTTTTAAAT
TTTCTAATAATATTTTTGTAGAAGCTGAAAGTGCAAATATAGGTAAATGTAAAATTCCTCATGAATTCTTCAATCAGATG
AAAAACTCTAGGAGGATTGAAATTATAAGGAGTGAATCTAACAGGTTAGATGAGTTAATAGATACTTATAGTGTATTTAA
AAAAGAGGAACTCCAAGAATCAGTACTAAGGATAAAAAAAAGACTAGGACCGCAAAGAACAAAAATAGCTCTTGAATCAA
TTCATAATGAGAAATGGGACTTAGTTTGCAAATCAGTTTTAGATTATTACGATAAGTGTTATGAATATGAAAAGGTTGGC
AAAACTAATATAGAGATAATAGATTTAACTGATAAAAAATATGATAAAAGTATCCTAGAGTTAATAAATAATGTTTTATA
A

Upstream 100 bases:

>100_bases
CTACAAATTTTTTTATAAGTTCTAATGTAGCATTACTATAGTTATCCTGTTCTTTGTTATTCATTTTAAAAAATTCAATC
TAAAAAAATAAAAACTATCC

Downstream 100 bases:

>100_bases
AATAACTACAAACGAATATGAAAAATATTTCCTAATATAAAAAATTATTGACCAAATATTATGACGACAAATAAAACTGC
AAAAATACAATTTTATGAGG

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MYFKRKELEKFRCFKGPLIDVRSPSEYYKGHMPNSINIPLFDDDERSIIGTIYKKEGRRKAVIEGLKFFENKMELLLDNL
FKSIESHTTIPNKNNELFIRIYCSRGGMRSQSIGWLLDKFKLNIVTLNGGYKIYRRWVLDSFSNKLNLVVIGGKTGTGKT
RLLSLLDKYKYQTIDLEGFACHRGSTFGGLGMKKQPSNEQFENIIAEKLNSFKFSNNIFVEAESANIGKCKIPHEFFNQM
KNSRRIEIIRSESNRLDELIDTYSVFKKEELQESVLRIKKRLGPQRTKIALESIHNEKWDLVCKSVLDYYDKCYEYEKVG
KTNIEIIDLTDKKYDKSILELINNVL

Sequences:

>Translated_346_residues
MYFKRKELEKFRCFKGPLIDVRSPSEYYKGHMPNSINIPLFDDDERSIIGTIYKKEGRRKAVIEGLKFFENKMELLLDNL
FKSIESHTTIPNKNNELFIRIYCSRGGMRSQSIGWLLDKFKLNIVTLNGGYKIYRRWVLDSFSNKLNLVVIGGKTGTGKT
RLLSLLDKYKYQTIDLEGFACHRGSTFGGLGMKKQPSNEQFENIIAEKLNSFKFSNNIFVEAESANIGKCKIPHEFFNQM
KNSRRIEIIRSESNRLDELIDTYSVFKKEELQESVLRIKKRLGPQRTKIALESIHNEKWDLVCKSVLDYYDKCYEYEKVG
KTNIEIIDLTDKKYDKSILELINNVL
>Mature_346_residues
MYFKRKELEKFRCFKGPLIDVRSPSEYYKGHMPNSINIPLFDDDERSIIGTIYKKEGRRKAVIEGLKFFENKMELLLDNL
FKSIESHTTIPNKNNELFIRIYCSRGGMRSQSIGWLLDKFKLNIVTLNGGYKIYRRWVLDSFSNKLNLVVIGGKTGTGKT
RLLSLLDKYKYQTIDLEGFACHRGSTFGGLGMKKQPSNEQFENIIAEKLNSFKFSNNIFVEAESANIGKCKIPHEFFNQM
KNSRRIEIIRSESNRLDELIDTYSVFKKEELQESVLRIKKRLGPQRTKIALESIHNEKWDLVCKSVLDYYDKCYEYEKVG
KTNIEIIDLTDKKYDKSILELINNVL

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=293, Percent_Identity=30.3754266211604, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 40433; Mature: 40433

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFKRKELEKFRCFKGPLIDVRSPSEYYKGHMPNSINIPLFDDDERSIIGTIYKKEGRRK
CCCCHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHCCCHH
AVIEGLKFFENKMELLLDNLFKSIESHTTIPNKNNELFIRIYCSRGGMRSQSIGWLLDKF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHEEEE
KLNIVTLNGGYKIYRRWVLDSFSNKLNLVVIGGKTGTGKTRLLSLLDKYKYQTIDLEGFA
EEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCEEECCCCEE
CHRGSTFGGLGMKKQPSNEQFENIIAEKLNSFKFSNNIFVEAESANIGKCKIPHEFFNQM
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEECCCEEEEECCCCCCCEECCHHHHHHH
KNSRRIEIIRSESNRLDELIDTYSVFKKEELQESVLRIKKRLGPQRTKIALESIHNEKWD
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHH
LVCKSVLDYYDKCYEYEKVGKTNIEIIDLTDKKYDKSILELINNVL
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MYFKRKELEKFRCFKGPLIDVRSPSEYYKGHMPNSINIPLFDDDERSIIGTIYKKEGRRK
CCCCHHHHHHHHHCCCCEEECCCCHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHCCCHH
AVIEGLKFFENKMELLLDNLFKSIESHTTIPNKNNELFIRIYCSRGGMRSQSIGWLLDKF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHHHEEEE
KLNIVTLNGGYKIYRRWVLDSFSNKLNLVVIGGKTGTGKTRLLSLLDKYKYQTIDLEGFA
EEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCEEECCCCEE
CHRGSTFGGLGMKKQPSNEQFENIIAEKLNSFKFSNNIFVEAESANIGKCKIPHEFFNQM
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEECCCEEEEECCCCCCCEECCHHHHHHH
KNSRRIEIIRSESNRLDELIDTYSVFKKEELQESVLRIKKRLGPQRTKIALESIHNEKWD
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHH
LVCKSVLDYYDKCYEYEKVGKTNIEIIDLTDKKYDKSILELINNVL
HHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA