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The map label for this gene is hisA

Identifier: 126696195

GI number: 126696195

Start: 744118

End: 744885

Strand: Reverse

Name: hisA

Synonym: P9301_08571

Alternate gene names: 126696195

Gene position: 744885-744118 (Counterclockwise)

Preceding gene: 126696197

Following gene: 126696194

Centisome position: 45.37

GC content: 31.64

Gene sequence:

>768_bases
ATGAACCTAATACCAGCAATTGATTTAATGAATGGTAAGTGCGTAAGGCTTTTTAAAGGCGACTTTAATAAAAGAAAAGA
CTTTACCAAAGAGCCTCATGAGCAAGCTAAATTTTGGGAAAGCGAAGGGGCAAAATGTATACATATAGTTGATTTGGATG
CTGCAAAAACTGGATCCCCAACAAACGATAAATCAATAAAAAAGATTGCAAAAACAGTTAACATACCTATTCAAATAGGT
GGGGGGATAAGGTCTCAAGAAAGGATAGAACAATTATTTTCTTATGGTATTGAGAAAGTTATCATGGGAACCTCTGCAAT
AGAAAATAAAGAACTAGTTAAAGACTTATCAAATAAATATCCTGGAAGGATAATTGTTGGAATAGATGCAAAAAATGGAA
AAGTTAGTACAAGGGGTTGGCTTGAGCAATCTAATATTTTTGCCACAGATCTAGTAAAAGAGTTTTCTTCATTTAAAATT
GCTAGTTTTATTGTTACAGATATAAATACAGATGGGACGTTAGAAGGGACAAATGAAGAATTCATAAAAAGCATACTTGA
AATTACAGATATTCCAGTAATAGCCTCAGGAGGTGTTGGTTCAATTTCTGATTTATTATCCTTAGTAAAATTTGAAAACT
CTGGACTTTTTGGAGTAATTGTAGGTAAAGCTCTATATGAAAATAAATTCACGATAAAAGAAGCTAATAATGTATTGTCA
TCAGAGAGATTAAATGACTTTGATTTAAACAGAAATTACTACGCCTAA

Upstream 100 bases:

>100_bases
ATTTTCATATTCAAAAAATATTTAACTGAAGTAGTAATCTTAAATAGAATTACATATTGAATGGTACTACTAATAAGTAA
TTAATGAAAAGGACGATTCT

Downstream 100 bases:

>100_bases
AAGAGTATAAAAATTGATTTAAGGCTGGTGTTTGCAGTAATTGTTAGTTAATTTTGTATAAGAGATAAGAAATCTAGTCT
TGGAATTAATTTCAAAAAAA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNLIPAIDLMNGKCVRLFKGDFNKRKDFTKEPHEQAKFWESEGAKCIHIVDLDAAKTGSPTNDKSIKKIAKTVNIPIQIG
GGIRSQERIEQLFSYGIEKVIMGTSAIENKELVKDLSNKYPGRIIVGIDAKNGKVSTRGWLEQSNIFATDLVKEFSSFKI
ASFIVTDINTDGTLEGTNEEFIKSILEITDIPVIASGGVGSISDLLSLVKFENSGLFGVIVGKALYENKFTIKEANNVLS
SERLNDFDLNRNYYA

Sequences:

>Translated_255_residues
MNLIPAIDLMNGKCVRLFKGDFNKRKDFTKEPHEQAKFWESEGAKCIHIVDLDAAKTGSPTNDKSIKKIAKTVNIPIQIG
GGIRSQERIEQLFSYGIEKVIMGTSAIENKELVKDLSNKYPGRIIVGIDAKNGKVSTRGWLEQSNIFATDLVKEFSSFKI
ASFIVTDINTDGTLEGTNEEFIKSILEITDIPVIASGGVGSISDLLSLVKFENSGLFGVIVGKALYENKFTIKEANNVLS
SERLNDFDLNRNYYA
>Mature_255_residues
MNLIPAIDLMNGKCVRLFKGDFNKRKDFTKEPHEQAKFWESEGAKCIHIVDLDAAKTGSPTNDKSIKKIAKTVNIPIQIG
GGIRSQERIEQLFSYGIEKVIMGTSAIENKELVKDLSNKYPGRIIVGIDAKNGKVSTRGWLEQSNIFATDLVKEFSSFKI
ASFIVTDINTDGTLEGTNEEFIKSILEITDIPVIASGGVGSISDLLSLVKFENSGLFGVIVGKALYENKFTIKEANNVLS
SERLNDFDLNRNYYA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=33.75, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1788336, Length=252, Percent_Identity=24.6031746031746, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_PROM0 (A3PCK5)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091081.1
- ProteinModelPortal:   A3PCK5
- SMR:   A3PCK5
- STRING:   A3PCK5
- GeneID:   4911190
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_08571
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   PMAR167546:P9301ORF_0873-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 28220; Mature: 28220

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIPAIDLMNGKCVRLFKGDFNKRKDFTKEPHEQAKFWESEGAKCIHIVDLDAAKTGSP
CCCCCCEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCC
TNDKSIKKIAKTVNIPIQIGGGIRSQERIEQLFSYGIEKVIMGTSAIENKELVKDLSNKY
CCHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHCC
PGRIIVGIDAKNGKVSTRGWLEQSNIFATDLVKEFSSFKIASFIVTDINTDGTLEGTNEE
CCEEEEEEECCCCEECCCCCHHCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHH
FIKSILEITDIPVIASGGVGSISDLLSLVKFENSGLFGVIVGKALYENKFTIKEANNVLS
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHH
SERLNDFDLNRNYYA
HHCCCCCCCCCCCCC
>Mature Secondary Structure
MNLIPAIDLMNGKCVRLFKGDFNKRKDFTKEPHEQAKFWESEGAKCIHIVDLDAAKTGSP
CCCCCCEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCC
TNDKSIKKIAKTVNIPIQIGGGIRSQERIEQLFSYGIEKVIMGTSAIENKELVKDLSNKY
CCHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHCC
PGRIIVGIDAKNGKVSTRGWLEQSNIFATDLVKEFSSFKIASFIVTDINTDGTLEGTNEE
CCEEEEEEECCCCEECCCCCHHCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHH
FIKSILEITDIPVIASGGVGSISDLLSLVKFENSGLFGVIVGKALYENKFTIKEANNVLS
HHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHHHHHHHH
SERLNDFDLNRNYYA
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA