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The map label for this gene is ligA [H]

Identifier: 126696079

GI number: 126696079

Start: 658564

End: 659313

Strand: Reverse

Name: ligA [H]

Synonym: P9301_07411

Alternate gene names: 126696079

Gene position: 659313-658564 (Counterclockwise)

Preceding gene: 126696080

Following gene: 126696078

Centisome position: 40.16

GC content: 32.0

Gene sequence:

>750_bases
ATGAAGACTTATTTAGAAGAACGAATTGAATGGTATGACGATAATTATAGAAACGGTAATGCTTTAATCTCTGATAAGCA
GTTCGACCAACTTGAAAAAAATTTATTGAGAACAAACCCTAATTGTGATTACTTTAAAAAGAAAAATAAACTAGTTTTAC
CTTCATTGGAAAAGGATTCAATAGATGAATTTTTGAAAGGATTATTAGCAGATACCAGATTATTAATTGAACCAAAAATT
GATGGTTGTGCTGTTGCTTTGCAATATAGGGATGGAACATTGGAAAAAGCAATTTCAAGAAAAGGAACAGATGTTACTAG
TAAACTTATTAAAATCCAAGACATTCCCAATAATCTCCCTTTACAAGGAGTTCTTCAAGTTAGAGGTGAATTATACGCAC
CTAACCAAAGTCCAAATATCTCCCAGAGAATCGCTTCTGGATTTCTAAGAGCTAAAGAAGGATTTTCTCAAAGTCTTAGC
TTCTGCGCATTTCAAATACTTAATTCAACACTTAACCAATATGAGTCCAAAAAGAGTCTTTCTAAGCTTGGCTTCAAGAT
CCCTCAGGATATTTCATGCAACTTTACGAGCCAAGTTGAAGTATTTAGAAAACGATGGTTAGAAGGGAAGCTTTTTAGTG
AATATCCAACAGATGGAATAGTAGTAAAAATAAATTCTAGAAAATTACAACTGATTAGAGAAAAATCAAATTTAGATTAT
CCTTATTGGCAGGTGGCAATAAAACGTTAA

Upstream 100 bases:

>100_bases
TTCATTTCTTTTATGGACTGTTAAATATGAAGATAGAGAAAAATGGATTATGGATAAACCAAGTTGGATAACTATCCAAA
AACTTAAGCCATCGTATTAA

Downstream 100 bases:

>100_bases
TGGAATTAATACACCAGATTTTTCCTACTTGGCATATTTACTTGGATATGTTTTTGATTGGCTTTGCAACTTATGGTTTT
CTAAGAATTAAGAAAAAAAT

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKLVLPSLEKDSIDEFLKGLLADTRLLIEPKI
DGCAVALQYRDGTLEKAISRKGTDVTSKLIKIQDIPNNLPLQGVLQVRGELYAPNQSPNISQRIASGFLRAKEGFSQSLS
FCAFQILNSTLNQYESKKSLSKLGFKIPQDISCNFTSQVEVFRKRWLEGKLFSEYPTDGIVVKINSRKLQLIREKSNLDY
PYWQVAIKR

Sequences:

>Translated_249_residues
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKLVLPSLEKDSIDEFLKGLLADTRLLIEPKI
DGCAVALQYRDGTLEKAISRKGTDVTSKLIKIQDIPNNLPLQGVLQVRGELYAPNQSPNISQRIASGFLRAKEGFSQSLS
FCAFQILNSTLNQYESKKSLSKLGFKIPQDISCNFTSQVEVFRKRWLEGKLFSEYPTDGIVVKINSRKLQLIREKSNLDY
PYWQVAIKR
>Mature_249_residues
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKLVLPSLEKDSIDEFLKGLLADTRLLIEPKI
DGCAVALQYRDGTLEKAISRKGTDVTSKLIKIQDIPNNLPLQGVLQVRGELYAPNQSPNISQRIASGFLRAKEGFSQSLS
FCAFQILNSTLNQYESKKSLSKLGFKIPQDISCNFTSQVEVFRKRWLEGKLFSEYPTDGIVVKINSRKLQLIREKSNLDY
PYWQVAIKR

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=248, Percent_Identity=29.0322580645161, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 28727; Mature: 28727

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKLVLPSLEKDS
CCCHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCCCCC
IDEFLKGLLADTRLLIEPKIDGCAVALQYRDGTLEKAISRKGTDVTSKLIKIQDIPNNLP
HHHHHHHHHHCCEEEEECCCCCEEEEEEECCCHHHHHHHCCCCHHHHHHEEEECCCCCCC
LQGVLQVRGELYAPNQSPNISQRIASGFLRAKEGFSQSLSFCAFQILNSTLNQYESKKSL
HHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKLGFKIPQDISCNFTSQVEVFRKRWLEGKLFSEYPTDGIVVKINSRKLQLIREKSNLDY
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCCEEEEECCCCEEHHHHHCCCCC
PYWQVAIKR
CHHEEEECC
>Mature Secondary Structure
MKTYLEERIEWYDDNYRNGNALISDKQFDQLEKNLLRTNPNCDYFKKKNKLVLPSLEKDS
CCCHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCCCCC
IDEFLKGLLADTRLLIEPKIDGCAVALQYRDGTLEKAISRKGTDVTSKLIKIQDIPNNLP
HHHHHHHHHHCCEEEEECCCCCEEEEEEECCCHHHHHHHCCCCHHHHHHEEEECCCCCCC
LQGVLQVRGELYAPNQSPNISQRIASGFLRAKEGFSQSLSFCAFQILNSTLNQYESKKSL
HHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKLGFKIPQDISCNFTSQVEVFRKRWLEGKLFSEYPTDGIVVKINSRKLQLIREKSNLDY
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCCEEEEECCCCEEHHHHHCCCCC
PYWQVAIKR
CHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA