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The map label for this gene is phnD [H]

Identifier: 126696064

GI number: 126696064

Start: 645805

End: 646698

Strand: Reverse

Name: phnD [H]

Synonym: P9301_07261

Alternate gene names: 126696064

Gene position: 646698-645805 (Counterclockwise)

Preceding gene: 126696065

Following gene: 126696063

Centisome position: 39.39

GC content: 28.52

Gene sequence:

>894_bases
ATGTTTAATTTAAAGTATTTCCTTGTAAGTTCATCTCTATTATTTTCTGTTTTTTCATCACCTGTATTTTCAAATCCCAA
AGTTTTAAAAGTTGGAGCAATACCTGATCAAAACCAAGATGTTTTGGACAAAAGATTTAATTTATTTTCAAAAGAATTAT
CCAAACAACTTGATGTAGAAGTTAAATACATTCCTGTTATTAATTATATAGCAGCAGTAACTGGATTTAGAACTAAAGAT
TTAGATTTAGTTTGGTTTGGAGGTTTATCAGGAGTTCAAGCAAGATTACAAACACCTAATTCAATTGTCATAGCTCAAAG
AGATATCGATAAGGAATTTAAAAGTGTTTTTGTAGTAAACAAAAATTTAGAACTTAACTCAATTTCAAACATTAAAGGAC
TTAAAAAACTAAAGAATTTAAGATTTACTTTTGGCTCTGAAAACTCAACTTCTGGAAGATTAATGCCAGAATATTTTTTA
AATCAAGCAGGGGTAGAAATTAAACATTTTAAAGGGAAAAAAGCAGGTTTTAGTGGGAGTCATGATGCCACTATAGCTTT
AGTTAATAGTGGGGCATTTGATGCTGGAGCTTTAAATAAACAGGTTTGGGAAAACAATCTTAAAAATAATCCCAAAAGAA
CAAGTAATTTAGAATTATTCTGGATCACACCAGAATATGTTGACTATCATTGGGTAGCTCAAGGTGATCTTGAAAATAGA
TTCGGGGAGGGGTTTACAAAAGAACTTAAATCAGTAATTCTAAATTTAGATATAAAGCAAAAATCACATAAACAGATATT
AGATATGTTTAATGCAAAAAGATTTATAAAGGCAGAATCAAAACAATATAAAAATATAGAGGAAATCGGGAGGAAATTAA
ATAAAATTAGATGA

Upstream 100 bases:

>100_bases
ATGTATAAGAATATCTTGTGCAGCTTCAGAAATTAAAATAAAAGATGGACTTCAGAGGATTGAAAAAGCAATCTCTGAAT
ACTATTAATTTAACTAAGTT

Downstream 100 bases:

>100_bases
ATAATACTGTCTTAGAATTAGAAAATATATCTTATAAATACAAAAATGATCTGATCCTAAATAAAATAAATTTAAAAATA
AATTCTGGGGAAAAAATTGC

Product: phosphonate ABC transporter phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MFNLKYFLVSSSLLFSVFSSPVFSNPKVLKVGAIPDQNQDVLDKRFNLFSKELSKQLDVEVKYIPVINYIAAVTGFRTKD
LDLVWFGGLSGVQARLQTPNSIVIAQRDIDKEFKSVFVVNKNLELNSISNIKGLKKLKNLRFTFGSENSTSGRLMPEYFL
NQAGVEIKHFKGKKAGFSGSHDATIALVNSGAFDAGALNKQVWENNLKNNPKRTSNLELFWITPEYVDYHWVAQGDLENR
FGEGFTKELKSVILNLDIKQKSHKQILDMFNAKRFIKAESKQYKNIEEIGRKLNKIR

Sequences:

>Translated_297_residues
MFNLKYFLVSSSLLFSVFSSPVFSNPKVLKVGAIPDQNQDVLDKRFNLFSKELSKQLDVEVKYIPVINYIAAVTGFRTKD
LDLVWFGGLSGVQARLQTPNSIVIAQRDIDKEFKSVFVVNKNLELNSISNIKGLKKLKNLRFTFGSENSTSGRLMPEYFL
NQAGVEIKHFKGKKAGFSGSHDATIALVNSGAFDAGALNKQVWENNLKNNPKRTSNLELFWITPEYVDYHWVAQGDLENR
FGEGFTKELKSVILNLDIKQKSHKQILDMFNAKRFIKAESKQYKNIEEIGRKLNKIR
>Mature_297_residues
MFNLKYFLVSSSLLFSVFSSPVFSNPKVLKVGAIPDQNQDVLDKRFNLFSKELSKQLDVEVKYIPVINYIAAVTGFRTKD
LDLVWFGGLSGVQARLQTPNSIVIAQRDIDKEFKSVFVVNKNLELNSISNIKGLKKLKNLRFTFGSENSTSGRLMPEYFL
NQAGVEIKHFKGKKAGFSGSHDATIALVNSGAFDAGALNKQVWENNLKNNPKRTSNLELFWITPEYVDYHWVAQGDLENR
FGEGFTKELKSVILNLDIKQKSHKQILDMFNAKRFIKAESKQYKNIEEIGRKLNKIR

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=228, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 33809; Mature: 33809

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLKYFLVSSSLLFSVFSSPVFSNPKVLKVGAIPDQNQDVLDKRFNLFSKELSKQLDVE
CCCEEEEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCE
VKYIPVINYIAAVTGFRTKDLDLVWFGGLSGVQARLQTPNSIVIAQRDIDKEFKSVFVVN
EEEEHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCEEEEEECHHHHHHHEEEEEE
KNLELNSISNIKGLKKLKNLRFTFGSENSTSGRLMPEYFLNQAGVEIKHFKGKKAGFSGS
CCCCCCCCHHHHHHHHHHHCEEEECCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCC
HDATIALVNSGAFDAGALNKQVWENNLKNNPKRTSNLELFWITPEYVDYHWVAQGDLENR
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECHHHCCEEEEECCCHHHH
FGEGFTKELKSVILNLDIKQKSHKQILDMFNAKRFIKAESKQYKNIEEIGRKLNKIR
HCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MFNLKYFLVSSSLLFSVFSSPVFSNPKVLKVGAIPDQNQDVLDKRFNLFSKELSKQLDVE
CCCEEEEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCE
VKYIPVINYIAAVTGFRTKDLDLVWFGGLSGVQARLQTPNSIVIAQRDIDKEFKSVFVVN
EEEEHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCEEEEEECHHHHHHHEEEEEE
KNLELNSISNIKGLKKLKNLRFTFGSENSTSGRLMPEYFLNQAGVEIKHFKGKKAGFSGS
CCCCCCCCHHHHHHHHHHHCEEEECCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCC
HDATIALVNSGAFDAGALNKQVWENNLKNNPKRTSNLELFWITPEYVDYHWVAQGDLENR
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECHHHCCEEEEECCCHHHH
FGEGFTKELKSVILNLDIKQKSHKQILDMFNAKRFIKAESKQYKNIEEIGRKLNKIR
HCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]