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The map label for this gene is rpoD4 [H]

Identifier: 126695941

GI number: 126695941

Start: 525918

End: 526841

Strand: Reverse

Name: rpoD4 [H]

Synonym: P9301_06031

Alternate gene names: 126695941

Gene position: 526841-525918 (Counterclockwise)

Preceding gene: 126695942

Following gene: 126695940

Centisome position: 32.09

GC content: 38.96

Gene sequence:

>924_bases
GTGAGTTCATTAAGCGATTTTCTTGGTGAAATAGGTCGGCATCAACTTTTGACGCCGGAGAGAGAACTCACTATGGGCAG
AAAAGTCCAAGAAATGGTTGTGCTTGTTAATAGATGTCAAGAAGCAGGTGGCAAAGGGCCCGCTTGTGAATATTCCGAAG
CAGAAAGGAAAAAGATCAAAATTGGTGAAAAGGCAAAAAATGAGATGATAACCGCCAACTTAAGACTAGTAGTTAACCTT
GCTAAGAGATATCAAGGCAAGGGACTAGAACTGCTGGACTTAATTCAGGAAGGAACATTAGGTCTTACAAGGGCTGTAGA
AAAATATGATCCCTCTAGGGGGCATAGATTTTCCACCTATGCTTATTGGTGGATCAGGCAAGGATTAAATAGAGCATTAT
CAACTCAAAGTAGAACTATCAGGATACCTGTAAATATTAATGAAAAACTCACAAAATTAAGATCAGCAAAATCAAAACTT
ATGCAACTTAAGGGCATTCCACCCTCGACTGATGAGCTAGCTGAAGAGATGAAAATCACCAAGGAAGAAATTGATGAACT
TCTTTCCTGTGAATTGAGAAGCATTACTGTTAGTCTCCAAGGTACTGTCAAATCAAAGTCAGATCCCTCTGAGCTAGTTG
ATATTCTTCCAAGTGATCAAACTCCACCAATGGAATTAGCCGAATTAGCCGAAAGGACAGCCTCAGCCTGGAAGTTATTA
GATAAAGCAAATTTAACTGAAAAGGAAAGAAAAATAGTAAGTCTAAGATTTGGTTTAGATGGTTCTAACGAATGGAGAAC
TTTAGCTGAAGTTGCAAGGCATATGAGTTGTAGCAGGGAATATTGTCGACAAGTCGTTCAACGTGCTTTAAGAAAACTTA
GAAAAGCAGGAATACAGAATGGGCTGGTTGATAGTATTAGCTAA

Upstream 100 bases:

>100_bases
AATTAATTAATAATTATTAATTAATTATTAATTAATTATTACATGTATGGCTTATCACTGAATTAACTATAAGTTGAATA
TATCAACAAAGAGGTAAATC

Downstream 100 bases:

>100_bases
AAATTCAATCAAAAATCTAAATTCCATAGATAAGGATGACAGAGAATTACAAAAACAAAGAAAAAATTTATGATGCTGAA
GTTTTAGAAAGCTCAACATT

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSSLSDFLGEIGRHQLLTPERELTMGRKVQEMVVLVNRCQEAGGKGPACEYSEAERKKIKIGEKAKNEMITANLRLVVNL
AKRYQGKGLELLDLIQEGTLGLTRAVEKYDPSRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRSAKSKL
MQLKGIPPSTDELAEEMKITKEEIDELLSCELRSITVSLQGTVKSKSDPSELVDILPSDQTPPMELAELAERTASAWKLL
DKANLTEKERKIVSLRFGLDGSNEWRTLAEVARHMSCSREYCRQVVQRALRKLRKAGIQNGLVDSIS

Sequences:

>Translated_307_residues
MSSLSDFLGEIGRHQLLTPERELTMGRKVQEMVVLVNRCQEAGGKGPACEYSEAERKKIKIGEKAKNEMITANLRLVVNL
AKRYQGKGLELLDLIQEGTLGLTRAVEKYDPSRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRSAKSKL
MQLKGIPPSTDELAEEMKITKEEIDELLSCELRSITVSLQGTVKSKSDPSELVDILPSDQTPPMELAELAERTASAWKLL
DKANLTEKERKIVSLRFGLDGSNEWRTLAEVARHMSCSREYCRQVVQRALRKLRKAGIQNGLVDSIS
>Mature_306_residues
SSLSDFLGEIGRHQLLTPERELTMGRKVQEMVVLVNRCQEAGGKGPACEYSEAERKKIKIGEKAKNEMITANLRLVVNLA
KRYQGKGLELLDLIQEGTLGLTRAVEKYDPSRGHRFSTYAYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRSAKSKLM
QLKGIPPSTDELAEEMKITKEEIDELLSCELRSITVSLQGTVKSKSDPSELVDILPSDQTPPMELAELAERTASAWKLLD
KANLTEKERKIVSLRFGLDGSNEWRTLAEVARHMSCSREYCRQVVQRALRKLRKAGIQNGLVDSIS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=244, Percent_Identity=40.5737704918033, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1789098, Length=298, Percent_Identity=37.248322147651, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1789871, Length=240, Percent_Identity=29.1666666666667, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34668; Mature: 34536

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSDFLGEIGRHQLLTPERELTMGRKVQEMVVLVNRCQEAGGKGPACEYSEAERKKIK
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
IGEKAKNEMITANLRLVVNLAKRYQGKGLELLDLIQEGTLGLTRAVEKYDPSRGHRFSTY
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHH
AYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRSAKSKLMQLKGIPPSTDELAEEMKIT
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
KEEIDELLSCELRSITVSLQGTVKSKSDPSELVDILPSDQTPPMELAELAERTASAWKLL
HHHHHHHHHCHHHHEEEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DKANLTEKERKIVSLRFGLDGSNEWRTLAEVARHMSCSREYCRQVVQRALRKLRKAGIQN
HHCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLVDSIS
CCCCCCC
>Mature Secondary Structure 
SSLSDFLGEIGRHQLLTPERELTMGRKVQEMVVLVNRCQEAGGKGPACEYSEAERKKIK
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
IGEKAKNEMITANLRLVVNLAKRYQGKGLELLDLIQEGTLGLTRAVEKYDPSRGHRFSTY
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHH
AYWWIRQGLNRALSTQSRTIRIPVNINEKLTKLRSAKSKLMQLKGIPPSTDELAEEMKIT
HHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
KEEIDELLSCELRSITVSLQGTVKSKSDPSELVDILPSDQTPPMELAELAERTASAWKLL
HHHHHHHHHCHHHHEEEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DKANLTEKERKIVSLRFGLDGSNEWRTLAEVARHMSCSREYCRQVVQRALRKLRKAGIQN
HHCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GLVDSIS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA