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The map label for this gene is queD [H]

Identifier: 126695460

GI number: 126695460

Start: 119713

End: 120639

Strand: Reverse

Name: queD [H]

Synonym: P9301_01221

Alternate gene names: 126695460

Gene position: 120639-119713 (Counterclockwise)

Preceding gene: 126695462

Following gene: 126695459

Centisome position: 7.35

GC content: 34.09

Gene sequence:

>927_bases
ATGACTTCTACACAATCCAAACCATTACATGGAAAAGGACGTGAATGCGTCATAACTCGACGTGCCTGCTTTAGTTCTAG
TCACCGTTATTGGCTTCCTGAAAAAAGCCCAGAAGAAAATTTATCTCTTTTTGGAAAGTGCAGTATTGCACCAGGACATG
GTCATAATTATGAACTTATTGTTTCAATGGGTGGAGAACTAGACTCTGATGGAATGGTACTTAATCTCTCTGATGTAAAA
CACTCCATTAAAGATAAGGTTACTGGACAATTAGATTTTCGTTTTTTAAATGATGTCTGGCCTGAATTTAATGTTGATAA
TCAAGAGGGTATACTTCCAACAACTGAAGCATTAGTACAGGTGATTTGGCATCGTCTGAAGGATGATTTACCTCTTACAA
GTCTAAGACTTTATGAAAACCCAAATTTATGGGCAGATTATTTTGGAAAAAACATGGAAGCATTTTTAACAGTACAAACT
CATTTTGCAGCGGCTCATAGACTTGCAAAAGAAGAGATATCCTTTGATGAGAATAAAAAAATCTATGGGAAATGTGCCAG
AGTTAATGGACATGGTCATAACTATCTTGTTGATATTACTGTCAAAGGAGACATTGATAAAAGAACAGGAATGGTTTGCG
ACTTATCTGCCCTCCAAGATACAATTAACGATTTAATTGTTGAACAACTAGATCATACTTTTCTTAATAAAGACATCGAA
TTTTTCCATAATTGCGTTCCAACTGCTGAAAATATAGCTTTATATATTTCTGATATTCTTAAAAAACCAATACATCAACT
TGGTGCAACATTACACAAAATAAGACTCCAAGAGAGTCCAAATAATGCTGCAGAAATTTATGTTGATCAAAAGTTAACCA
ACTCATTGAAGTTGAAATTTGAAAATAGTTTAGTAACGCAAACTTGA

Upstream 100 bases:

>100_bases
CTTCTGCCCTTGATGGTATGAACTGTTTTTTCGATAATGGATTGTTCCATAAAGACAAAAGCGTATTTAAAACCTTAAGA
TAGTATTATCGCAGACAGGT

Downstream 100 bases:

>100_bases
GTATCCCAAGAGTAATAATTGTTATAGCATCAAGTCTTGATGGGAGAATTGCATTTCCTGGAGGTGGAGAATCACATCTT
GGAAGCGATGAAGATAAAAA

Product: 6-pyruvoyl tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Archaeosine biosynthesis protein queD [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELIVSMGGELDSDGMVLNLSDVK
HSIKDKVTGQLDFRFLNDVWPEFNVDNQEGILPTTEALVQVIWHRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQT
HFAAAHRLAKEEISFDENKKIYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQDTINDLIVEQLDHTFLNKDIE
FFHNCVPTAENIALYISDILKKPIHQLGATLHKIRLQESPNNAAEIYVDQKLTNSLKLKFENSLVTQT

Sequences:

>Translated_308_residues
MTSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELIVSMGGELDSDGMVLNLSDVK
HSIKDKVTGQLDFRFLNDVWPEFNVDNQEGILPTTEALVQVIWHRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQT
HFAAAHRLAKEEISFDENKKIYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQDTINDLIVEQLDHTFLNKDIE
FFHNCVPTAENIALYISDILKKPIHQLGATLHKIRLQESPNNAAEIYVDQKLTNSLKLKFENSLVTQT
>Mature_307_residues
TSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELIVSMGGELDSDGMVLNLSDVKH
SIKDKVTGQLDFRFLNDVWPEFNVDNQEGILPTTEALVQVIWHRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQTH
FAAAHRLAKEEISFDENKKIYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQDTINDLIVEQLDHTFLNKDIEF
FHNCVPTAENIALYISDILKKPIHQLGATLHKIRLQESPNNAAEIYVDQKLTNSLKLKFENSLVTQT

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Homo sapiens, GI4506331, Length=136, Percent_Identity=41.1764705882353, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115532226, Length=121, Percent_Identity=42.1487603305785, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115532224, Length=121, Percent_Identity=42.1487603305785, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19550074, Length=133, Percent_Identity=45.1127819548872, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24585378, Length=133, Percent_Identity=45.1127819548872, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 34950; Mature: 34819

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELI
CCCCCCCCCCCCCCEEEEEEHHHHCCCCCEECCCCCCCCCCEEEEEEEECCCCCCCEEEE
VSMGGELDSDGMVLNLSDVKHSIKDKVTGQLDFRFLNDVWPEFNVDNQEGILPTTEALVQ
EEECCCCCCCCEEEEHHHHHHHHHHHCCCEEHEEEHHHCCCCCCCCCCCCCCCHHHHHHH
VIWHRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQTHFAAAHRLAKEEISFDENKK
HHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCE
IYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQDTINDLIVEQLDHTFLNKDIE
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
FFHNCVPTAENIALYISDILKKPIHQLGATLHKIRLQESPNNAAEIYVDQKLTNSLKLKF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECHHCCCEEEEE
ENSLVTQT
CCCCCCCC
>Mature Secondary Structure 
TSTQSKPLHGKGRECVITRRACFSSSHRYWLPEKSPEENLSLFGKCSIAPGHGHNYELI
CCCCCCCCCCCCCEEEEEEHHHHCCCCCEECCCCCCCCCCEEEEEEEECCCCCCCEEEE
VSMGGELDSDGMVLNLSDVKHSIKDKVTGQLDFRFLNDVWPEFNVDNQEGILPTTEALVQ
EEECCCCCCCCEEEEHHHHHHHHHHHCCCEEHEEEHHHCCCCCCCCCCCCCCCHHHHHHH
VIWHRLKDDLPLTSLRLYENPNLWADYFGKNMEAFLTVQTHFAAAHRLAKEEISFDENKK
HHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCE
IYGKCARVNGHGHNYLVDITVKGDIDKRTGMVCDLSALQDTINDLIVEQLDHTFLNKDIE
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
FFHNCVPTAENIALYISDILKKPIHQLGATLHKIRLQESPNNAAEIYVDQKLTNSLKLKF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEEEECHHCCCEEEEE
ENSLVTQT
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]