BacMap

Definition	Clostridium difficile 630 chromosome, complete genome.
Accession	NC_009089
Length	4,290,252

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The map label for this gene is crt2 [H]

Identifier: 126698641

GI number: 126698641

Start: 1249945

End: 1250742

Strand: Direct

Name: crt2 [H]

Synonym: CD1057

Alternate gene names: 126698641

Gene position: 1249945-1250742 (Clockwise)

Preceding gene: 126698640

Following gene: 126698642

Centisome position: 29.13

GC content: 32.83

Gene sequence:

>798_bases
ATGAGTACAAGTGATGTTAAAGTTTATGAGAATGTAGCTGTTGAAGTAGATGGAAATATATGTACAGTGAAAATGAATAG
ACCTAAAGCCCTTAATGCAATAAATTCAAAGACTTTAGAAGAACTTTATGAAGTATTTGTAGATATTAATAATGATGAAA
CTATTGATGTTGTAATATTGACAGGGGAAGGAAAGGCATTTGTAGCTGGAGCAGATATTGCATACATGAAAGATTTAGAT
GCTGTAGCTGCTAAAGATTTTAGTATCTTAGGAGCAAAAGCTTTTGGAGAAATAGAAAATAGTAAAAAAGTAGTGATAGC
TGCTGTAAACGGATTTGCTTTAGGTGGAGGATGTGAACTTGCAATGGCATGTGATATAAGAATTGCATCTGCTAAAGCTA
AATTTGGTCAGCCAGAAGTAACTCTTGGAATAACTCCAGGATATGGAGGAACTCAAAGACTTACAAGATTGGTTGGAATG
GCAAAAGCAAAAGAATTAATCTTTACAGGTCAAGTTATAAAAGCTGATGAAGCTGAAAAAATAGGGCTAATAAATAGAGT
CGTTGAGCCAGACATTTTAATAGAAGAAGTTGAGAAATTAGCTAAGATAATAGCTAAAAATGCTCAGCTTGCAGTTAGAT
ACTCTAAAGAAGCAATACAACTTGGTGCTCAAACTGATATAAATACTGGAATAGATATAGAATCTAATTTATTTGGTCTT
TGTTTTTCAACTAAAGACCAAAAAGAAGGAATGTCAGCTTTCGTTGAAAAGAGAGAAGCTAACTTTATAAAAGGGTAA

Upstream 100 bases:

>100_bases
TGGAGATGTTCATAAAGTGCTTCCAGAACTTATCAGTCAGTTAAGTGTTGCAAAAGAAAAAGGTGAAGTTTTAGCTAACT
AATTTCTTGGGGAGGGTATT

Downstream 100 bases:

>100_bases
AAATCTAATTAAAAATATATTTGCTGTTAACTAGATTAAATAGTATTTTAATCTAGTTAACAGAATATAAATACTAAAAT
AACAGGAGGTTATAACTATG

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYEVFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLD
AVAAKDFSILGAKAFGEIENSKKVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
AKAKELIFTGQVIKADEAEKIGLINRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEAIQLGAQTDINTGIDIESNLFGL
CFSTKDQKEGMSAFVEKREANFIKG

Sequences:

>Translated_265_residues
MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYEVFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLD
AVAAKDFSILGAKAFGEIENSKKVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
AKAKELIFTGQVIKADEAEKIGLINRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEAIQLGAQTDINTGIDIESNLFGL
CFSTKDQKEGMSAFVEKREANFIKG
>Mature_264_residues
STSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYEVFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDA
VAAKDFSILGAKAFGEIENSKKVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGMA
KAKELIFTGQVIKADEAEKIGLINRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEAIQLGAQTDINTGIDIESNLFGLC
FSTKDQKEGMSAFVEKREANFIKG

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=240, Percent_Identity=44.1666666666667, Blast_Score=203, Evalue=1e-52,
Organism=Homo sapiens, GI68989263, Length=201, Percent_Identity=36.81592039801, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI4502327, Length=269, Percent_Identity=34.5724907063197, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI20127408, Length=182, Percent_Identity=38.4615384615385, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=30.7392996108949, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.203007518797, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI261878539, Length=109, Percent_Identity=44.954128440367, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI45643119, Length=216, Percent_Identity=30.0925925925926, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260275230, Length=216, Percent_Identity=30.0925925925926, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI260274832, Length=216, Percent_Identity=30.0925925925926, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI116235487, Length=196, Percent_Identity=29.0816326530612, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI157694516, Length=233, Percent_Identity=24.4635193133047, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI213417737, Length=233, Percent_Identity=24.4635193133047, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=28.1914893617021, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=28.1914893617021, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI221136756, Length=235, Percent_Identity=24.6808510638298, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221136753, Length=235, Percent_Identity=24.6808510638298, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221307494, Length=231, Percent_Identity=24.6753246753247, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI157694520, Length=209, Percent_Identity=24.4019138755981, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI25453481, Length=261, Percent_Identity=22.9885057471264, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI51538573, Length=261, Percent_Identity=22.9885057471264, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4757968, Length=260, Percent_Identity=23.4615384615385, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI49355787, Length=260, Percent_Identity=23.4615384615385, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=37.3493975903614, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=36.8627450980392, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=32.9545454545455, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=31.9230769230769, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=37.037037037037, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790281, Length=183, Percent_Identity=34.4262295081967, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=30.4511278195489, Blast_Score=107, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=41.8032786885246, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=42.6829268292683, Blast_Score=191, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17540714, Length=224, Percent_Identity=40.625, Blast_Score=162, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17558304, Length=195, Percent_Identity=37.9487179487179, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=31.3253012048193, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=41.2087912087912, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=41.2087912087912, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=41.2087912087912, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17534483, Length=222, Percent_Identity=31.5315315315315, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=226, Percent_Identity=31.4159292035398, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144157, Length=201, Percent_Identity=32.8358208955224, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=200, Percent_Identity=33, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=242, Percent_Identity=29.7520661157025, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=211, Percent_Identity=27.9620853080569, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=176, Percent_Identity=25.5681818181818, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=260, Percent_Identity=23.0769230769231, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=198, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129971, Length=270, Percent_Identity=45.1851851851852, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24653477, Length=270, Percent_Identity=45.1851851851852, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=40.7258064516129, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI21357171, Length=212, Percent_Identity=32.0754716981132, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650670, Length=228, Percent_Identity=32.4561403508772, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19921000, Length=196, Percent_Identity=35.2040816326531, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583077, Length=196, Percent_Identity=35.2040816326531, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583079, Length=196, Percent_Identity=35.2040816326531, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24654903, Length=184, Percent_Identity=29.8913043478261, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19920382, Length=224, Percent_Identity=29.9107142857143, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=24.4897959183673, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI28571729, Length=137, Percent_Identity=34.3065693430657, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28571730, Length=137, Percent_Identity=34.3065693430657, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24583165, Length=166, Percent_Identity=26.5060240963855, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28432; Mature: 28301

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYEVFVDINNDETIDVVIL
CCCCCEEEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEEE
TGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSKKVVIAAVNGFALGGGCEL
ECCCCEEEEECCCHHHHHHHHHHHCCCEEECHHHHCCCCCCCEEEEEEECCEEECCCCEE
AMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGMAKAKELIFTGQVIKADEAEK
EEEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEEECCEEECCCCHH
IGLINRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEAIQLGAQTDINTGIDIESNLFGL
HHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCCCCCCCCEEEE
CFSTKDQKEGMSAFVEKREANFIKG
EECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYEVFVDINNDETIDVVIL
CCCCEEEEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEEE
TGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSKKVVIAAVNGFALGGGCEL
ECCCCEEEEECCCHHHHHHHHHHHCCCEEECHHHHCCCCCCCEEEEEEECCEEECCCCEE
AMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGMAKAKELIFTGQVIKADEAEK
EEEEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEEECCEEECCCCHH
IGLINRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEAIQLGAQTDINTGIDIESNLFGL
HHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCCCCCCCCEEEE
CFSTKDQKEGMSAFVEKREANFIKG
EECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8001771 [H]