Click here to switch to the map view.

The map label for this gene is sbcD [H]

Identifier: 126698626

GI number: 126698626

Start: 1231739

End: 1232950

Strand: Direct

Name: sbcD [H]

Synonym: CD1042

Alternate gene names: 126698626

Gene position: 1231739-1232950 (Clockwise)

Preceding gene: 126698625

Following gene: 126698627

Centisome position: 28.71

GC content: 31.02

Gene sequence:

>1212_bases
ATGAGATTTATTCATACATCAGATTGGCATCTAGGGAAGTCCCTAGAAGGCCATTCTAGAATTGAGGAACAAGCAAAGTT
TTGTGAGGAATTTATAAAAATAGTAGAATCAAATGAAATAGATATGGTTATAATTGCTGGAGATGTATATGATACTTCTA
ATCCCCCAGCACAAGCAGAAAAACTATTTTATCAGACTGTATCAAGACTTGCCAATAATGGTCAGAGGTGTGTATTAATA
ATTTCAGGAAATCATGATAATCCAGAGAGACTCTCTGCAATAACACCTCTAGCACATGAACAGGGGATACTTATATATGG
TTATCCATTAAGTGCTACGATAGAAGCTAAGTATAAAGGGTTTGAAATAACATATGCAACTCAGGGATGTACAAAACTGA
ATATAAATGGAGAAAATATTGTGGTTGCTACATTACCTTATCCAAGTGAAAAAAGATTAAATGAAGTTTTTTCAAGTGAA
GATGAGTTTGAGAAACAAAAAAATTATTCTGAAAAGGTAGGAGATATATTTAGAAGTTTAGAAGAAAATTTTAGAAGTGA
TACTATAAATATAGCCGTATCACATATATTTGTAATAGGTGGAGAGAGTACTGAATCAGAAAGACCTATACAATTAGGTG
GAAGTTTTTTAGTTGAAAGAAAAGATTTGCCAGAAAAGGCTCAATATACTGCACTAGGTCATTTACATAAACAACAAAAA
GCTTCCGAACGTTTAAATGCATACTATTCTGGTTCTCCACTTCAATATAGCAAGGATGAAAGAGCGTATACCAAAGGGGC
ATACATAGTGGATATAAAAGCTGGTGAAAAACCAATAATTGAAGATGTATATTTTAATAATTACAAACCTATAGAAGTAT
TTAAATGTAATGGAATAGAAGAAGCACTAGATATATGTGAGCAAAATCAAGATAGAGAGATTTGGTCATATTTTGAAATA
AATACTGATGAGATAATATCACAGAATGAAATTAAAAAAATGAAGGAACTATTAAAAGACATAATAGAAATAAAACCAAT
AATAACATCTTGTTATGAACAAGAAAGTGTAGATATAAAAGAAAAGTCTATGGCAGAGTTGTTTAGAGAGTTCTATTCTT
TTAGTAAAGGTGTAGAGCCAAAAGGAGAACTTATGGATTTATTTCTAGATATAATAAGTGAAGAAGGTGAATCAGCAGAT
GAGACCAATTAG

Upstream 100 bases:

>100_bases
AGTAAAAGAGAAATGTATATATTTATTTGGAGTTGATGAAGCTGTTTGTTATTAGTTTTTATATATAAATTAAACCTAAA
AGATAGAGGAAGGGGAAAAA

Downstream 100 bases:

>100_bases
ATTAGAACTTACAGGGCTTAATAGCTATATTGATAAACAAGTTATAGACTTTGAAAAATTAATTGAAAGAGGTTTGTTTG
GGATATTTGGAACTACTGGA

Product: nuclease subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVIIAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLI
ISGNHDNPERLSAITPLAHEQGILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLVERKDLPEKAQYTALGHLHKQQK
ASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGEKPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEI
NTDEIISQNEIKKMKELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDIISEEGESAD
ETN

Sequences:

>Translated_403_residues
MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVIIAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLI
ISGNHDNPERLSAITPLAHEQGILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLVERKDLPEKAQYTALGHLHKQQK
ASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGEKPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEI
NTDEIISQNEIKKMKELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDIISEEGESAD
ETN
>Mature_403_residues
MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVIIAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLI
ISGNHDNPERLSAITPLAHEQGILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLVERKDLPEKAQYTALGHLHKQQK
ASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGEKPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEI
NTDEIISQNEIKKMKELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDIISEEGESAD
ETN

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=384, Percent_Identity=25, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46010; Mature: 46010

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVIIAGDVYDTSNPPAQAE
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
KLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQGILIYGYPLSATIEAKYKG
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECC
FEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSEDEFEKQKNYSEKVGDIFRSL
EEEEEEECCCEEEEECCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLVERKDLPEKAQYTALGHLHKQQK
HHHHCCCCEEEEEEEEEEECCCCCCCCCCCEECCCEEEECCCCCCHHHHHHHHHHHHHHH
ASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGEKPIIEDVYFNNYKPIEVFKCNGIE
HHHHHCEEECCCCCCCCCCCHHHHCCCEEEEEECCCCCCHHHHHCCCCCCEEEEECCCHH
EALDICEQNQDREIWSYFEINTDEIISQNEIKKMKELLKDIIEIKPIITSCYEQESVDIK
HHHHHHHCCCCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EKSMAELFREFYSFSKGVEPKGELMDLFLDIISEEGESADETN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVIIAGDVYDTSNPPAQAE
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
KLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQGILIYGYPLSATIEAKYKG
HHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECC
FEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSEDEFEKQKNYSEKVGDIFRSL
EEEEEEECCCEEEEECCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
EENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLVERKDLPEKAQYTALGHLHKQQK
HHHHCCCCEEEEEEEEEEECCCCCCCCCCCEECCCEEEECCCCCCHHHHHHHHHHHHHHH
ASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGEKPIIEDVYFNNYKPIEVFKCNGIE
HHHHHCEEECCCCCCCCCCCHHHHCCCEEEEEECCCCCCHHHHHCCCCCCEEEEECCCHH
EALDICEQNQDREIWSYFEINTDEIISQNEIKKMKELLKDIIEIKPIITSCYEQESVDIK
HHHHHHHCCCCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EKSMAELFREFYSFSKGVEPKGELMDLFLDIISEEGESADETN
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]