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The map label for this gene is gcvA [H]

Identifier: 126447576

GI number: 126447576

Start: 980034

End: 980906

Strand: Direct

Name: gcvA [H]

Synonym: BMA10247_A1043

Alternate gene names: 126447576

Gene position: 980034-980906 (Clockwise)

Preceding gene: 126446806

Following gene: 126446761

Centisome position: 41.66

GC content: 68.16

Gene sequence:

>873_bases
ATGCGCCGCCTCCCGCCCTTGAATGCCCTGCAGATCTTCGCGACGGTCGCGCGCCATCGCAGCTTCACGCGCGCGGCCGA
CGCGCTCTGCGTGACGCAGGGGGCGATCAGCCGCCAGATCCAGTCGCTCGAAGCGCATTACGGCTTTGCGCTCTTCATGC
GCCACGCGCGCGGCCTCACGTTGACGGTGGAGGGCGAGCAACTGCTGCCTGTCGTCGTCGAGAGCTTTGCGCGGATCGAG
GACATTTCACTGAAGCTCACGCGCCAGCGCACCGATCTCGCGCTGAAGGTGCCGACCTGCGTGATGCGCTGGGTACTGCC
GCGCATCATGCGTTTTCAGCGCGAGCATCCGGACCTGCACGTACAGATGACGACCACCTGGCGGCACGACGTCGATTTCC
AGAGCGAGCCGTTCGATGCGGCGATCGTCTACGGGATATCGCCCGGCCCGGACGTGGCCGCCGTGCCGCTGTTCGACGAA
CGGCTCACGCCGGTATGCGCGCCGGATCTGCTCGAGGGCAGGCCGCTGGCGCGCGTCGAGGATCTCGCGTGCCATACGCT
GCTGCACCCGACGCGTGATCATCGCGACTGGCGCCGGTGGCTCGACTACGCGGGCGCGGCCGGCGTCGACCCGGATCGCG
GGCCGAGCTTCGACTCGCTCGATCTGGCGACGAGCGCCGCGACGCAGGGCTTTGGCGTCGCGCTGGGCGATCTCACGCTC
AGCGAAGAGGATTTCGCCGCGCGGCGGCTCGCGATGCCGCTCGACATCGTGCAGCGGACGGGGGCGCGCTATTACTTCGT
CTATCCGGAGAGCGTGGCGCAGCAGCAGAAGATCCGGCGCTTCAGTGCGTGGCTCGACGCGAATCGCGATTGA

Upstream 100 bases:

>100_bases
CAACGTCCGCATTTCGCTTATCGTGACGGCTCGCCCGCAGGCATCGTCCGCCGCCATGCCTGTGCGTTCGCCCTGTTTTT
CGAGCCCGTCGTCGACCGCC

Downstream 100 bases:

>100_bases
CGGACGGCGCGCCGCGTTGCACGGGGCATGCCGCTTGCCGCCGGCCGCGACGTGCAGGCCGCCCGCGTCGCCCGCCGCGG
CGCGGTGGACCCTTCGCGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRRLPPLNALQIFATVARHRSFTRAADALCVTQGAISRQIQSLEAHYGFALFMRHARGLTLTVEGEQLLPVVVESFARIE
DISLKLTRQRTDLALKVPTCVMRWVLPRIMRFQREHPDLHVQMTTTWRHDVDFQSEPFDAAIVYGISPGPDVAAVPLFDE
RLTPVCAPDLLEGRPLARVEDLACHTLLHPTRDHRDWRRWLDYAGAAGVDPDRGPSFDSLDLATSAATQGFGVALGDLTL
SEEDFAARRLAMPLDIVQRTGARYYFVYPESVAQQQKIRRFSAWLDANRD

Sequences:

>Translated_290_residues
MRRLPPLNALQIFATVARHRSFTRAADALCVTQGAISRQIQSLEAHYGFALFMRHARGLTLTVEGEQLLPVVVESFARIE
DISLKLTRQRTDLALKVPTCVMRWVLPRIMRFQREHPDLHVQMTTTWRHDVDFQSEPFDAAIVYGISPGPDVAAVPLFDE
RLTPVCAPDLLEGRPLARVEDLACHTLLHPTRDHRDWRRWLDYAGAAGVDPDRGPSFDSLDLATSAATQGFGVALGDLTL
SEEDFAARRLAMPLDIVQRTGARYYFVYPESVAQQQKIRRFSAWLDANRD
>Mature_290_residues
MRRLPPLNALQIFATVARHRSFTRAADALCVTQGAISRQIQSLEAHYGFALFMRHARGLTLTVEGEQLLPVVVESFARIE
DISLKLTRQRTDLALKVPTCVMRWVLPRIMRFQREHPDLHVQMTTTWRHDVDFQSEPFDAAIVYGISPGPDVAAVPLFDE
RLTPVCAPDLLEGRPLARVEDLACHTLLHPTRDHRDWRRWLDYAGAAGVDPDRGPSFDSLDLATSAATQGFGVALGDLTL
SEEDFAARRLAMPLDIVQRTGARYYFVYPESVAQQQKIRRFSAWLDANRD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=37.8006872852234, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=32.404181184669, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=28.0276816608997, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=25.6849315068493, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787589, Length=270, Percent_Identity=27.4074074074074, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790262, Length=215, Percent_Identity=29.7674418604651, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI157672245, Length=187, Percent_Identity=31.0160427807487, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=186, Percent_Identity=27.9569892473118, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32789; Mature: 32789

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLPPLNALQIFATVARHRSFTRAADALCVTQGAISRQIQSLEAHYGFALFMRHARGLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LTVEGEQLLPVVVESFARIEDISLKLTRQRTDLALKVPTCVMRWVLPRIMRFQREHPDLH
EEECCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEE
VQMTTTWRHDVDFQSEPFDAAIVYGISPGPDVAAVPLFDERLTPVCAPDLLEGRPLARVE
EEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHCCCCCCCCHHHCCCCCHHHH
DLACHTLLHPTRDHRDWRRWLDYAGAAGVDPDRGPSFDSLDLATSAATQGFGVALGDLTL
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECEEE
SEEDFAARRLAMPLDIVQRTGARYYFVYPESVAQQQKIRRFSAWLDANRD
CCHHHHHHHHHCCHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRRLPPLNALQIFATVARHRSFTRAADALCVTQGAISRQIQSLEAHYGFALFMRHARGLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LTVEGEQLLPVVVESFARIEDISLKLTRQRTDLALKVPTCVMRWVLPRIMRFQREHPDLH
EEECCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEE
VQMTTTWRHDVDFQSEPFDAAIVYGISPGPDVAAVPLFDERLTPVCAPDLLEGRPLARVE
EEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHCCCCCCCCHHHCCCCCHHHH
DLACHTLLHPTRDHRDWRRWLDYAGAAGVDPDRGPSFDSLDLATSAATQGFGVALGDLTL
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECEEE
SEEDFAARRLAMPLDIVQRTGARYYFVYPESVAQQQKIRRFSAWLDANRD
CCHHHHHHHHHCCHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]