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The map label for this gene is rutD [H]

Identifier: 126447341

GI number: 126447341

Start: 921668

End: 922573

Strand: Direct

Name: rutD [H]

Synonym: BMA10247_A0975

Alternate gene names: 126447341

Gene position: 921668-922573 (Clockwise)

Preceding gene: 126447262

Following gene: 126447850

Centisome position: 39.18

GC content: 74.94

Gene sequence:

>906_bases
ATGAGCACGATTTCCACGACGACAACCGAGGCGGGCGCGGCGCCGTTCGCCGCGCTGCTCGCACGCTTTCCCGAGCGGCG
CGCGGCGGTGGGCGGCGCCGGCGTGATCGGCTATCGCGAGGCGGGCGAAGCCCATGCGCGGCGCGCCGGATGCGAACCCG
TCGTGCTGCTGCACGGGATCGGCTCGGGGGCGGCGTCGTGGGTGCGCCAGCTCGATGCGCTCGGCGCGACGCGTCGCGCG
CTCGCGTGGGACGCACCCGGCTATGGCGCATCGTCGCGCGTCGCAGCCGATTCGCCCGTCGCGGCGGACTACGCGGCGTC
GCTCGCCGCGTGGCTCGACGCGCTGCGCATCCAGCGCTGCGTGCTCGTCGGCCATTCGCTCGGCGCGATCATGGCCGGCG
CGTTTGCACGGATCGCGGGCGAGCGGCTGGCGGGATTGCTGCTGATTTCCCCCGCGGGCGGCTACGGCAGCGCGCCCGCC
GACGTGCGGGCCGGGCGGCGCGACAGCCGCCTTGCGTTGCTCGCGTCGCTCGGCCCGCAAGGGCTCGCCGCCGAGCGCAG
CGCGAACATGCTGTCCGCGCAGGCGAGCGGCGCGGCGCGCGAGTGGGTGCGCTGGAACATGGCGCGCATCGCGCCCGCGG
GCTACGCGCAGGCGACGCATCTGCTCGCGAACGCGGATCTCGCCTCCGATCTTGCCCATTGCCGCGGCCGCATCGCGGTC
GCGGTCGGCGGCGACGATGCGATCACGCCGCCCGCCGCATGCGAGCGGATCGCGCGGGCGGCGGGCGTCGGGCTGCAGGT
GATCGCGCGCGCGGGGCACGCCGGCTACGTCGAAGCGCCCGATGCATACAGCGCGTTGATCGACGCCTTTTGCCGTCAAT
GCGACGGATCGCGAGGCACACCATGA

Upstream 100 bases:

>100_bases
CTGATGTGCGTGATGCTCGGCTCGCCGAAGCCGATCACGCCGACGTATCCGCCGGATTCGCCGCTCGCGAAGATCAAGCG
CTGATGCACGGACGGCAACT

Downstream 100 bases:

>100_bases
GCGAGCCGCTGCTGAAGGACGCCGACGACGCGAGGAATGATTCGAACTACGTCGTACCGGGGCTCGAGCGCGGGCTGCGG
ATCCTGGCCGAGTTCTCGCC

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIQRCVLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPA
DVRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP

Sequences:

>Translated_301_residues
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIQRCVLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPA
DVRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP
>Mature_300_residues
STISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRAL
AWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIQRCVLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPAD
VRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAVA
VGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

Organism=Homo sapiens, GI50658087, Length=145, Percent_Identity=31.7241379310345, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558492, Length=132, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566936, Length=132, Percent_Identity=30.3030303030303, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24586387, Length=282, Percent_Identity=24.822695035461, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586391, Length=282, Percent_Identity=24.822695035461, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586389, Length=282, Percent_Identity=24.822695035461, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586385, Length=282, Percent_Identity=24.822695035461, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30556; Mature: 30425

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGI
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCEECCHHCCHHHHHHCCCCEEEEEEEC
GSGAASWVRQLDALGATRRALAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIQRC
CCHHHHHHHHHHHHCHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPADVRAGRRDSRLALLASLGPQ
HHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHCCCC
GLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCEEEE
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGT
EECCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
P
C
>Mature Secondary Structure 
STISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGI
CCCCCCCCCCCCHHHHHHHHHCCHHHHHCCCCCEECCHHCCHHHHHHCCCCEEEEEEEC
GSGAASWVRQLDALGATRRALAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIQRC
CCHHHHHHHHHHHHCHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPADVRAGRRDSRLALLASLGPQ
HHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHCCCC
GLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
CCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCEEEE
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGT
EECCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA