Definition Burkholderia mallei NCTC 10247 chromosome II, complete genome.
Accession NC_009079
Length 2,352,693

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The map label for this gene is hisM [H]

Identifier: 126447121

GI number: 126447121

Start: 1030709

End: 1031422

Strand: Direct

Name: hisM [H]

Synonym: BMA10247_A1090

Alternate gene names: 126447121

Gene position: 1030709-1031422 (Clockwise)

Preceding gene: 126447794

Following gene: 126446723

Centisome position: 43.81

GC content: 66.39

Gene sequence:

>714_bases
GTGATTCAGATCATCGGCCAATATTGGCAGAACTACCTGTTCAGCGACGGCTACCGGTTGAGCGGCCTCGCGATCACGCT
GTGGCTGCTCGTCGTGTCGCTCGGGCTCGGCTTCGGCCTCGCGGTGCCGCTCGCGATCGCGCGCGCCTCGCGCAACCGCG
CGGTGTCGGGCGCGGTGTGGCTCTACACGTACGTGTTCCGCGGCACGCCGCTCTACGTGCAACTGCTGCTCTGCTACACG
GGCATCTACAGCCTGAGCGTCGTGCACGATCATGCGTGGCTCGACAGCTTCTTTCGCAGCGCGATGAACTGCACGCTGCT
CGCGTTCACGCTGAACGAGTGCGCGTACGCGACCGAGATCTTCGCGGGCGCGATCAAGTCGACGGCGCCGGGCGAGATCG
AGGCGGGGCTCGCGTACGGCATGTCGCGCTTCAAGCTGTACACGCGGGTGATCCTGCCGTCGGCGCTGCGCCGCGCGCTG
CCGAGCTACAGCAACGAAGTGATCCTGATGCTGCACGCGACGACGCTCGCGTTCACGGCGACGGTGCCCGACATCCTGAA
GGTCACGCGCGACGTCAATTCGGCAACCTACGCATCGTTTCAGGCCTACGGGATCGCGGCCGCGCTGTACGCGGTCGTCG
TGTTCGCGCTGATCTGGCTGTTCCGCCGGATGGAGGCGCGCTGGCTCGCGTACCTGAGGCCGCAGGGGCATTGA

Upstream 100 bases:

>100_bases
TCGCGGCGGGCGCGATCTACCTCGCGATCACGACGTTGTCGAACCTCGTGTTGATGTACCTCGAAAAACGCTATTCGGCG
GGTGTGCGGAGGGTGGCGCT

Downstream 100 bases:

>100_bases
CCGGCGCGCGCGACGCGCGCGGGCGGCATCGATCAACGAAGCACGACGACGATCAAGGACGACGGCGAATGGACAAGCTG
ACAGTGGACGACCTGCACAA

Product: His/Glu/Gln/Arg/opine ABC transporter permease

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MIQIIGQYWQNYLFSDGYRLSGLAITLWLLVVSLGLGFGLAVPLAIARASRNRAVSGAVWLYTYVFRGTPLYVQLLLCYT
GIYSLSVVHDHAWLDSFFRSAMNCTLLAFTLNECAYATEIFAGAIKSTAPGEIEAGLAYGMSRFKLYTRVILPSALRRAL
PSYSNEVILMLHATTLAFTATVPDILKVTRDVNSATYASFQAYGIAAALYAVVVFALIWLFRRMEARWLAYLRPQGH

Sequences:

>Translated_237_residues
MIQIIGQYWQNYLFSDGYRLSGLAITLWLLVVSLGLGFGLAVPLAIARASRNRAVSGAVWLYTYVFRGTPLYVQLLLCYT
GIYSLSVVHDHAWLDSFFRSAMNCTLLAFTLNECAYATEIFAGAIKSTAPGEIEAGLAYGMSRFKLYTRVILPSALRRAL
PSYSNEVILMLHATTLAFTATVPDILKVTRDVNSATYASFQAYGIAAALYAVVVFALIWLFRRMEARWLAYLRPQGH
>Mature_237_residues
MIQIIGQYWQNYLFSDGYRLSGLAITLWLLVVSLGLGFGLAVPLAIARASRNRAVSGAVWLYTYVFRGTPLYVQLLLCYT
GIYSLSVVHDHAWLDSFFRSAMNCTLLAFTLNECAYATEIFAGAIKSTAPGEIEAGLAYGMSRFKLYTRVILPSALRRAL
PSYSNEVILMLHATTLAFTATVPDILKVTRDVNSATYASFQAYGIAAALYAVVVFALIWLFRRMEARWLAYLRPQGH

Specific function: Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788645, Length=236, Percent_Identity=60.1694915254237, Blast_Score=300, Evalue=7e-83,
Organism=Escherichia coli, GI1787086, Length=217, Percent_Identity=33.6405529953917, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1787030, Length=208, Percent_Identity=28.3653846153846, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788226, Length=193, Percent_Identity=32.1243523316062, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787087, Length=221, Percent_Identity=26.6968325791855, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788646, Length=191, Percent_Identity=30.8900523560209, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786873, Length=193, Percent_Identity=27.4611398963731, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786874, Length=217, Percent_Identity=27.1889400921659, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 26394; Mature: 26394

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIIGQYWQNYLFSDGYRLSGLAITLWLLVVSLGLGFGLAVPLAIARASRNRAVSGAVW
CCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHH
LYTYVFRGTPLYVQLLLCYTGIYSLSVVHDHAWLDSFFRSAMNCTLLAFTLNECAYATEI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHH
FAGAIKSTAPGEIEAGLAYGMSRFKLYTRVILPSALRRALPSYSNEVILMLHATTLAFTA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHH
TVPDILKVTRDVNSATYASFQAYGIAAALYAVVVFALIWLFRRMEARWLAYLRPQGH
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIQIIGQYWQNYLFSDGYRLSGLAITLWLLVVSLGLGFGLAVPLAIARASRNRAVSGAVW
CCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHH
LYTYVFRGTPLYVQLLLCYTGIYSLSVVHDHAWLDSFFRSAMNCTLLAFTLNECAYATEI
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHH
FAGAIKSTAPGEIEAGLAYGMSRFKLYTRVILPSALRRALPSYSNEVILMLHATTLAFTA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHH
TVPDILKVTRDVNSATYASFQAYGIAAALYAVVVFALIWLFRRMEARWLAYLRPQGH
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]