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The map label for this gene is citH [H]

Identifier: 126446162

GI number: 126446162

Start: 732088

End: 733425

Strand: Direct

Name: citH [H]

Synonym: BMA10247_A0795

Alternate gene names: 126446162

Gene position: 732088-733425 (Clockwise)

Preceding gene: 126446703

Following gene: 126445781

Centisome position: 31.12

GC content: 70.1

Gene sequence:

>1338_bases
ATGCACGAAGACAACCTGACGATGCCCGCCGACGGTGCGGGCGTCGCCCCGGCCGACGCCGTATCGGCGGCGGGCTTGCG
CACCGCGCGCGGCGGTGCGATCGCGGCCGCGGTGATCGGCAACTGGCTCGAATTCTTCGATTTCACCGTCTACGGTTTTT
TCGCGGTGATCATCGGCAAGCTGTATTTCCCGTCGCACGACGCGACGACGTCGCTGCTGCTGTCGGTCGCGACGTTCGCC
GCGGGCTTCTTCACGCGTCCGCTCGGCAGCATCGTGCTCGGCGTCTACGCGGACCGCCGCGGCCGCAAGGCGGCGCTGAA
CCTGACGATCCTGCTGATGGCGCTCGGCACCGGCATGATCGCGCTCGCGCCGACCTACGCGCAGATCGGCGTGCTCGCGC
CGGTGATCGTCGTGTGCGCGCGGCTGATGCAGGGCTTTTCGCAAGGCGGAGAGTTCGGCGCGGCGACGTCGACGCTCGTC
GAGCACGGCGGCGCCGCGCGCCGGGGCTTTTGCGCGAGCTGGCAGCTCGCGACGCAGGGCGGCGCGGCGCTGATGGGCTC
GGGGTTCGCGGCGCTGCTGTCGAACACGCTCGCGAAGGGCGCGCTGGAAAGCTGGGGCTGGCGCGTGCCGTTCGCGCTCG
GTGTGCTGATCGCGCCCGTCGGCATGTATCTGCGCCGCCGCCTGGCCGACGATGCGCCGGGCGCCGGCCACCACGGCATC
GACGGCGGCGTGCTGCGCGAGCTGTTCGCGCGGCACGGCCGCACGGTGCTGCTGCTGACGCTCACGGTGATGGGCGGCAC
CGTATCGACGTACATCCTGACGTTCTACATGCCGACCTACGCGATCCATACGCTCGGGCTGCCGATGAAGCTGTCGATGT
TCGTCGGCGTCGCGTCCGGCTGCGTGATGCTCGTCACCTGCCCGTTGTTCGGCTGGCTGTCCGACCGGATCGGCAGCCGG
CGCCTGCCGATCTTCGTCGGGCGCGGCGTGCTCGTCGTGCTGCTGTTTCCGGCGTTCGTGCTGATGAACCGCTATCCGTC
GCTCGCGGTCGTGATGCCGCTCACCGGGCTGATGCTGCTGTTCTATTCGATGGGCTCGGCGTCCGAGTTCGCGCTGATGT
GCGAATCGTTTCCGCGCCGCGTGCGCGCGACGGGCATCTCGATCGCCTATGCGCTCGCGGTGACGCTGTTCGGCGGCACC
GCCCAGCTCGTCGCGACGTGGCTCGTGCGCGTGACGGGCAGCACGCTCGCGCCGGCCGCTTACGTGGCCGTGTGCGTGAT
CGTGTCGCTCGTCGCGGTCGGGATGCTGCGCGAGACGGCGACGGAAACGGCCGACTGA

Upstream 100 bases:

>100_bases
CCGGTTCATGCCGCCCGACGCCAATCGAAGCCCCGCGAGCCGCGCAGGCGTCGGCCGCGGTTGCGCTTCCGGGTGACATA
TTCGACGAGGAGACAACGAC

Downstream 100 bases:

>100_bases
GCGCGTGGTGGCGCGGCGGCGCGGCGGCGCGGTGCGCGAGCGAATCGCGGCGCGGCGCCGCCGGTGTCGGCGGCGGGCCG
CGGCGGGGGAAATGGAAAGG

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGKLYFPSHDATTSLLLSVATFA
AGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMIALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLV
EHGGAARRGFCASWQLATQGGAALMGSGFAALLSNTLAKGALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSR
RLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLLFYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGT
AQLVATWLVRVTGSTLAPAAYVAVCVIVSLVAVGMLRETATETAD

Sequences:

>Translated_445_residues
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGKLYFPSHDATTSLLLSVATFA
AGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMIALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLV
EHGGAARRGFCASWQLATQGGAALMGSGFAALLSNTLAKGALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSR
RLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLLFYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGT
AQLVATWLVRVTGSTLAPAAYVAVCVIVSLVAVGMLRETATETAD
>Mature_445_residues
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGKLYFPSHDATTSLLLSVATFA
AGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMIALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLV
EHGGAARRGFCASWQLATQGGAALMGSGFAALLSNTLAKGALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASGCVMLVTCPLFGWLSDRIGSR
RLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLLFYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGT
AQLVATWLVRVTGSTLAPAAYVAVCVIVSLVAVGMLRETATETAD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=32.7102803738318, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1788942, Length=421, Percent_Identity=33.729216152019, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=28.6396181384248, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=32.8671328671329, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87082404, Length=374, Percent_Identity=24.331550802139, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082231, Length=216, Percent_Identity=25, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46680; Mature: 46680

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGK
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYFPSHDATTSLLLSVATFAAGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMI
HHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
ALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLVEHGGAARRGFCASWQLATQG
EECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCC
GAALMGSGFAALLSNTLAKGALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
CCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASG
CHHHHHHHHHHCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CVMLVTCPLFGWLSDRIGSRRLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLL
HHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGTAQLVATWLVRVTGSTLAPAA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHH
YVAVCVIVSLVAVGMLRETATETAD
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHEDNLTMPADGAGVAPADAVSAAGLRTARGGAIAAAVIGNWLEFFDFTVYGFFAVIIGK
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYFPSHDATTSLLLSVATFAAGFFTRPLGSIVLGVYADRRGRKAALNLTILLMALGTGMI
HHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
ALAPTYAQIGVLAPVIVVCARLMQGFSQGGEFGAATSTLVEHGGAARRGFCASWQLATQG
EECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCC
GAALMGSGFAALLSNTLAKGALESWGWRVPFALGVLIAPVGMYLRRRLADDAPGAGHHGI
CCHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DGGVLRELFARHGRTVLLLTLTVMGGTVSTYILTFYMPTYAIHTLGLPMKLSMFVGVASG
CHHHHHHHHHHCCCEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
CVMLVTCPLFGWLSDRIGSRRLPIFVGRGVLVVLLFPAFVLMNRYPSLAVVMPLTGLMLL
HHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FYSMGSASEFALMCESFPRRVRATGISIAYALAVTLFGGTAQLVATWLVRVTGSTLAPAA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHH
YVAVCVIVSLVAVGMLRETATETAD
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]