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The map label for this gene is citH [H]

Identifier: 126446160

GI number: 126446160

Start: 947304

End: 948545

Strand: Direct

Name: citH [H]

Synonym: BMA10247_A1004

Alternate gene names: 126446160

Gene position: 947304-948545 (Clockwise)

Preceding gene: 126447313

Following gene: 126446030

Centisome position: 40.26

GC content: 64.49

Gene sequence:

>1242_bases
GTGCTCGCGACTTCGGTCGGCAACGCGCTCGAATGGTTCGATATCGCCATTTATGCGTTCTTCGCCGTGCATATCGCGAA
GAATTTCTTCCCGACCGCGAACGAGACGGCGTCGATGCTGCTCACGTTCGGCTCGTTCGGCGCGTCGTATCTCGTGCGGC
CGATCGGCGGCATGGTGCTGGGCGCCTACGCGGACAAGCGCGGGCGCAAGGCGGCACTGATGATGTCGGTCGGTCTGATG
ATGATCGGCACGGCGATCATCGCGGTGATTCCGCCACATGCGTCGATCGGCCTGCTCGCGCCAGCAGGCGTGTTCATCGC
ACGGCTGATTCAGGGTTTTTCGGCGGGCGGCGAATTCGGCGCTTCGACGGCGATGCTGATCGAACATGCACCCGAGCGTC
GCGGCCTGCTCGCGAGCTGGCAGTTCACGACGCAAGGCCTCGCGACCCTGCTCGCGTCGACCTTCGGCTTCGCGCTCGCC
AAGCTGATGCCCGCCAGCGAGCTCTCCGCATGGGGCTGGCGCATGCCGTTCTTCTTCGGGCTGCTGATCGGGCCGGCGGG
TCTGTATCTGGGACGCTTTCTCGAAGATGCGGCCGATTACACCGAAGCCGAGCACACGGCCACGCCGGTGCGCGACGTAC
TCACGCGGCAGAAGGCGTTGCTGCTGACCTCGATCGGTGCGCTGACGGTGTCGACGGCGGTGAACTACCTGTTGCAGTAC
GTGCCGACGTTCGCGATCCGCGAATTGCATCTCGATGCATCGACGGGTTTCGCCGCGAGCATCGTCGCGGGGCTGATGCT
GACCTTGGTCACGCCGTTCGCGGGGCATCTGTCCGACAAAATCGGCCGCGTGAAGCAGATGTCGACTGCGGCGCTGCTAC
TGTTCGTGACGGGTTATCCGGCGTTCGCGTATGTCGTGTCGCATGTGTCGGTAGCGGCGTTGTTCGGGCTCGTCGCGTGG
CTTGCGCTGCTCAAGGCGGTGTATTTCGGCGCGCTGCCGGCGCTCATGTCGGAGATTTTCCCCACATCGACGCGCGTGAC
GAGCATTTCAATCAGCTACAACATCGGCGTGACGGTGTTCGGCGGTTTTACGCCGGCGATCGTCATCTGGCTGTCGAGCG
CGACGGGCAGCAAGGCGGCACCGAGCTTCTACATGATGTTCACGGCCGTGATCAGTCTCGCGGCGCTGGCGGCAGTGAGC
CGCGGGAAAGAGCCGCTGAGCTCGGCGGGCACGGCGGCCTGA

Upstream 100 bases:

>100_bases
CCGTAAAGCGCTTCTTCATGTTCGTCTTCTTCTCCGAAAACGAACTTTACTAGACTCCGGCTGGCCCTGTTTGTAGGGGG
CAGGTCACCTGACGCAAATC

Downstream 100 bases:

>100_bases
ATCCCGCACCGCTCACCGCTCCAGCGAGCGAATCAGTCTCACGTAGTGGGTCGGTTCGACAAGAAGGACCTCACGCGGCT
TGTGCGCCACTGCCTTCTGC

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MLATSVGNALEWFDIAIYAFFAVHIAKNFFPTANETASMLLTFGSFGASYLVRPIGGMVLGAYADKRGRKAALMMSVGLM
MIGTAIIAVIPPHASIGLLAPAGVFIARLIQGFSAGGEFGASTAMLIEHAPERRGLLASWQFTTQGLATLLASTFGFALA
KLMPASELSAWGWRMPFFFGLLIGPAGLYLGRFLEDAADYTEAEHTATPVRDVLTRQKALLLTSIGALTVSTAVNYLLQY
VPTFAIRELHLDASTGFAASIVAGLMLTLVTPFAGHLSDKIGRVKQMSTAALLLFVTGYPAFAYVVSHVSVAALFGLVAW
LALLKAVYFGALPALMSEIFPTSTRVTSISISYNIGVTVFGGFTPAIVIWLSSATGSKAAPSFYMMFTAVISLAALAAVS
RGKEPLSSAGTAA

Sequences:

>Translated_413_residues
MLATSVGNALEWFDIAIYAFFAVHIAKNFFPTANETASMLLTFGSFGASYLVRPIGGMVLGAYADKRGRKAALMMSVGLM
MIGTAIIAVIPPHASIGLLAPAGVFIARLIQGFSAGGEFGASTAMLIEHAPERRGLLASWQFTTQGLATLLASTFGFALA
KLMPASELSAWGWRMPFFFGLLIGPAGLYLGRFLEDAADYTEAEHTATPVRDVLTRQKALLLTSIGALTVSTAVNYLLQY
VPTFAIRELHLDASTGFAASIVAGLMLTLVTPFAGHLSDKIGRVKQMSTAALLLFVTGYPAFAYVVSHVSVAALFGLVAW
LALLKAVYFGALPALMSEIFPTSTRVTSISISYNIGVTVFGGFTPAIVIWLSSATGSKAAPSFYMMFTAVISLAALAAVS
RGKEPLSSAGTAA
>Mature_413_residues
MLATSVGNALEWFDIAIYAFFAVHIAKNFFPTANETASMLLTFGSFGASYLVRPIGGMVLGAYADKRGRKAALMMSVGLM
MIGTAIIAVIPPHASIGLLAPAGVFIARLIQGFSAGGEFGASTAMLIEHAPERRGLLASWQFTTQGLATLLASTFGFALA
KLMPASELSAWGWRMPFFFGLLIGPAGLYLGRFLEDAADYTEAEHTATPVRDVLTRQKALLLTSIGALTVSTAVNYLLQY
VPTFAIRELHLDASTGFAASIVAGLMLTLVTPFAGHLSDKIGRVKQMSTAALLLFVTGYPAFAYVVSHVSVAALFGLVAW
LALLKAVYFGALPALMSEIFPTSTRVTSISISYNIGVTVFGGFTPAIVIWLSSATGSKAAPSFYMMFTAVISLAALAAVS
RGKEPLSSAGTAA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=428, Percent_Identity=31.0747663551402, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=33.6585365853659, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788292, Length=375, Percent_Identity=25.3333333333333, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789941, Length=387, Percent_Identity=29.7157622739018, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082231, Length=411, Percent_Identity=23.8442822384428, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082404, Length=365, Percent_Identity=23.8356164383562, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 43457; Mature: 43457

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATSVGNALEWFDIAIYAFFAVHIAKNFFPTANETASMLLTFGSFGASYLVRPIGGMVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAYADKRGRKAALMMSVGLMMIGTAIIAVIPPHASIGLLAPAGVFIARLIQGFSAGGEFG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ASTAMLIEHAPERRGLLASWQFTTQGLATLLASTFGFALAKLMPASELSAWGWRMPFFFG
CHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHH
LLIGPAGLYLGRFLEDAADYTEAEHTATPVRDVLTRQKALLLTSIGALTVSTAVNYLLQY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPTFAIRELHLDASTGFAASIVAGLMLTLVTPFAGHLSDKIGRVKQMSTAALLLFVTGYP
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
AFAYVVSHVSVAALFGLVAWLALLKAVYFGALPALMSEIFPTSTRVTSISISYNIGVTVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEE
GGFTPAIVIWLSSATGSKAAPSFYMMFTAVISLAALAAVSRGKEPLSSAGTAA
CCCCCEEHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLATSVGNALEWFDIAIYAFFAVHIAKNFFPTANETASMLLTFGSFGASYLVRPIGGMVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAYADKRGRKAALMMSVGLMMIGTAIIAVIPPHASIGLLAPAGVFIARLIQGFSAGGEFG
HHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ASTAMLIEHAPERRGLLASWQFTTQGLATLLASTFGFALAKLMPASELSAWGWRMPFFFG
CHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHH
LLIGPAGLYLGRFLEDAADYTEAEHTATPVRDVLTRQKALLLTSIGALTVSTAVNYLLQY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPTFAIRELHLDASTGFAASIVAGLMLTLVTPFAGHLSDKIGRVKQMSTAALLLFVTGYP
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
AFAYVVSHVSVAALFGLVAWLALLKAVYFGALPALMSEIFPTSTRVTSISISYNIGVTVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEE
GGFTPAIVIWLSSATGSKAAPSFYMMFTAVISLAALAAVSRGKEPLSSAGTAA
CCCCCEEHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]