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The map label for this gene is phnU [H]

Identifier: 126446134

GI number: 126446134

Start: 851256

End: 852203

Strand: Direct

Name: phnU [H]

Synonym: BMA10247_A0898

Alternate gene names: 126446134

Gene position: 851256-852203 (Clockwise)

Preceding gene: 126446403

Following gene: 126446813

Centisome position: 36.18

GC content: 72.57

Gene sequence:

>948_bases
ATGTCGTCCTTATCTCACCTTGAGCCGCGGGCGGCGCTCGCCGCCGATCCCGCGGCCGCCGCCCGCATCCGCGCGTCGGC
CGCCGCGCACGACGCCGCCGCGCGTCGCCGCGAGCGGCGCGCGAGCGCACGCATCGTCGCGCTCGCGGCCGTGGTGCTCG
GCCCGCTCGTGCTGTATCCGCTCGTGCGGCTCGTGCTGCTGAGCGCGTTCGGCGCTCACGGCCCGACGCTCGCCGCATAC
CGCGCCTTCTTCGCGAGCCACGACGTGCGCGACGTGCTCGGCACGACGCTGTGGGTGCTGTTCGCGAGCGCGGGCACCGC
GTCGATCGTCGGCGTGCTGCTCGCCGCGCTGCTGTTCTTCCGGCCGTTTCCGGGCGCGTCGCTCGTCACGCGCTTCCTGG
AGCTGTACGTCGCGTTTCCGTCGTTTCTCGTCGCGTTCACGCTGATCTTCCTGTACGGCTCGCAAGGCTCGGTCAGCATC
GCGCTGCAGCGCGTCCTCGGCCTCGACGCGCCGCCGCTCGATTTCCTGTTCGGCCCGGGCGGCGTGATCCTCGCGCAGAC
CGTGTTCTACACGCCGTTCGTCGTGCGGCCGGCGCTCGCGTCGTTCGCCACGCTCGATGCGCGGCTCGTCGAAGCCGCCG
CCAGCCTCGGCGCGAACGGCTGGACGCTCGCGCGGCGCGTCGTGCTGCCGCTCGCGTGGCCGGGCATCGCGGCGGGCGTG
ATTCTCTGCTTCCTGCTGACGCTGAACGAATTCGGCATCCTGCTCGTGCTCGGCAGCGCGCGGCTCGTCACGCTGCCCGT
GGCGATCTACAGCAGCGCGACCGTCGACATCGACCTGCCGGCCGCGGCCGCGGGCGCGGTCGTGATGCTCGCGATGTCGC
TCACGCTCTATGCGCTGTACCGGCGGATGAGCCGCCGCGCGAACGAAGGAGGGCGCGATGTCCGCTGA

Upstream 100 bases:

>100_bases
GACGCGGCGTGCGACGAGCCGGTGCTCGTCACGATGCAGCGCGGCCGGGGCCCGGCACCCGAGCGCGGCGCGCGCGTATC
GCTCGATTGCGAGGCGGACG

Downstream 100 bases:

>100_bases
TTTCCCGGCTCGCGCCGCGCCGGTGCCGCGCCGCGAGCTGCGCTGGACCGATGCGGCCTCGCGCGTCGCCTCGCGTGCGC
TCGTCGCGCTCGCGGCCGCC

Product: 2-aminoethylphosphonate ABC transporter, permease protein

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSSLSHLEPRAALAADPAAAARIRASAAAHDAAARRRERRASARIVALAAVVLGPLVLYPLVRLVLLSAFGAHGPTLAAY
RAFFASHDVRDVLGTTLWVLFASAGTASIVGVLLAALLFFRPFPGASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSVSI
ALQRVLGLDAPPLDFLFGPGGVILAQTVFYTPFVVRPALASFATLDARLVEAAASLGANGWTLARRVVLPLAWPGIAAGV
ILCFLLTLNEFGILLVLGSARLVTLPVAIYSSATVDIDLPAAAAGAVVMLAMSLTLYALYRRMSRRANEGGRDVR

Sequences:

>Translated_315_residues
MSSLSHLEPRAALAADPAAAARIRASAAAHDAAARRRERRASARIVALAAVVLGPLVLYPLVRLVLLSAFGAHGPTLAAY
RAFFASHDVRDVLGTTLWVLFASAGTASIVGVLLAALLFFRPFPGASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSVSI
ALQRVLGLDAPPLDFLFGPGGVILAQTVFYTPFVVRPALASFATLDARLVEAAASLGANGWTLARRVVLPLAWPGIAAGV
ILCFLLTLNEFGILLVLGSARLVTLPVAIYSSATVDIDLPAAAAGAVVMLAMSLTLYALYRRMSRRANEGGRDVR
>Mature_314_residues
SSLSHLEPRAALAADPAAAARIRASAAAHDAAARRRERRASARIVALAAVVLGPLVLYPLVRLVLLSAFGAHGPTLAAYR
AFFASHDVRDVLGTTLWVLFASAGTASIVGVLLAALLFFRPFPGASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSVSIA
LQRVLGLDAPPLDFLFGPGGVILAQTVFYTPFVVRPALASFATLDARLVEAAASLGANGWTLARRVVLPLAWPGIAAGVI
LCFLLTLNEFGILLVLGSARLVTLPVAIYSSATVDIDLPAAAAGAVVMLAMSLTLYALYRRMSRRANEGGRDVR

Specific function: Probably part of the phnSTUV complex (TC 3.A.1.11.5) involved in 2-aminoethylphosphonate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=214, Percent_Identity=28.0373831775701, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786254, Length=233, Percent_Identity=28.3261802575107, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787369, Length=144, Percent_Identity=30.5555555555556, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017636
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33186; Mature: 33054

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSHLEPRAALAADPAAAARIRASAAAHDAAARRRERRASARIVALAAVVLGPLVLYP
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRLVLLSAFGAHGPTLAAYRAFFASHDVRDVLGTTLWVLFASAGTASIVGVLLAALLFF
HHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RPFPGASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSVSIALQRVLGLDAPPLDFLFGPG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHEECCC
GVILAQTVFYTPFVVRPALASFATLDARLVEAAASLGANGWTLARRVVLPLAWPGIAAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHH
ILCFLLTLNEFGILLVLGSARLVTLPVAIYSSATVDIDLPAAAAGAVVMLAMSLTLYALY
HHHHHHHHHCCCEEEEECCCHHEEEHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
RRMSRRANEGGRDVR
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSLSHLEPRAALAADPAAAARIRASAAAHDAAARRRERRASARIVALAAVVLGPLVLYP
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVRLVLLSAFGAHGPTLAAYRAFFASHDVRDVLGTTLWVLFASAGTASIVGVLLAALLFF
HHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RPFPGASLVTRFLELYVAFPSFLVAFTLIFLYGSQGSVSIALQRVLGLDAPPLDFLFGPG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHEECCC
GVILAQTVFYTPFVVRPALASFATLDARLVEAAASLGANGWTLARRVVLPLAWPGIAAGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHH
ILCFLLTLNEFGILLVLGSARLVTLPVAIYSSATVDIDLPAAAAGAVVMLAMSLTLYALY
HHHHHHHHHCCCEEEEECCCHHEEEHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
RRMSRRANEGGRDVR
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA