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The map label for this gene is pcaF-1 [H]

Identifier: 126445781

GI number: 126445781

Start: 736076

End: 737278

Strand: Direct

Name: pcaF-1 [H]

Synonym: BMA10247_A0798

Alternate gene names: 126445781

Gene position: 736076-737278 (Clockwise)

Preceding gene: 126446162

Following gene: 126445786

Centisome position: 31.29

GC content: 72.49

Gene sequence:

>1203_bases
ATGACCGAAGCATTCCTGTGTGACGCAATCCGCACGCCCATCGGCCGCTACGGCGGCGCGCTGGCGCCGGTGCGGGCCGA
CGACCTCGGCGCGGTGCCGCTCAAGGCGCTCGTCGAGCGCAACCGCGACGTCGACTGGGCCGCCGTCGACGACGTGATCT
ACGGCTGCGCGAATCAGGCCGGCGAGGACAACCGCAACGTCGCGCGCATGTCGCTGCTGCTCGCCGGCTTGCCGCACGCC
GTGCCCGGCGCGACGATCAACCGCCTCTGCGGCTCCGGGATGGACGCGATCGGCCTCGCCGCGCGCGCGATCAAGGCGGG
CGAGGCGAGCCTGATGATCGCCGGCGGCGTCGAGAGCATGAGCCGCGCGCCGTTCGTGACCGGCAAGGCGATGAGCGCGT
TCTCGCGCCAGGCCGAGATCTTCGATACGACCATCGGCTGGCGCTTCGTCAATCCGCTGATGAAACAGCGGTACGGCGTC
GATTCGATGCCGGAGACGGCCGAAAATGTCGCGACCGATTACCGCGTGAGCCGCACCGATCAGGACGCGTTCGCGCTGCG
CAGCCAGCAGAAGGCGGCGCGCGCGCAGGCCGACGGCACGCTCGCGCAGGAGATCGCGCCGGTGACGATCGCGCAGAAGC
AGGGCGAGCCGCTCGTCGTGGCGCGCGACGAGCATCCGCGCGAGACGACCCTCGAGGCGCTCGCGAAGCTCAAGGGCGTG
GTGCGCCCGGACGGCACGGTGACGGCGGGCAACGCGTCGGGCGTGAACGACGGTGCGTGCGCGCTGCTGGTGGCAAGCGA
GGCGGCGGCGCGCCGCCACGGGCTCGTGCCGCGCGCGCGTGTGCTGGGGATCGCGACGGCGGGCGTGGAGCCGCGGGTGA
TGGGGATCGGCCCGGCGCCGGCGACGCAGAAGCTGCTCGCGCGGCTGGGGATGACGCTCGACCAGTTCGACGTGATCGAG
CTGAACGAGGCGTTCGCGTCGCAGGGGCTCGCGGTGCTGCGGCTGCTGGGCGTCGCGGACGACGATGCGCGGGTGAATCC
GAACGGCGGCGCGATCGCGCTCGGGCATCCGCTTGGCGCGTCGGGCGCGCGGCTCGTGACGACCGCCACGTATCAGCTGC
ACCGCACGGGCGGCCGCTTCGCGCTCTGCACGATGTGCATCGGCGTCGGCCAGGGCATCGCGATCGCGATCGAGCGCGTG
TGA

Upstream 100 bases:

>100_bases
TCTTATGTTCATATAACGAACATTTGCCGTATGCATTCGAGGCGAGCGGAAGCGGCGGCATGTCGAGTCGGCTCGCGCCA
TCAGACCGACCGGAGACGAC

Downstream 100 bases:

>100_bases
TTGGCGCCGCCGCGCGACAGCGCGGCGGATGTCTACAGGCGGCCCGCGCTCGAAGCGTCGGGCCGTTTGTCTTGCCGGCC
CCGTGGCCGCTGTCCGGCCG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLAGLPHA
VPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKAMSAFSRQAEIFDTTIGWRFVNPLMKQRYGV
DSMPETAENVATDYRVSRTDQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALAKLKGV
VRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQFDVIE
LNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV

Sequences:

>Translated_400_residues
MTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLAGLPHA
VPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKAMSAFSRQAEIFDTTIGWRFVNPLMKQRYGV
DSMPETAENVATDYRVSRTDQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALAKLKGV
VRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQFDVIE
LNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
>Mature_399_residues
TEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLAGLPHAV
PGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKAMSAFSRQAEIFDTTIGWRFVNPLMKQRYGVD
SMPETAENVATDYRVSRTDQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALAKLKGVV
RPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQKLLARLGMTLDQFDVIEL
NEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=42.2784810126582, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=38.7909319899244, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI4557237, Length=409, Percent_Identity=34.2298288508557, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=38.9873417721519, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=30.8235294117647, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=28.9340101522843, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.3291770573566, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=45.6359102244389, Blast_Score=292, Evalue=2e-80,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.9057071960298, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=43.2098765432099, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=31.3868613138686, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=43.4673366834171, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=34.6534653465347, Blast_Score=221, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=33.9901477832512, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=32.093023255814, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=29.3827160493827, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=36.2282878411911, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=34.7394540942928, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.142144638404, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=39.1625615763547, Blast_Score=251, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=35.8024691358025, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=31.3679245283019, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24585051, Length=320, Percent_Identity=26.5625, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921506, Length=320, Percent_Identity=25.9375, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41752; Mature: 41621

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQA
CCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVARMSLLLAGLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
SRAPFVTGKAMSAFSRQAEIFDTTIGWRFVNPLMKQRYGVDSMPETAENVATDYRVSRTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEECCCC
QDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALAKLKGV
CHHHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAP
CCCCCCEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCHHEEEEEECCCCCEEEECCCCC
ATQKLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCEEEHHHHHHHCCHHHHHHHCCCCCCCEECCCCCEEEECCCCCC
SGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
CCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
TEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQA
CCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVARMSLLLAGLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
SRAPFVTGKAMSAFSRQAEIFDTTIGWRFVNPLMKQRYGVDSMPETAENVATDYRVSRTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEECCCC
QDAFALRSQQKAARAQADGTLAQEIAPVTIAQKQGEPLVVARDEHPRETTLEALAKLKGV
CHHHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAP
CCCCCCEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCHHEEEEEECCCCCEEEECCCCC
ATQKLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCEEEHHHHHHHCCHHHHHHHCCCCCCCEECCCCCEEEECCCCCC
SGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
CCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11741863 [H]