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The map label for this gene is dadA [C]

Identifier: 126445605

GI number: 126445605

Start: 835390

End: 836541

Strand: Direct

Name: dadA [C]

Synonym: BMA10247_A0882

Alternate gene names: 126445605

Gene position: 835390-836541 (Clockwise)

Preceding gene: 126446932

Following gene: 126446441

Centisome position: 35.51

GC content: 75.61

Gene sequence:

>1152_bases
GTGACCGATTCGCCGACCGACGTCATCGTGATCGGCGCGGGCATCGTCGGCGCCGCGTGCGCGCACGAGTTCGCGCTGCG
CGGGCTGCGCGTCGTCGTCGTCGACGACGGCGGCGGCGGCGCGACGGCCGCGGGCATGGGCCACCTGGTCGCGATGGACG
ACAACGCGGCCGAGCTCGCGCTCACGCATTACTCGATCGGGCTGTGGCGCGCGCTGCGCGACGCGATGCCGGAAGGCTGC
GCGTATCGCAACTGCGGCACGCTGTGGCTCGCGGCCGATGCGCACGAGATGGACCTCGCGCGCGCGAAGCAGGCGGCGCT
CGGCGCGCTCGGCGCGCTCGGCGCGCGCGGCGTCGCGGGCGAGCTGATCGGCCGCGCGGCGCTCGCCGCGCTCGAGCCGA
TGCTGCGCACGGACCTGGGCGGCGCGCTGAAGATTCCGGGCGACGGCATCCTGTACGCGCCCGCCGCCGCGCACTGGTTG
CTGCACCGGCTGCCGGGCGTCGCGCTGCGGCGCGCGAGGGCGGTCGCGCTCGAAGGCGCGCGCGTCGCGCTCGAAGGCGG
CGACGTGCTGCGCGCGAACGCCGTCGTCGTCGCGAACGGCGTCGCCGCGCGCGCGCTCGTGCCCGAGCTGCCGCTGCGGC
CGAAGAAGGGCCATCTGCTGATCACCGACCGCTATCCGGCGCGGGTGTCGCACCAGCTGGTCGAGCTCGGCTACGCGGCG
AGCGCGCACGCGAGCGACGGCACGTCGGTCGCGTTCAACGTGCAGCCGCGGCCGACGGGGCAATTGCTGATCGGCTCGTC
GCGGCAGTTCGACACCGAAGACCCGCGCGTCGAGGCGCCCGTGCTCGCGCGGATGCTGCGGCGCGCGCTCGGCTATCTGC
CCGCGCTCGCCGGCCTGAACGGCATCCGCGCGTGGACGGGCTTTCGCGCGGCGAGCCCCGACGGCCTGCCGCTGCTCGGC
GAGCATCCGTCGCGGCGCGGCGTGTGGCTCGCGCTCGGGCACGAGGGGCTTGGCGTGACGACCGCGCCGGGCAGCGCACG
CGTGCTCGCCGCGCTGATGTTCGGCGAGCGCGCGGCGATCGACGCCGAGCCGTATCGGCCGGCGCGTTTTCTGTCGACTT
CGAAATCGGCCGCGGCCGGAGAGCTTCGATGA

Upstream 100 bases:

>100_bases
AAGGCGGCGTCGTGCCGACGATCCGCGGCAGCGCGCACCTGAGCGCGGAGGCGACGCTCGTGATCGAGGACGACGATCCG
TTCGGCTGGGGCATCGCATC

Downstream 100 bases:

>100_bases
TCATTTATCTGGACCGCCGCGCGCTCGAGGTGGTCGACGGGACGACCGTCGCGGCCGCGCTCGCGCTCGCCGGCGACGAC
ACGGCGCGCACGTCGTGCAC

Product: oxidoreductase, FAD-binding family protein

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTDSPTDVIVIGAGIVGAACAHEFALRGLRVVVVDDGGGGATAAGMGHLVAMDDNAAELALTHYSIGLWRALRDAMPEGC
AYRNCGTLWLAADAHEMDLARAKQAALGALGALGARGVAGELIGRAALAALEPMLRTDLGGALKIPGDGILYAPAAAHWL
LHRLPGVALRRARAVALEGARVALEGGDVLRANAVVVANGVAARALVPELPLRPKKGHLLITDRYPARVSHQLVELGYAA
SAHASDGTSVAFNVQPRPTGQLLIGSSRQFDTEDPRVEAPVLARMLRRALGYLPALAGLNGIRAWTGFRAASPDGLPLLG
EHPSRRGVWLALGHEGLGVTTAPGSARVLAALMFGERAAIDAEPYRPARFLSTSKSAAAGELR

Sequences:

>Translated_383_residues
MTDSPTDVIVIGAGIVGAACAHEFALRGLRVVVVDDGGGGATAAGMGHLVAMDDNAAELALTHYSIGLWRALRDAMPEGC
AYRNCGTLWLAADAHEMDLARAKQAALGALGALGARGVAGELIGRAALAALEPMLRTDLGGALKIPGDGILYAPAAAHWL
LHRLPGVALRRARAVALEGARVALEGGDVLRANAVVVANGVAARALVPELPLRPKKGHLLITDRYPARVSHQLVELGYAA
SAHASDGTSVAFNVQPRPTGQLLIGSSRQFDTEDPRVEAPVLARMLRRALGYLPALAGLNGIRAWTGFRAASPDGLPLLG
EHPSRRGVWLALGHEGLGVTTAPGSARVLAALMFGERAAIDAEPYRPARFLSTSKSAAAGELR
>Mature_382_residues
TDSPTDVIVIGAGIVGAACAHEFALRGLRVVVVDDGGGGATAAGMGHLVAMDDNAAELALTHYSIGLWRALRDAMPEGCA
YRNCGTLWLAADAHEMDLARAKQAALGALGALGARGVAGELIGRAALAALEPMLRTDLGGALKIPGDGILYAPAAAHWLL
HRLPGVALRRARAVALEGARVALEGGDVLRANAVVVANGVAARALVPELPLRPKKGHLLITDRYPARVSHQLVELGYAAS
AHASDGTSVAFNVQPRPTGQLLIGSSRQFDTEDPRVEAPVLARMLRRALGYLPALAGLNGIRAWTGFRAASPDGLPLLGE
HPSRRGVWLALGHEGLGVTTAPGSARVLAALMFGERAAIDAEPYRPARFLSTSKSAAAGELR

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39678; Mature: 39547

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSPTDVIVIGAGIVGAACAHEFALRGLRVVVVDDGGGGATAAGMGHLVAMDDNAAELA
CCCCCCCEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEE
LTHYSIGLWRALRDAMPEGCAYRNCGTLWLAADAHEMDLARAKQAALGALGALGARGVAG
EEHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHH
ELIGRAALAALEPMLRTDLGGALKIPGDGILYAPAAAHWLLHRLPGVALRRARAVALEGA
HHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECHHHHHHHHHHCCCHHHHHHHHEEECCC
RVALEGGDVLRANAVVVANGVAARALVPELPLRPKKGHLLITDRYPARVSHQLVELGYAA
EEEEECCCEEEECEEEEECCCHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCC
SAHASDGTSVAFNVQPRPTGQLLIGSSRQFDTEDPRVEAPVLARMLRRALGYLPALAGLN
CCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GIRAWTGFRAASPDGLPLLGEHPSRRGVWLALGHEGLGVTTAPGSARVLAALMFGERAAI
HHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCC
DAEPYRPARFLSTSKSAAAGELR
CCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TDSPTDVIVIGAGIVGAACAHEFALRGLRVVVVDDGGGGATAAGMGHLVAMDDNAAELA
CCCCCCEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEEEECCCCCEEE
LTHYSIGLWRALRDAMPEGCAYRNCGTLWLAADAHEMDLARAKQAALGALGALGARGVAG
EEHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHH
ELIGRAALAALEPMLRTDLGGALKIPGDGILYAPAAAHWLLHRLPGVALRRARAVALEGA
HHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECHHHHHHHHHHCCCHHHHHHHHEEECCC
RVALEGGDVLRANAVVVANGVAARALVPELPLRPKKGHLLITDRYPARVSHQLVELGYAA
EEEEECCCEEEECEEEEECCCHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCC
SAHASDGTSVAFNVQPRPTGQLLIGSSRQFDTEDPRVEAPVLARMLRRALGYLPALAGLN
CCCCCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GIRAWTGFRAASPDGLPLLGEHPSRRGVWLALGHEGLGVTTAPGSARVLAALMFGERAAI
HHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCC
DAEPYRPARFLSTSKSAAAGELR
CCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]