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The map label for this gene is rusA [H]

Identifier: 126207987

GI number: 126207987

Start: 572506

End: 572874

Strand: Direct

Name: rusA [H]

Synonym: APL_0503

Alternate gene names: 126207987

Gene position: 572506-572874 (Clockwise)

Preceding gene: 126207986

Following gene: 126207988

Centisome position: 25.17

GC content: 37.67

Gene sequence:

>369_bases
ATGAGTGATTATTTAGAAATCGCATTGCCATATCCGCCGTCAGTTAATCATTATTGGCGGCATACTAAAGCCGGTACACA
TTACATCAGCAGAGAAGGGAAAGCGTTTCGGGCGAAAGTGCTGAATATTTGTAAGCAGTTTGACCCATTTAATGGCGCAG
TAAAAATTCAGATTTATGTTTATTATCCCGATAATCGCTCACGTGATCCTGATAACCTTCAAAAAGCCTTATTTGATGCG
CTGACGGCTTCGGGATTAATTAAAGATGATAACAACAAAATCATTAAAGATTATCGTGTAGTAGGAATGGATGAAATTCG
TAAAGGCGGAATGGTTGTAATTAAAATTAAGGGATTAGATGATGCGTAA

Upstream 100 bases:

>100_bases
TAGAGGCGTTGAGTGTTAATGACAGATAAAGATATTTTTATTGGCGGTTGTTTTTTAGTAAGTGTGTTTTGTGCGTTGAT
CAGTATTACGTGGGGTAAAA

Downstream 100 bases:

>100_bases
ATTTAGCGAGTTAAAACCACTAACAGAAGAGCAAGAAAGATTCGTTGATGAGAATATGTATGAGTGGGGAGCATGGATTC
GCAGCGGTCGTATTGATAAA

Product: endodeoxyribonuclease RusA

Products: NA

Alternate protein names: Holliday junction nuclease rusA; Holliday junction resolvase [H]

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MSDYLEIALPYPPSVNHYWRHTKAGTHYISREGKAFRAKVLNICKQFDPFNGAVKIQIYVYYPDNRSRDPDNLQKALFDA
LTASGLIKDDNNKIIKDYRVVGMDEIRKGGMVVIKIKGLDDA

Sequences:

>Translated_122_residues
MSDYLEIALPYPPSVNHYWRHTKAGTHYISREGKAFRAKVLNICKQFDPFNGAVKIQIYVYYPDNRSRDPDNLQKALFDA
LTASGLIKDDNNKIIKDYRVVGMDEIRKGGMVVIKIKGLDDA
>Mature_121_residues
SDYLEIALPYPPSVNHYWRHTKAGTHYISREGKAFRAKVLNICKQFDPFNGAVKIQIYVYYPDNRSRDPDNLQKALFDAL
TASGLIKDDNNKIIKDYRVVGMDEIRKGGMVVIKIKGLDDA

Specific function: Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of t

COG id: COG4570

COG function: function code L; Holliday junction resolvase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rusA family [H]

Homologues:

Organism=Escherichia coli, GI1786762, Length=117, Percent_Identity=46.1538461538462, Blast_Score=86, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016281
- InterPro:   IPR008822 [H]

Pfam domain/function: PF05866 RusA [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 13950; Mature: 13819

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYLEIALPYPPSVNHYWRHTKAGTHYISREGKAFRAKVLNICKQFDPFNGAVKIQIYV
CCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEE
YYPDNRSRDPDNLQKALFDALTASGLIKDDNNKIIKDYRVVGMDEIRKGGMVVIKIKGLD
EECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCEEEEEEEECHHHHCCCCEEEEEEECCC
DA
CC
>Mature Secondary Structure 
SDYLEIALPYPPSVNHYWRHTKAGTHYISREGKAFRAKVLNICKQFDPFNGAVKIQIYV
CCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEEE
YYPDNRSRDPDNLQKALFDALTASGLIKDDNNKIIKDYRVVGMDEIRKGGMVVIKIKGLD
EECCCCCCCHHHHHHHHHHHHHHCCCEECCCCCEEEEEEEECHHHHCCCCEEEEEEECCC
DA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA