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The map label for this gene is maeB [H]

Identifier: 126207970

GI number: 126207970

Start: 555322

End: 556590

Strand: Direct

Name: maeB [H]

Synonym: APL_0486

Alternate gene names: 126207970

Gene position: 555322-556590 (Clockwise)

Preceding gene: 126207968

Following gene: 126207971

Centisome position: 24.42

GC content: 45.86

Gene sequence:

>1269_bases
ATGAGCGATTTAAAACAACAAGCATTAGACTTCCACGAATTTCCGGTACCGGGCAAAATTTCGGTTACGCCGACTAAATC
ACTTGCAACGCAACATGATTTAGCCTTAGCGTATTCTCCGGGCGTTGCCGAACCTTGCTTGGCAATTGAGAAGGAACCCG
CTGCTGCTCGTCGTTATACCGCTCGTGGTAACTTAGTAGCGGTTATTTCAAACGGTACTGCGGTACTCGGTTTAGGTAAT
ATCGGTGCGTTGGCGTCCAAACCGGTAATGGAAGGTAAAGGCGTATTATTCAAAAAATTTGCCGGCGTAGATGTTTTTGA
TATCGAAATTAACGAACACGATCCGGATAAATTAGTCGATATTATCGCATCGTTAGAACCGACTTTCGGCGGAATCAACT
TAGAAGATATCAAAGCACCGGAATGTTTCTATATCGAGAAAAAATTACGTGAGCGTATGGGTATTCCTGTATTCCACGAC
GACCAACACGGCACGGCAATTATCGTAGGTGCGGCGGCAGTAAACGGTTTACAAATCGTAGGTAAAGATATTGGCGAAGT
GCGTTTAGTGGTAAACGGTGCGGGTGCGTCTGCGATTGCGTGTACCAACTTATTGCGTTCTTTAGGTTTCAAAAAAGAAA
ATATTACTATGTGCGATTCTAAAGGGGTAATTTACCAAGGTCGCGGTGATAATATGGATGAAACCAAACAATTCTATGCG
ATTGAAGATAACGGTTGGCGTACCCTTGCGGATGCGGTCGCGGGAGCGGACGTATTCTTAGGTTGTTCTAAAGCGGGCGC
ATTAACTCAAGATATGATCAAAACCATGGCGAAAGATCCGTTAATTCTTGCGTTAGCCAACCCTGTGCCGGAAACTACGC
CACACGAAGCGAAAGCCGTGCGTGCGGATGCGATCGTATGTACCGGTCGTTCGGATTATCCGAACCAAGTAAATAACGTA
CTTTGCTTCCCGTATTTATTCCGCGGTGCGTTAGACGTAGGTGCAACCACGATTAATGAAGAAATGAAAATGGCGGCGGC
ACACGCAATTGCAGATTTAGCAAAAGAGCCGGTTCCGTTTGAAGTATTATCAACTTACGGCGAATTATCGTTCGGTCCGG
ATTATGTAATCCCAACACCATTTGACCCTCGTTTAATTGTGGCGGTTTCATCAGCGGTTGCAAAAGCGGCAATGGATTCA
GGCGTTGCGACTCGCCCGATTACCGATTGGGATGCGTATGCAAAACAAGTGAAAGCGCTTATCGCTTAA

Upstream 100 bases:

>100_bases
TTTTATTTGTAGCATAAGAAGCGTATGAAATAATGTGCTACGGATAAAGATAAAGAAAATACACATCATCAAAAATCTTA
TTTATGTTAAGGATGTATCC

Downstream 100 bases:

>100_bases
CGGTTTAAAATAATTGAAAGAGGCGAGATTATTCGCCTCTTTTTGTTTTGCATTTTGGTAAAAAAGTGCGTAGAATATGC
ACTCGGCTTTTTCAGCCGAA

Product: NADP-dependent malic enzyme (NADP-ME)

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSDLKQQALDFHEFPVPGKISVTPTKSLATQHDLALAYSPGVAEPCLAIEKEPAAARRYTARGNLVAVISNGTAVLGLGN
IGALASKPVMEGKGVLFKKFAGVDVFDIEINEHDPDKLVDIIASLEPTFGGINLEDIKAPECFYIEKKLRERMGIPVFHD
DQHGTAIIVGAAAVNGLQIVGKDIGEVRLVVNGAGASAIACTNLLRSLGFKKENITMCDSKGVIYQGRGDNMDETKQFYA
IEDNGWRTLADAVAGADVFLGCSKAGALTQDMIKTMAKDPLILALANPVPETTPHEAKAVRADAIVCTGRSDYPNQVNNV
LCFPYLFRGALDVGATTINEEMKMAAAHAIADLAKEPVPFEVLSTYGELSFGPDYVIPTPFDPRLIVAVSSAVAKAAMDS
GVATRPITDWDAYAKQVKALIA

Sequences:

>Translated_422_residues
MSDLKQQALDFHEFPVPGKISVTPTKSLATQHDLALAYSPGVAEPCLAIEKEPAAARRYTARGNLVAVISNGTAVLGLGN
IGALASKPVMEGKGVLFKKFAGVDVFDIEINEHDPDKLVDIIASLEPTFGGINLEDIKAPECFYIEKKLRERMGIPVFHD
DQHGTAIIVGAAAVNGLQIVGKDIGEVRLVVNGAGASAIACTNLLRSLGFKKENITMCDSKGVIYQGRGDNMDETKQFYA
IEDNGWRTLADAVAGADVFLGCSKAGALTQDMIKTMAKDPLILALANPVPETTPHEAKAVRADAIVCTGRSDYPNQVNNV
LCFPYLFRGALDVGATTINEEMKMAAAHAIADLAKEPVPFEVLSTYGELSFGPDYVIPTPFDPRLIVAVSSAVAKAAMDS
GVATRPITDWDAYAKQVKALIA
>Mature_421_residues
SDLKQQALDFHEFPVPGKISVTPTKSLATQHDLALAYSPGVAEPCLAIEKEPAAARRYTARGNLVAVISNGTAVLGLGNI
GALASKPVMEGKGVLFKKFAGVDVFDIEINEHDPDKLVDIIASLEPTFGGINLEDIKAPECFYIEKKLRERMGIPVFHDD
QHGTAIIVGAAAVNGLQIVGKDIGEVRLVVNGAGASAIACTNLLRSLGFKKENITMCDSKGVIYQGRGDNMDETKQFYAI
EDNGWRTLADAVAGADVFLGCSKAGALTQDMIKTMAKDPLILALANPVPETTPHEAKAVRADAIVCTGRSDYPNQVNNVL
CFPYLFRGALDVGATTINEEMKMAAAHAIADLAKEPVPFEVLSTYGELSFGPDYVIPTPFDPRLIVAVSSAVAKAAMDSG
VATRPITDWDAYAKQVKALIA

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=398, Percent_Identity=25.3768844221106, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI62420880, Length=398, Percent_Identity=25.3768844221106, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI239049447, Length=398, Percent_Identity=25.3768844221106, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4505143, Length=343, Percent_Identity=25.3644314868805, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788806, Length=420, Percent_Identity=72.3809523809524, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI87081919, Length=293, Percent_Identity=24.9146757679181, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17537199, Length=360, Percent_Identity=25, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=271, Percent_Identity=29.520295202952, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24646388, Length=345, Percent_Identity=28.4057971014493, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281362672, Length=447, Percent_Identity=26.1744966442953, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24646386, Length=345, Percent_Identity=28.4057971014493, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281362674, Length=447, Percent_Identity=26.1744966442953, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356279, Length=447, Percent_Identity=26.1744966442953, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78707242, Length=350, Percent_Identity=27.4285714285714, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI78707238, Length=350, Percent_Identity=27.4285714285714, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI78707240, Length=350, Percent_Identity=27.4285714285714, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 44930; Mature: 44799

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLKQQALDFHEFPVPGKISVTPTKSLATQHDLALAYSPGVAEPCLAIEKEPAAARRYT
CCCHHHHHHHHHCCCCCCEEEECCCHHHHHHCCEEEEECCCCCCCHHHCCCCCHHHHEEE
ARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGVDVFDIEINEHDPDKLVD
CCCCEEEEEECCEEEEEECCCCCHHCCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHHH
IIASLEPTFGGINLEDIKAPECFYIEKKLRERMGIPVFHDDQHGTAIIVGAAAVNGLQIV
HHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCHHEE
GKDIGEVRLVVNGAGASAIACTNLLRSLGFKKENITMCDSKGVIYQGRGDNMDETKQFYA
CCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCCCCCEEEE
IEDNGWRTLADAVAGADVFLGCSKAGALTQDMIKTMAKDPLILALANPVPETTPHEAKAV
EECCCCHHHHHHHCCCHHEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHE
RADAIVCTGRSDYPNQVNNVLCFPYLFRGALDVGATTINEEMKMAAAHAIADLAKEPVPF
EEEEEEEECCCCCCHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCH
EVLSTYGELSFGPDYVIPTPFDPRLIVAVSSAVAKAAMDSGVATRPITDWDAYAKQVKAL
HHHHHCCCCCCCCCEEECCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IA
CC
>Mature Secondary Structure 
SDLKQQALDFHEFPVPGKISVTPTKSLATQHDLALAYSPGVAEPCLAIEKEPAAARRYT
CCHHHHHHHHHCCCCCCEEEECCCHHHHHHCCEEEEECCCCCCCHHHCCCCCHHHHEEE
ARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAGVDVFDIEINEHDPDKLVD
CCCCEEEEEECCEEEEEECCCCCHHCCCCCCCCCEEEEEECCCEEEEEEECCCCHHHHHH
IIASLEPTFGGINLEDIKAPECFYIEKKLRERMGIPVFHDDQHGTAIIVGAAAVNGLQIV
HHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCHHEE
GKDIGEVRLVVNGAGASAIACTNLLRSLGFKKENITMCDSKGVIYQGRGDNMDETKQFYA
CCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCCCCCCEEEE
IEDNGWRTLADAVAGADVFLGCSKAGALTQDMIKTMAKDPLILALANPVPETTPHEAKAV
EECCCCHHHHHHHCCCHHEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHE
RADAIVCTGRSDYPNQVNNVLCFPYLFRGALDVGATTINEEMKMAAAHAIADLAKEPVPF
EEEEEEEECCCCCCHHHCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCH
EVLSTYGELSFGPDYVIPTPFDPRLIVAVSSAVAKAAMDSGVATRPITDWDAYAKQVKAL
HHHHHCCCCCCCCCEEECCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
IA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]